We narrowed to 5,965 results for: crispr cas9 expression plasmids

1. pTBPL1.1.0-gDNA

   Plasmid

   #112412

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TBPL1

   Depositor

   Kevin White

   Insert

   TBPL1 (TBPL1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMAD3.1.0-gDNA

   Plasmid

   #112407

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SMAD3

   Depositor

   Kevin White

   Insert

   SMAD3 (SMAD3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57-Simple-gRNA backbone

   Plasmid

   #51306

   Purpose

   gRNA expression plasmid

   Depositor

   Yonglong Chen

   Article

   Guo et al Development. 2014 Jan 8.

   Type

   Empty backbone

   Use

   CRISPR; Xenopus expression

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMLS291

   Plasmid

   #73724

   Purpose

   SapTrap mCherry + Cbr-unc-119 donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   mCherry + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMLS252

   Plasmid

   #73720

   Purpose

   SapTrap GFP + Cbr-unc-119 donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   GFP + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMLS286

   Plasmid

   #73723

   Purpose

   Saptrap tagRFP + Cbr-unc-119 donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   tagRFP + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMLS271

   Plasmid

   #73722

   Purpose

   SapTrap SNAPtag + Cbr-unc-119 donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Snaptag + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMLS254

   Plasmid

   #73721

   Purpose

   SapTrap Halotag + Cbr-unc-119 donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Halotag + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONOR 6colors

   Plasmid

   #229974

   Purpose

   bigMamAct assembled plasmid for 6colors cellular labelling

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. psLIB CMV

    Plasmid

    #229953

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. Has empty CMV-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. psLIB U6

    Plasmid

    #229956

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. psLIB EF1alpha

    Plasmid

    #229954

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. psLIB TRE

    Plasmid

    #229955

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. Has empty Tet-responsive element (TRE)-driven cassette; users should clone gene of interest into shuttle plasmid prior to Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMLS292

    Plasmid

    #73725

    Purpose

    SapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    2xNLS-mCherry + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-U6-sgRNA-CMV-GFP

    Plasmid

    #85451

    Purpose

    Express sgRNA in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pCMV-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pCMV-EGFP

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. HCP8

    Plasmid

    #166110

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5

    Depositor

    Matthias Heinemann

    Insert

    Whi5-sg106 (WHI5 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCfB8622

    Plasmid

    #126912

    Purpose

    Plasmid containing gRNA expression cassette targeting ADE2 in Saccharomyces cerevisiae

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    ADE2 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMpGE_En01

    Plasmid

    #71534

    Purpose

    Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Takayuki Kohchi, Ikuko Hara-Nishimura

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCJ1023

    Plasmid

    #228763

    Purpose

    Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFD3-ebony

    Plasmid

    #83380

    Purpose

    Drosophila gRNA expression plasmid targets ebony

    Depositor

    Richard Padgett

    Article

    Kane et al G3 (Bethesda). 2017 Jan 5;7(1):87-93. do

    Insert

    ebony (e Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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