We narrowed to 1,763 results for: MB;

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pmiRFP-H2B-C1

Plasmid

#108411

Purpose

Histone 2B fused to monomeric near-infrared fluorescent protein miRFP

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP-H2B (H2BC21 Human)

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP

Plasmid

#108410

Purpose

Monomeric near-infrared fluorescent protein miRFP in bacterial expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Expression

Bacterial

Promoter

araB

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Soma-jGCaMP8f

Plasmid

#182943

Purpose

Cell body-targeted jGCaMP8f, under synapsin promoter.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-jGCaMP8f

Use

AAV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pT2-shATRX-GFP

Plasmid

#124259

Purpose

Expresses shRNA targeting ATRX with a GFP reporter which is driven by the SV40 promoter. Construct has inverted repeats to be used in Sleeping beauty system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

shATRX

Expression

Mammalian

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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PGK-LAMP1-3xFLAG-APEX2

Plasmid

#253243

Purpose

Express LAMP1 fused to APEX2 peroxidase to map the spatial organization of the lysosomal membrane and its associated proteins. LAMP1 is also fused to 3xFlag for IF or WB detection.

Depositor

May Malicdan

Article

Serra-Vinardell et al Cell Mol Life Sci. 2023 Jan 28;80(2):53.

Insert

LAMP1 (LAMP1 Human)

Use

Lentiviral

Tags

3xFLAG-APEX2

Promoter

PGK

Available Since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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PB-AlfaTag

Plasmid

#253248

Purpose

Empty plasmid for cloning or as control for PB-LYST-AlfaTag (Plasmid #253252) and PB-AlfaTag-MYO5A (Plasmid #253250)

Depositor

May Malicdan

Article

Serra-Vinardell et al Cell Mol Life Sci. 2023 Jan 28;80(2):53.

Type

Empty backbone

Use

Piggybac transposon

Tags

AlfaTag

Expression

Mammalian

Available Since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-ChRmine-ST-P2A-H2B-mRuby3

Plasmid

#227779

Purpose

Cation channelrhodopsin ChRmine targeted to the neuronal soma and proximal dendrites in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

DIO-ChRmine-ST-P2A-H2B-mRuby3

Use

AAV

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7RE ∆238-245

Plasmid

#139318

Purpose

GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

AO7 cloned into the first multiple cloning site o…

Promoter

T7 promoter-1

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pJS697

Plasmid

#168778

Purpose

Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Type

Empty backbone

Tags

Cmyc and HA

Expression

Yeast

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCas59

Plasmid

#82396

Purpose

sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCas45

Plasmid

#82390

Purpose

sgRNA targeting YFP with additional bases 5' end like pCas34, but from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PJ23119

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas57

Plasmid

#82395

Purpose

sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas56

Plasmid

#82394

Purpose

sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas55

Plasmid

#82393

Purpose

sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas52

Plasmid

#82392

Purpose

sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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MT583

Plasmid

#63114

Purpose

The gDNA construct is designed for arabidopsis ecotype Est-1 ACD6 expression in other genetic backgrounds.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

ACD6 (Accelerated Cell Death 6)

Expression

Plant

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

Plasmid

#209086

Purpose

Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)
BirA

Tags

AviTag

Expression

Mammalian

Mutation

human codon optimized

Promoter

TRE4G and hSyn

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)

Plasmid

#209085

Purpose

Ngn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expression

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)
BirA

Expression

Mammalian

Mutation

human codon optimized

Promoter

TRE4G and hSyn

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLY59_TRAC-LHA-pAAV-EFS-CD22BBz-PRODH2(stop)-TRAC-RHA(3166mut)

Plasmid

#192189

Purpose

CD22 CAR AAV vector PRODH2(StopCtrl) (pLY059)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

CD22 CAR AAV vector PRODH2(StopCtrl) (pLY059) (CD22 Synthetic)

Use

AAV; Mammalian expression

Mutation

NA

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Archon1-KGC-EGFP

Plasmid

#108418

Purpose

AAV production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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