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We narrowed to 50,495 results for: ang

Showing: 4151 - 4200 of 50495 results

  1. pEJS1354 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-1 CMV-BFP

    Plasmid
    #226113
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-1-5p Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226113-plasmid-map-sequence-id-448319

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  2. pEJS1355 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP

    Plasmid
    #226114
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion without microRNA target sites in the 3' UTR as a control reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Use
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226114-plasmid-map-sequence-id-448323

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  3. pEJS1352 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-22 CMV-BFP

    Plasmid
    #226111
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-22 Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226111-plasmid-map-sequence-id-448317

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  7. pYPQ265E7

    Plasmid
    #213470
    Purpose
    Gateway entry clone (attL1 & attR5) TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing
    Depositor
    Yiping Qi
    Article
    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:
    Insert
    TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI
    Use
    CRISPR; Gateway compatible tada-cdd-32aa linker-z…
    Tags
    No
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213470-plasmid-map-sequence-id-426727

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  8. pYPQ265E8

    Plasmid
    #213471
    Purpose
    Gateway entry clone (attL1 & attR5) TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing
    Depositor
    Yiping Qi
    Article
    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:
    Insert
    TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI
    Use
    CRISPR; Gateway compatible tada-cdd_v106w-32aa li…
    Tags
    No
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213471-plasmid-map-sequence-id-426730

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  13. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid
    #184774
    Purpose
    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
    Depositor
    Zhiping Xie
    Article
    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10
    Insert
    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Mouse, Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    184774-plasmid-map-sequence-id-382498

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  14. pCAT079

    Plasmid
    #180509
    Purpose
    Plasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    DpbCasX
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available Since
    June 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    180509-plasmid-map-sequence-id-364076

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  20. pBT3038

    Plasmid
    #122545
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal related organelle localized gene (glo-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_glo-1::CemOrange2 (glo-1 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122545-plasmid-map-sequence-id-238133

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  21. pBT3043

    Plasmid
    #122546
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian LC3 (sqst-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::lgg-1 (lgg-1 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122546-plasmid-map-sequence-id-238140

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  28. pAAV-TRE-DIO-ChR2-EYFP

    Plasmid
    #183765
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The ChR2-EYFP expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene -teto promoter-flex/dio-ChR2-EYFP
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    ChR2-EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183765-plasmid-map-sequence-id-361682

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  31. pAAV-TRE-DIO-hM4Di-mCherry

    Plasmid
    #182532
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The hM4Di receptor expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene - hSyn promoter-tet-flex-hM4Di-cherry
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    hM4Di-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182532-plasmid-map-sequence-id-361681

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  33. ACBE-Q

    Plasmid
    #204608
    Purpose
    Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …
    Promoter
    Available Since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204608-plasmid-map-sequence-id-404717

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  36. pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG, I253A, H310A, H435A

    Plasmid
    #222855
    Purpose
    Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc lacking FcRn binding
    Depositor
    Andrew Kruse
    Article
    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038
    Insert
    AT118-L
    Use
    Tags
    IgG1_Fc
    Expression
    Mammalian
    Mutation
    L234A, L235A, P329G, H435A, I253A, H310A
    Promoter
    Available Since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    222855-plasmid-map-sequence-id-445010

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  45. pCAT100

    Plasmid
    #180512
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180512-plasmid-map-sequence-id-359730

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  46. pAC94-pmax-dCas9VP160-2A-puro

    Plasmid
    #48226
    Purpose
    dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
    Use
    CRISPR
    Tags
    2A-puro, HA Tag, and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available Since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48226-plasmid-map-sequence-id-69514

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  47. pCAT527

    Plasmid
    #180514
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180514-plasmid-map-sequence-id-359727

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  48. pAAV-TRE-DIO-Casp3-TEV

    Plasmid
    #183766
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183766-plasmid-map-sequence-id-361683

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Showing: 4151 - 4200 of 50495 results