We narrowed to 2,378 results for: 1186

1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pOCC61

   Plasmid

   #118877

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
   

   Tags

   mGFP, HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1a-tetR-T2A-iRFP

   Plasmid

   #176028

   Purpose

   construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   tetR
   iRFP
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDECKO_TFRC_B

   Plasmid

   #72626

   Purpose

   Expresses two gRNAs targeting the TFRC promoter

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward TFRC
   

   Expression

   Mammalian

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPMVAd36

   Plasmid

   #98302

   Purpose

   Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

   Expression

   Yeast

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQTEV-GYG

   Plasmid

   #31324

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   glycogenin (GYG1 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRW006-SM-destination-pro35S-Cas9

   Plasmid

   #104439

   Purpose

   Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial and Plant

   Promoter

   35S

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDECKO_Malat1_B

   Plasmid

   #72621

   Purpose

   Expresses two gRNAs targeting MALAT1 promoter

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward Malat1
   

   Expression

   Mammalian

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV_Mock

   Plasmid

   #223565

   Purpose

   The plasmid is expressed in mammalian cells as a control.

   Depositor

   Hyun Woo Park

   Article

   Shin et al Mol Cancer. 2023 Nov 6;22(1):177. doi: 1

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-CAG-NCreIntN-EF1a-mTagBFP2

   Plasmid

   #160507

   Purpose

   Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   NCreIntN
   

   Use

   Cre/Lox and Lentiviral

   Tags

   mTagBFP2

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-LbCas12a-Plus-mCherry

    Plasmid

    #191230

    Purpose

    To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    LbCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Mutation

    D156R, R883K, R887A

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOCC267

    Plasmid

    #118887

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; HALO tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA

    Plasmid

    #97010

    Purpose

    Rosa26 targeting vector for FLP-responsive cassette

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Expression

    Mammalian

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTYF-1xSYN-EGFP

    Plasmid

    #19973

    Depositor

    Sergey Kasparov

    Article

    Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

    Insert

    SYN-EGFP-WPRE (SYN1 Human, jellyfish)
    

    Expression

    Mammalian

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-HA-dSpCas9

    Plasmid

    #92112

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9
    

    Use

    CRISPR

    Tags

    HA tag and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PliaI

    Plasmid

    #55177

    Purpose

    Bacitracin-inducible promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    liaI promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    liaI

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPMVAugw

    Plasmid

    #98300

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    TEFM1EfmvaE>TEBS1
    

    Expression

    Yeast

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pILGFP97

    Plasmid

    #83551

    Purpose

    Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    CYC1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    CYC1

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ230-RV

    Plasmid

    #188544

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RV
    

    Use

    CRISPR; Gateway compatible lbcas12a-rv entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, R182V

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PliaG

    Plasmid

    #55174

    Purpose

    Constitutive promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    LiaG promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQTEV-PRKRA

    Plasmid

    #31571

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge