We narrowed to 31,103 results for: PLE

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pLenti-PGK-RAP1A-WT-puro

Plasmid

#156166

Purpose

Lentiviral vector for expression of RAP1A-WT, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLSNG-P2A-Puro

Plasmid

#136900

Purpose

Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSNG

Use

Lentiviral

Tags

FLAG-NLS and NLS

Mutation

L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

Promoter

EF1s

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet-MKK6(DD)-Puro

Plasmid

#86094

Purpose

Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

Depositor

Cindy Miranti

Article

Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

Insert

MKK6 (MAP2K6 Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Mutation

Serine 207 and Threonine 211 both changed to Aspa…

Promoter

CMV/TetO

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti sgRNA(MS2) mCherry

Plasmid

#171053

Purpose

Expression of Vp64-dCas9 activation system

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

sgRNA(MS2) mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA(lib)-puro

Plasmid

#119976

Purpose

Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Wensheng Wei

Article

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CD74-ROS1 G2032R

Plasmid

#183814

Purpose

Express CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R mutation in mammalian cells

Depositor

Aaron Hata

Article

Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

Inserts

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
CD74 molecule (CD74 Human)

Use

Retroviral

Tags

The fusion protein of CD74 exon 1-6 and ROS1 exon…

Expression

Mammalian

Mutation

G2032R

Promoter

CMV

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Hygro DEST (w17-1)

Plasmid

#17295

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-xF2X-P2A-Puro

Plasmid

#110873

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS-2X(3.7)

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DHFR.cHSF1

Plasmid

#134739

Purpose

Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202

Promoter

CMV

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-PtenC124S-Puro

Plasmid

#135677

Purpose

Constitutive expression (cMV promoter) of C124S mutant Pten cDNA, Puromycin selection

Depositor

Florian Karreth

Insert

Pten (Pten Mouse)

Use

Lentiviral

Mutation

C124S

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Neo empty (w36-1)

Plasmid

#17478

Purpose

3rd gen lentiviral RNAi empty control vector, 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hCRY1 c-term

Plasmid

#179453

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human CRY1

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST P38KTRDronpa

Plasmid

#205763

Purpose

Expression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

P38 KTR Dronpa

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-DNFGFR1-HA

Plasmid

#130887

Purpose

Expresses dominant negative mutant mouse FGFR1

Depositor

Gian-Paolo Dotto

Article

Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

Insert

Fgfr1 (Fgfr1 Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

Dominant Negative receptor lacking cytosolic cata…

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-xFNLS-P2A-Puro

Plasmid

#110872

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS(3.7)

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-DENV2A-GFP-puro

Plasmid

#140089

Purpose

Fluorescence activatable reporter of DENV-2 NS2B-NS3 protease activity

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

Insert

DENV2A-GFP

Use

Lentiviral; Low expression

Tags

6XHis tag and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-CD90.2-Rluc_miR

Plasmid

#163360

Purpose

Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a CD90.2 surface marker.

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

CD90.2 (Thy1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

EF1

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-Archer1-EGFP

Plasmid

#60423

Purpose

Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors changes in membrane voltage.

Depositor

Viviana Gradinaru

Article

Flytzanis et al Nat Commun. 2014 Sep 15;5:4894. doi: 10.

Insert

Archer1-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pLEX-Frb:Akt2-IRES-Hygromycin

Plasmid

#120713

Purpose

Expresses Frb:Akt2 fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

AKT2 (AKT2 Human)

Use

Lentiviral

Tags

FRB(T2098L)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet-GBP1-C589A

Plasmid

#138090

Purpose

Lentiviral backbone for inducible expression of human GBP1 missing the reactive cysteine of the CaaX-box (GBP1-C589A)

Depositor

Eva Frickel

Article

Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

Insert

human GBP1 (GBP1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

cysteine 589 to alanine (C589A)

Promoter

minimal CMV promoter with heptamerized tet‐operat…

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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