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Showing: 1661 - 1680 of 2496 results

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  2. ePPEplus

    Plasmid
    #205241
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Article
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205241-plasmid-map-sequence-id-408831

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  10. pET28a(+)::NL3F10H

    Plasmid
    #141290
    Purpose
    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3) and His (x10)
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available Since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141290-plasmid-map-sequence-id-324909

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  11. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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  15. pOCC102

    Plasmid
    #118883
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118883-plasmid-map-sequence-id-232315

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  19. pTrEno

    Plasmid
    #115474
    Purpose
    Eno-promoter driven expression vector for protein production in Trichoderma reesei
    Depositor
    Steve Decker
    Article
    Linger et al Biotechnol Biofuels. 2015 Mar 18;8:45. d
    Insert
    Cel7A
    Use
    Unspecified
    Mutation
    Introns have been removed
    Promoter
    Trichoderma reesei Enolase (Eno) promoter
    Available Since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    115474-plasmid-map-sequence-id-225594

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Showing: 1661 - 1680 of 2496 results