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pBJG1 T7-8xHis-OGT 5N5A
Plasmid#154280PurposeBacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationN322A, N356A, N390A, N424A, N458A mutations; bact…PromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-dSpCas9-HF1-VP64-6xHis
Plasmid#92118PurposeExpression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, Q926APromoterT7Available sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
His6tev CBP BCHZDeltaAL
Plasmid#99551PurposeBacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSGDepositorInsertmouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)
UseTagsHis6tevExpressionBacterialMutationreplaced 1557-1618 with SGGSGPromoterT7Available sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pdpy-30-miniCAFE
Plasmid#170115PurposeDpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.DepositorInsertMiniCAFE
UseCRISPRTagsExpressionMutationD8A, HNH-truncation (Δ495–609 aa) in CjCas9PromoterDpy-30Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6-SMT3-SopB
Plasmid#183676PurposeRecombinant Salmonella Typhimurium SopBDepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
UseTagsHis6-S. cerevisiae SMT3(2-101)ExpressionBacterialMutationPromoterT7Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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His-GINA3
Plasmid#74980PurposeExpresses His tagged GINA3 protein in bacteria.DepositorInsertRattus guanine nucleotide binding protein alpha inhibiting 3 (Gnai3) (Gnai3 )
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
His 3C Kinase dead MKK6 1-334
Plasmid#87882PurposeE. coli expression of MKK6 kinase deadDepositorInsertMKK6 (MAP2K6 Human)
UseTags3C protease site and 6xHisExpressionBacterialMutationK82A, kinase dead mutantPromoterT7Available sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-His-GvpC-Calpain
Plasmid#153295PurposeExpresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coliDepositorInsertCalpain-sensitive Gas vesicle protein C (his-tagged)
UseTagsHis-tagExpressionBacterialMutation1) Contains an extra glycine after the methionine…PromoterT7Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXB4 (human) HIS-tag pET new RBS
Plasmid#8525DepositorInsertHOXB4 (HOXB4 Human)
UseTagsHis and T7ExpressionBacterialMutationContains improved Ribosomal binding site; makes …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C F87E
Plasmid#238074PurposeBacterial expression of human TTR mutantsDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S S85C F87EPromoterT7Available sinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ce iPGM-C-HiBiT
Plasmid#207120PurposeBacterially expressed iPGM-HiBiT for Ni-NTA purificationDepositorInsertC. elegans-iPGM HiBIT His10 (ipgm-1 Nematode)
UseTagsHiBiT Tag and Thrombin cleavage site + 10x His Ta…ExpressionBacterialMutationCodon optmization for expression in BL21DE3PromoterT7 PromotorAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
E. coli Lig-N-HiBiT
Plasmid#215437PurposeBacterially expressed E. coli Lig-N-HiBiT for Ni-NTA purificationDepositorInsertNAD-dependent DNA ligase LigA [Escherichia coli], N-HiBiT, N-10XHis.Thrombin
UseTags10xHis-tag with thrombin site and HiBiT TagExpressionBacterialMutationCodon Optimized for bacterial expression, T7 gene…PromoterT7 PromotorAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
E. coli Lig-C-HiBiT
Plasmid#215438PurposeBacterially expressed E. coli Lig-C-HiBiT for Ni-NTA purificationDepositorInsertNAD-dependent DNA ligase LigA [Escherichia coli], C-HiBiT, C-10XHis.Thrombin
UseTags10xHis-tag with thrombin site and HiBiT TagExpressionBacterialMutationCodon Optimized for bacterial expression, T7 gene…PromoterT7 PromotorAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pINQ-ON10
Plasmid#198689PurposePeriplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)DepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody ON10
UseTags6xHis tag, HA tag, OmpA signal peptide, and Ribos…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINQ-NbH4
Plasmid#198690PurposePeriplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)DepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody H4
UseTags6xHis tag, Avitag, OmpA signal peptide, and Ribos…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
hzHDAC10
Plasmid#225542PurposeExpresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.DepositorInsertHistone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
UseTagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationMutated A24E and D94APromoterT7Available sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) C90A, T172C
Plasmid#217983Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationC90A, T172CPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) T160C
Plasmid#220233Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationT160CPromoterT7Available sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(85-160)
Plasmid#217984Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-100)
Plasmid#217978Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6GB1-CREB(1-280) C90A
Plasmid#217982Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationC90APromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) A50C,C90A
Plasmid#217985Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationA50C, C90APromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280)
Plasmid#217981Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(160-280)
Plasmid#217980Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tev-IntC-P53(304-393)
Plasmid#200315Purposebacterial expression of P53 TETCTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
UseTagsHis6GB1tev and designed intein CExpressionBacterialMutationPromoterT7Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN554
Plasmid#177361PurposeExpresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tagDepositorInsertKIF1A (KIF1A Human)
UseTagsLeucine zipper, StrepII tag, and mScarletExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppil2.280.457.Y389H
Plasmid#137658PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppil2 (PPIL2 Human)
UseTags(His)6-thrombinExpressionBacterialMutation280-457-Y389HPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppil2.280.457.Y389W
Plasmid#137657PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppil2 (PPIL2 Human)
UseTags(His)6-thrombinExpressionBacterialMutation280-457-Y389WPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
AF10_OM-LZ
Plasmid#111753PurposeBacterial expression for structure determinationDepositorInsertMLLT10 (MLLT10 Human)
UseTags6x His - TEVExpressionBacterialMutationContains the OM-LZ region of AF10 (residues 720-7…PromoterT7Available sinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
WT USP7 TRAF
Plasmid#227226PurposeExpresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationaa 62-205PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
FCM101
Plasmid#194298PurposeE. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6DepositorInsertFCM101
UseTagsHis tag, FLAG tagExpressionBacterialMutationPromoterT7Available sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-NCBD + ACTR
Plasmid#99335PurposeBacterial coexpression of CBP NCBD and NCoA3 binding domainsDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)
Plasmid#99343PurposeBacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TADDepositorInsertsUseTagsHis6GB1ExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
mature ISG15 C-term. domain (79-157)
Plasmid#110764PurposeBacterial expression of proISG15 C-term. domainDepositorInsertISG15 (ISG15 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)
Plasmid#99342PurposeBacterial expression of mouse CBP TAZ2 domain by coexpression with HisGB1 tagged STAT1 TADDepositorInsertsUseTagsHis6GB1ExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
proISG15 C-term. domain (79-165)
Plasmid#110763PurposeBacterial expression of proISG15 C-term. domainDepositorInsertISG15 (ISG15 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
His6-SMT3-SopB(C460S)
Plasmid#183677PurposeRecombinant Salmonella Typhimurium SopB (catalytically inactive)DepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
UseTagsHis6-S. cerevisiae SMT3(2-101)ExpressionBacterialMutationC460SPromoterT7Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PKA-N-HiBiT
Plasmid#211377PurposeBacterially expressed HiBiT-PKA for Ni-NTA purificationDepositorInsertHuman protein kinase cAMP-activated catalytic subunit alpha (PRKACA) transcript variant 1, with N-terminal HiBiT (PRKACA Human)
UseTags10xHis-tag with thrombin site and HiBiT TagExpressionBacterialMutationPromoterT7 PromotorAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
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RNF146
Plasmid#228448PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertRNF146 (RNF146 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) T2C, C90A, T198C
Plasmid#220235Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationT2C, C90A, T198CPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) C90A, T172C, T259C
Plasmid#220234Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationC90A, T172C, T259CPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN687
Plasmid#177363PurposeExpresses human KIF1A(1-393)(P305L) fused with leucine zipper and His tagDepositorInsertKIF1A (KIF1A Human)
UseTagsHis tag and Leucine zipperExpressionBacterialMutationchanged Proline 305 to LeucinePromoterT7Available sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Met1 diUb (1-152, R42W)
Plasmid#110767PurposeBacterial expression of Met1 diUbDepositorInsertUBB (UBB Human)
UseTagsN-His6-3CExpressionBacterialMutationR42WPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
cryS96
Plasmid#67519PurposeαB-crystallin minipeptide fused to ELP (S96).DepositorInsertmini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Human, Synthetic)
UseTagselastin-like polypeptide (ELP) S96ExpressionBacterialMutationmini-αB-crystallin contains residues 73–92 of aB-…PromoterT7Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
MEK1C222
Plasmid#164638PurposeBacterial expression plasmid for His6-MEK1C222DepositorInsertmitogen-activated protein kinase kinase 1 (MAP2K1 Human)
UseTagsHis6-tagExpressionBacterialMutationG(-19)F/S222C/C277S/C376SPromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-Hif1a(776-826)_FIH
Plasmid#99508PurposeBacterial expression of hydroxylated Hif1a TAD (776-826)DepositorUseTagsHis6GB1ExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
MEK1C218
Plasmid#164639PurposeBacterial expression plasmid for His6-MEK1C218DepositorInsertmitogen-activated protein kinase kinase 1 (MAP2K1 Human)
UseTagsHis6-tagExpressionBacterialMutationG(-19)F/S218C/C277S/C376SPromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only
