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Showing: 1621 - 1640 of 114321 results

  1. pET-EIN-AlkBH5(66-292)

    Plasmid
    #125732
    Purpose
    Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.
    Depositor
    Vincenzo Venditti
    Article
    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055
    Insert
    AlkBH5(66-292) (ALKBH5 Human)
    Use
    Tags
    6xHis and EIN
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125732-plasmid-map-sequence-id-244619

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  2. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid
    #133794
    Purpose
    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    NmeCas9 and Nme2Cas9 sgRNA entry vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Amrani et al. Genome Biol…
    Promoter
    U6
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133794-plasmid-map-sequence-id-268076

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  13. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid
    #162277
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.
    Depositor
    Kazuhisa Nakayama
    Article
    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi
    Insert
    HiFi Cas9
    Use
    CRISPR
    Tags
    BPNLS and FLAG tag
    Expression
    Mammalian
    Mutation
    SpCas9 (R691A)
    Promoter
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    162277-plasmid-map-sequence-id-319794

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  14. pSYC-437

    Plasmid
    #163829
    Purpose
    Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.
    Depositor
    Seok-Yong Choi
    Article
    Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:
    Inserts
    Foxj1a Promoter
    GCaMP6s
    Use
    Multisite gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163829-plasmid-map-sequence-id-323196

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Showing: 1621 - 1640 of 114321 results