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We narrowed to 5,049 results for: golden gate

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Showing: 4101 - 4150 of 5049 results

  3. pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)

    Plasmid
    #181787
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181787-plasmid-map-sequence-id-354706

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  8. pTU2S-b (colE1 origin)

    Plasmid
    #74092
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    None
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74092-plasmid-map-sequence-id-141928

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  20. EC71139

    Plasmid
    #154062
    Purpose
    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    pZmUbi
    Use
    Synthetic Biology
    Mutation
    All BsaI, Esp3I and BpiI restriction sites were r…
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154062-plasmid-map-sequence-id-312952

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  24. pLaNGo11-3

    Plasmid
    #126054
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126054-plasmid-map-sequence-id-254921

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  25. pMiNGo11-1

    Plasmid
    #126056
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126056-plasmid-map-sequence-id-246974

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  28. pCVL TAL(Nd154C63) SFFV Xba-Sal linker

    Plasmid
    #50420
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Article
    Boissel et al Nucleic Acids Res. 2013 Nov 26.
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    SFFV
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50420-plasmid-map-sequence-id-75338

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  32. pRS479

    Plasmid
    #250493
    Purpose
    Regulatable CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 controlled by a tetO promoter, NEUT5L integration site and the sgRNA cloning site
    Depositor
    Rebecca Shapiro
    Article
    Wensing et al Nat Microbiol. 2026 May 11. doi: 10.1038
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    May 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    250493-plasmid-map-sequence-id-499651

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  33. pYSD741

    Plasmid
    #253052
    Purpose
    Encodes MFapp8 Sig Pep, Hydrophobin1 (HFB1), MYC-TEV-649-stalk surface display anchor in a yeast expression vector with pTEF2 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS origin
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Hydrophobin1 (HFB1)
    Expression
    Yeast
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253052-plasmid-map-sequence-id-508946

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  34. pYSD704

    Plasmid
    #253013
    Purpose
    Encodes Trichoderma reesei Hydrophobin1 (HFB1) as a Type 3b' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Hydrophobin1 (HFB1)
    Expression
    Bacterial
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253013-plasmid-map-sequence-id-508761

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  37. pYSD344

    Plasmid
    #253044
    Purpose
    Encodes 2xFLAG-tagged Aga2 with Protein A ZZ domain as a C-terminal fusion in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Aga2 Protein A
    Expression
    Yeast
    Available Since
    May 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    253044-plasmid-map-sequence-id-508939

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  38. pYSD090

    Plasmid
    #252980
    Purpose
    Encodes MYC-tagged 649 stalk display anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    MYC-tagged 649 stalk display anchor
    Expression
    Bacterial
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    252980-plasmid-map-sequence-id-508739

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  39. pYSD089

    Plasmid
    #252979
    Purpose
    Encodes 2xFLAG-tagged 649 stalk display anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    FLAG-tagged 649 stalk display anchor
    Expression
    Bacterial
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    252979-plasmid-map-sequence-id-508738

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  40. pYSD078

    Plasmid
    #252975
    Purpose
    Encodes a MoClo Yeast Toolkit (YTK) Type 3 GFP dropout part that can be used to make a YTK GFP dropout vector containing all part types except Type 3
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Protein A
    Expression
    Bacterial
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    252975-plasmid-map-sequence-id-508688

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  41. pYSD890

    Plasmid
    #253016
    Purpose
    Encodes S. cerevisiae Met15 as a Type 6 part to be used in the MoClo yeast toolkit (YTK) and yeast secretion/display (YSD2.0) extension of the MoClo YTK
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    MET15
    Expression
    Bacterial
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253016-plasmid-map-sequence-id-508685

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  42. pYSD086

    Plasmid
    #252976
    Purpose
    Encodes FLAG-tagged S. cerevisiae Sed1p display anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    FLAG-tagged Sed1
    Expression
    Bacterial
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    252976-plasmid-map-sequence-id-508653

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  45. pYSD056

    Plasmid
    #252969
    Purpose
    Encodes S. cerevisiae HA Tag-Aga2-linker as a Type 3a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Aga2
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252969-plasmid-map-sequence-id-507371

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  46. pYSD057

    Plasmid
    #252970
    Purpose
    Encodes S. cerevisiae 2xFLAG-Tag-Aga2-linker as a Type 3a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Aga2
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252970-plasmid-map-sequence-id-507372

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  47. pYSD054

    Plasmid
    #252967
    Purpose
    Encodes S. cerevisiae MYC Tag-Aga2-linker as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Aga2
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252967-plasmid-map-sequence-id-507369

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  48. pYSD055

    Plasmid
    #252968
    Purpose
    Encodes S. cerevisiae Aga2-(NoTag)-linker as a Type 3a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Aga2
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252968-plasmid-map-sequence-id-507370

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  49. pYSD053

    Plasmid
    #252966
    Purpose
    Encodes S. cerevisiae 2xFLAG-Tag-Aga2-linker as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Aga2
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252966-plasmid-map-sequence-id-507367

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  50. pYSD088

    Plasmid
    #252978
    Purpose
    Encodes 2xFLAG-Tagged S. cerevisiae Aga2p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit for N-terminal fusion
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    FLAG-tagged Aga2
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252978-plasmid-map-sequence-id-507358

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Showing: 4101 - 4150 of 5049 results