We narrowed to 4,416 results for: BRE
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Plasmid#155127PurposeDonor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld methodDepositorInsertP2A-QFGal4
UseCRISPR and Synthetic BiologyAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαi*-BERKY3
Plasmid#158428PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseLentiviralTagsMycExpressionMammalianPromoterhSynapsinAvailable SinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-VinculinI997A_TurboID_Venus
Plasmid#200274PurposeExpressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter VenusDepositorInsertVinculinI997A_TurboID_Venus
ExpressionMammalianMutationchange isoleucine to alanine at 997 of vinculinPromoterT7Available SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαq*-BERKY1
Plasmid#158430PurposeBRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-GRK2(RH)
UseLentiviralExpressionMammalianPromoterhSynapsinAvailable SinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)
Plasmid#117908PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
p47phox-PX (1-122)
Plasmid#119124PurposeBacterial expression of human phox homology (PX) domain, p47phox-PX (1-122)DepositorInsertp47phox-PX (1-122)
TagsGSTExpressionBacterialPromotertacAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαi*-BERKY1
Plasmid#158427PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-KB1753-myc
UseLentiviralTagsmycExpressionMammalianPromoterhSynapsinAvailable SinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
mPLD1-PX (71-210)
Plasmid#119127PurposeBacterial expression of human phox homology (PX) domain, mPLD1-PX (71-210)DepositorInsertmPLD1-PX (71-210)
TagsGSTExpressionBacterialPromotertacAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)
Plasmid#117911PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
TagsHis-tag and Strep-tagExpressionBacterialMutationR44A & H45Q on eDHFRPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-IDS_DF_C2c_attB_rev
Plasmid#182151PurposeTo insert attB-rev attachment site at IDS in human cells via twinPEDepositorInsertIDS-C2c-attBrev pegRNA
ExpressionMammalianPromoterhU6Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-2 (C50 29 nM)
Plasmid#117909PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
TagsHis-tag and Strep-tagExpressionBacterialMutationR98A on eDHFRPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHD Btl-pYtag(Scarlet) ScarlessDsRed
Plasmid#244937PurposeVector for tagging Breathless (Btl) with Scarlet-based pYtag biosensor. DsRed marker.DepositorInsertmScarlet-tagged pYtag system (3xITAM P2A mScarlet-ZtSH2)
UseEndogenous taggingAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
hVAMP3(1-77) S44A
Plasmid#206029PurposeExpresses the SNARE motif of human VAMP3 mutant S44A in bacteriaDepositorInsertVesicle-associated membrane protein 3 (VAMP3 Human)
Tagshis-tagExpressionBacterialMutationchanges serine 44 to alaninePromoterT7Available SinceOct. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
hVAMP3(1-77) S44E
Plasmid#206030PurposeExpresses the SNARE motif of human VAMP3 mutant S44E in bacteriaDepositorInsertVesicle-associated membrane protein 3 (VAMP3 Human)
Tagshis-tagExpressionBacterialMutationchanges serine 44 to glutamic acidPromoterT7Available SinceOct. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-BbsI-Btl
Plasmid#244934PurposeCRISPR gRNA targeting the C-terminal end of Breathless for cleavage.DepositorInsertgRNA targeting Btl C terminus
UseCRISPRAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEmal10H – MBP-EWS_300-447 (RGG1+RRM)
Plasmid#238369PurposeExpresses the RGG1-RRM (300-447) region from the RNA-binding protein EWSDepositorInsertEWSR1 (EWSR1 Human)
Tags10xHis-MBPExpressionBacterialMutationdeleted amino acids 1-299, 448-638PromoterT7Available SinceSept. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEmal10H – MBP-EWS_549-638 (RGG3)
Plasmid#238370PurposeExpresses the RGG3 (549-638) region from the RNA-binding protein EWSDepositorInsertEWSR1 (EWSR1 Human)
Tags10xHis-MBPExpressionBacterialMutationdeleted amino acids 1-548PromoterT7Available SinceSept. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF1b-His8-TEV-PI31-1-151
Plasmid#202593PurposeBacterial expression of HsPI31-1-151DepositorAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
mPLD2-PX (62-192)
Plasmid#119128PurposeBacterial expression of human phox homology (PX) domain, mPLD2-PX (62-192)DepositorInsertmPLD2-PX (62-192)
TagsGSTExpressionBacterialPromotertacAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Trf2
Plasmid#125159PurposeGateway entry cloneDepositorInsertTrf2 (Trf2 Fly)
UseGateway entry vectorAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Theo-LUCID
Plasmid#119048PurposePlasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Theo-LUCID_part1
Fab_Theo-LUCID_part2
TagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Quin-LUCID
Plasmid#119049PurposePlasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Quin-LUCID_part1
Fab_Quin-LUCID_part2
TagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_MTX-LUCID
Plasmid#119047PurposePlasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_MTX-LUCID_part1
Fab_MTX-LUCID_part2
TagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
SNX26-PX (56-172)
Plasmid#119105PurposeBacterial expression of human phox homology (PX) domain, SNX26-PX (56-172)DepositorInsertSNX26-PX (56-172)
TagsGSTExpressionBacterialPromotertacAvailabilityAcademic Institutions and Nonprofits only -
SNX28-PX (1-124)
Plasmid#119107PurposeBacterial expression of human phox homology (PX) domain, SNX28-PX (1-124)DepositorInsertSNX28-PX (1-124)
TagsGSTExpressionBacterialPromotertacAvailabilityAcademic Institutions and Nonprofits only -
SNX34-PX (1-130)
Plasmid#119113PurposeBacterial expression of human phox homology (PX) domain, SNX34-PX (1-130)DepositorInsertSNX34-PX (1-130)
TagsGSTExpressionBacterialPromotertacAvailabilityAcademic Institutions and Nonprofits only -
IRAS-PX (13-124)
Plasmid#119118PurposeBacterial expression of human phox homology (PX) domain, IRAS-PX (13-124)DepositorInsertIRAS-PX (13-124)
TagsGSTExpressionBacterialPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pEND-350_Cacnes_ori
Plasmid#225605PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ash2L full
Plasmid#15548DepositorInsertAsh2L (ASH2L Human)
ExpressionMammalianMutationR622H (Please see depositor comments below)Available SinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-q ONE-GO
Plasmid#189738PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHTNW
Plasmid#136403PurposeExpression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.DepositorTypeEmpty backboneTagsHaloTagExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP8
Plasmid#42311DepositorAvailable SinceMarch 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i1 ONE-GO
Plasmid#189735PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEND-349_Cacnes_ori
Plasmid#225604PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3 ONE-GO
Plasmid#189737PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO
Plasmid#204341PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-i3/Gbetagamma ONE-GO
UseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJ609-dCas9-KR-puro
Plasmid#158478PurposeExpress nuclease dead Cas9 fused to KillerRed chromophore in mammalian cellsDepositorInsertdCas9-KR
UseCRISPRTagsKillerRedExpressionMammalianMutationCas9m4 (D10A+D839A+H840A+N863A)PromoterCMVAvailable SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i2 ONE-GO
Plasmid#189736PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJR017
Plasmid#198835PurposeIn vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.DepositorInsert15xUAS::TeTX-SL2-mKate::let-858 3'UTR
ExpressionWormAvailable SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA
Plasmid#155125PurposeTol2 vector containing Cre-inducible QFGal4-switchDepositorInsertQFGal4-switch
UseCre/Lox; Zebrafish expressionPromoterUbiAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNLF1W
Plasmid#136404PurposeExpression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.DepositorTypeEmpty backboneUseLuciferaseTagsNanoLuc luciferaseExpressionMammalianPromoterCMVAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
L2125-FRB-GPA-NLuc-CyOFP1
Plasmid#222885PurposePositive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.DepositorInsertFRB-GPA-20GS-NanoLuc-10GS-CyOFP1
TagsMycExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO
Plasmid#204340PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-s/Gbetagamma ONE-GO
UseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
ECFP-Bcr
Plasmid#36415DepositorAvailable SinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
SNX10-PX (1-201)
Plasmid#119091PurposeBacterial expression of human phox homology (PX) domain, SNX10-PX (1-201)DepositorAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Actin Binding Domain Tension Sensor
Plasmid#215368PurposeExpresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.DepositorInsertActin Binding Domain Tension Sensor
ExpressionMammalianPromoterCMVAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUAS5x:GFPNLS-SV40pA
Plasmid#155122PurposeTol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUAS5x:GFPNLS
UseZebrafish expressionPromoterQUAS5x-E1bAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro GSK3 beta (S9A)
Plasmid#14128DepositorInsertGSK3 beta (S9A) (GSK3B Human)
UseRetroviralTagsV5 tagExpressionMammalianMutationS9A. Mutant version of GSK3 that lacks the inhibi…Available SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
SNX29-PX (269-391)
Plasmid#119108PurposeBacterial expression of human phox homology (PX) domain, SNX29-PX (269-391)DepositorInsertSNX29-PX (269-391)
TagsGSTExpressionBacterialPromotertacAvailable SinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUASM1:GFPNLS-SV40pA
Plasmid#155123PurposeTol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUASM1:GFPNLS
UseZebrafish expressionPromoterQUASM1-E1bAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only