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Showing: 4101 - 4150 of 5577 results

  2. pCAS9id_TRP

    Plasmid
    #141254
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141254-plasmid-map-sequence-id-308894

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  3. pOpen-Exonuclease III E. coli

    Plasmid
    #165571
    Purpose
    Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Exonuclease III E. coli
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165571-plasmid-map-sequence-id-340929

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  5. PX400 Campylobacter lari CF89-12 Cas9

    Plasmid
    #68336
    Purpose
    Campylobacter lari CF89-12 Cas9
    Depositor
    Feng Zhang
    Insert
    ClCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68336-plasmid-map-sequence-id-119558

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  10. Tet-O-FUW-mCHERRY-PURO

    Plasmid
    #251739
    Purpose
    Overexpression construct expressing mCherry-PURO and ORF-BC
    Depositor
    Andrea Califano
    Insert
    mCHERRY
    Use
    Lentiviral
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    251739-plasmid-map-sequence-id-501488

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  11. Tet-O-FUW-EGFP-PURO

    Plasmid
    #251732
    Purpose
    Overexpression construct expressing EGFP-PURO and ORF-BC
    Depositor
    Andrea Califano
    Insert
    EGFP
    Use
    Lentiviral
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    251732-plasmid-map-sequence-id-501476

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  12. pCR8GW-HA-Wnk

    Plasmid
    #203967
    Purpose
    HA-Wnk basic construct
    Depositor
    Andreas Jenny
    Insert
    Wnk (most similar to RO variant) (Wnk Fly)
    Use
    Unspecified
    Tags
    HA
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203967-plasmid-map-sequence-id-403297

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  13. pENTR3cDual-Fray

    Plasmid
    #203977
    Purpose
    Enrty clone for Gateway cloning
    Depositor
    Andreas Jenny
    Insert
    Frayed (fray Fly)
    Use
    Unspecified
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203977-plasmid-map-sequence-id-403301

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  17. pOpen-ECOligA

    Plasmid
    #165564
    Purpose
    Connects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    E. coli DNA Ligase
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165564-plasmid-map-sequence-id-340043

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  21. pOpen-GBDpol (exo)

    Plasmid
    #165572
    Purpose
    Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165572-plasmid-map-sequence-id-340932

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  22. pET28a_LYN

    Plasmid
    #224238
    Purpose
    Expression of human LYN Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    lyn (LYN Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224238-plasmid-map-sequence-id-458419

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  23. pOpen-T4 g32

    Plasmid
    #165560
    Purpose
    A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T4 gene 32
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165560-plasmid-map-sequence-id-339953

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  24. pSP72 GSK3beta

    Plasmid
    #60125
    Purpose
    for transcription of GSK3beta
    Depositor
    Andrew McMahon
    Insert
    Glycogen synthase kinase 3 beta (Gsk3b Mouse)
    Use
    Cloning
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60125-plasmid-map-sequence-id-307046

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  25. pOpen-BovDNTT

    Plasmid
    #165580
    Purpose
    Adds dT at the end of PCR blunted DNA fragments/vectors that can then be used in TOPO cloning; Addition of homopolymer tails to the 3' ends of DNA; Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP); TUNEL assay (in situ localization of apoptosis); TdT dependent PCR
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bovine DNTT
    Use
    Synthetic Biology
    Available Since
    July 28, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165580-plasmid-map-sequence-id-338558

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  30. pREG-SRTF1

    Plasmid
    #246680
    Purpose
    Expresses the SRTF1 transcription factor in E. coli
    Depositor
    Andrew Ellington
    Insert
    Steroid responsive transcription factor 1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    246680-plasmid-map-sequence-id-493046

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  34. pET28a_HCK

    Plasmid
    #224239
    Purpose
    Expression of human HCK Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    hck (HCK Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    224239-plasmid-map-sequence-id-459258

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  35. pAMW-Wnk

    Plasmid
    #203978
    Purpose
    Myc6 Wnk under actin promoter for cell culture
    Depositor
    Andreas Jenny
    Insert
    Wnk (Wnk Fly)
    Tags
    6xMyc
    Expression
    Insect
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203978-plasmid-map-sequence-id-403206

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  36. pCVL RipTAL(Nd154C63) SFFV Xba-Sal linker

    Plasmid
    #50627
    Purpose
    pRipTAL plasmid compatible with Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50627-plasmid-map-sequence-id-79594

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  37. pCVL TAL(Nd154C63) T7 Age-Xho/Xba-Sal linkers

    Plasmid
    #50624
    Purpose
    pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Mrna production
    Promoter
    T7
    Available Since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50624-plasmid-map-sequence-id-75688

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  41. pAAV-BD-hSynTet-TRE-DIO-mNeonGreen-E2

    Plasmid
    #242784
    Purpose
    Bidirectional AAV transfer plasmid expressing hSyn-tTA and TRE-FLEX-mNeonGreen-E2 in the other direction.
    Depositor
    Andrew Payne
    Inserts
    mNeonGreen
    tTA-Advanced
    Use
    AAV
    Tags
    E2
    Promoter
    TRE and hSyn
    Available Since
    Sept. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    242784-plasmid-map-sequence-id-486990

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  42. pAAV-BD-hSynTet-TRE-DIO-mNeonGreen-HA

    Plasmid
    #242786
    Purpose
    Bidirectional AAV transfer plasmid expressing hSyn-tTA and TRE-FLEX-mNeonGreen-HA in the other direction.
    Depositor
    Andrew Payne
    Inserts
    mNeonGreen
    tTA-Advanced
    Use
    AAV
    Tags
    HA
    Promoter
    TRE and hSyn
    Available Since
    Sept. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    242786-plasmid-map-sequence-id-486974

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  43. pAAV-BD-hSynTet-TRE-DIO-mNeonGreen-S1

    Plasmid
    #242793
    Purpose
    Bidirectional AAV transfer plasmid expressing hSyn-tTA and TRE-FLEX-mNeonGreen-S1 in the other direction.
    Depositor
    Andrew Payne
    Inserts
    mNeonGreen
    tTA-Advanced
    Use
    AAV
    Tags
    S1
    Promoter
    TRE and hSyn
    Available Since
    Sept. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    242793-plasmid-map-sequence-id-486968

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  44. pAAV-BD-hSynTet-TRE-DIO-mNeonGreen-T7

    Plasmid
    #242796
    Purpose
    Bidirectional AAV transfer plasmid expressing hSyn-tTA and TRE-FLEX-mNeonGreen-T7 in the other direction.
    Depositor
    Andrew Payne
    Inserts
    mNeonGreen
    tTA-Advanced
    Use
    AAV
    Tags
    T7
    Promoter
    TRE and hSyn
    Available Since
    Sept. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    242796-plasmid-map-sequence-id-486965

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  46. pGEX6P1-DEST

    Plasmid
    #119749
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119749-plasmid-map-sequence-id-233993

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  47. pOpen-RNAse A

    Plasmid
    #165565
    Purpose
    Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    RNAse A
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165565-plasmid-map-sequence-id-340093

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  48. pGEX6P1-N-HA

    Plasmid
    #119756
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and HA
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119756-plasmid-map-sequence-id-233665

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  49. pCambia-loxKan

    Plasmid
    #199590
    Purpose
    basic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibility
    Depositor
    Andreas Stuermer
    Insert
    FRT-lox66-NosP-KanR-NosT-lox71-FRT
    Use
    Cre/Lox
    Expression
    Plant
    Promoter
    Nos
    Available Since
    July 5, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199590-plasmid-map-sequence-id-399425

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  50. pOpen-ETSSB

    Plasmid
    #165549
    Purpose
    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Extreme Thermostable Single-Stranded DNA Binding Protein
    Use
    Synthetic Biology
    Available Since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165549-plasmid-map-sequence-id-339430

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Showing: 4101 - 4150 of 5577 results