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We narrowed to 50,496 results for: ang

Showing: 4051 - 4100 of 50496 results

  2. pEJS2782 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP

    Plasmid
    #240518
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with restriction enzyme sites in the 3' UTR for use as microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Use
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240518-plasmid-map-sequence-id-477187

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  3. Fc-E3

    Plasmid
    #181876
    Purpose
    Expresses murine Fc fusion to the first three EGF-like domains of human NOTCH3 WT
    Depositor
    Michael Wang
    Article
    Zhang et al Sci Rep. 2021 Aug 26;11(1):17246. doi: 1
    Insert
    Fc (murine) fused to NOTCH3 (human EGF-like domains 1-3) (NOTCH3 Human, Mouse)
    Use
    Tags
    Fc murine IgG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    181876-plasmid-map-sequence-id-357749

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  4. pBOB-3xFlag-hGpr75-p.Gln234*

    Plasmid
    #236748
    Purpose
    Express 3xFlag N-terminal tagged human GPR75 p.Gln234* mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
    Depositor
    Zhao Zhang
    Article
    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821
    Insert
    GPR75 (GPR75 Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Gln234 to STOP codon, CAA to TAA
    Promoter
    Available Since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    236748-plasmid-map-sequence-id-477901

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  5. pBOB-3xFlag-hGpr75-p.Ala110fs

    Plasmid
    #236747
    Purpose
    Express 3xFlag N-terminal tagged human GPR75 p.Ala110fs mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
    Depositor
    Zhao Zhang
    Article
    Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821
    Insert
    GPR75 (GPR75 Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Ala110fs, deletion of GCCAGTA
    Promoter
    Available Since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    236747-plasmid-map-sequence-id-477894

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  8. pCRISPR/eCas9-GPIDAF

    Plasmid
    #213710
    Purpose
    To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.
    Depositor
    Qilai Huang
    Article
    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:
    Insert
    EGFP
    Use
    CRISPR
    Tags
    3XFLAG and Twin-strep-tag
    Expression
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    213710-plasmid-map-sequence-id-422954

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  9. pCRISPR/eCas9-GPIBY55

    Plasmid
    #213709
    Purpose
    To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.
    Depositor
    Qilai Huang
    Article
    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:
    Insert
    EGFP
    Use
    CRISPR
    Tags
    3XFLAG and Twin-strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    213709-plasmid-map-sequence-id-422949

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  21. IBMc227

    Plasmid
    #161948
    Purpose
    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on TetR
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available Since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161948-plasmid-map-sequence-id-326357

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  22. IBMc367

    Plasmid
    #161947
    Purpose
    Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161947-plasmid-map-sequence-id-326355

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  23. IBMc228

    Plasmid
    #161946
    Purpose
    Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetR
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161946-plasmid-map-sequence-id-326343

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  27. pBUN411

    Plasmid
    #50581
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    maize codon optimized
    Promoter
    OsU3p and Ubi1p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50581-plasmid-map-sequence-id-311045

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  31. AXBEv2

    Plasmid
    #204606
    Purpose
    Mammalian expression of evolved adenine transversion base editor AXBEv2
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Promoter
    Available Since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204606-plasmid-map-sequence-id-404720

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  32. Td-CBEmax

    Plasmid
    #196600
    Purpose
    Mammalian expression of Td-CBEmax
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Insert
    bpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
    Use
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)
    Promoter
    Available Since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196600-plasmid-map-sequence-id-386793

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  34. ACBE

    Plasmid
    #204607
    Purpose
    Mammalian expression of adenine-to-cytosine base editor ACBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Promoter
    Available Since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204607-plasmid-map-sequence-id-404719

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  36. pHSN401

    Plasmid
    #50588
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    maize codon optimized
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50588-plasmid-map-sequence-id-75660

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  37. pCAT077

    Plasmid
    #180511
    Purpose
    Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180511-plasmid-map-sequence-id-359731

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  45. Lenti-sgRNA-Cre-GpNLuc

    Plasmid
    #208382
    Purpose
    Derived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.
    Depositor
    Gaorav Gupta
    Article
    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586
    Type
    Empty backbone
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available Since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208382-plasmid-map-sequence-id-412369

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  46. pAAV-TRE-DIO-GCaMP6s

    Plasmid
    #183809
    Purpose
    his plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene TetO promoter-DIO/flex-GCaMP6
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    183809-plasmid-map-sequence-id-366066

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Showing: 4051 - 4100 of 50496 results