We narrowed to 6,001 results for: crispr cas9 expression plasmids

1. sgiRFP1

   Plasmid

   #113972

   Purpose

   Single short guide RNA targeting GATCGAGTTCGAGCCTGCGG in iRFP670 sequence

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   iRFP670 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgiRFP2

   Plasmid

   #113973

   Purpose

   Single short guide RNA targeting GCGCGTTCTTTGGACGCGA in iRFP670 sequence

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   iRFP670 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgiRFP3

   Plasmid

   #113974

   Purpose

   Single short guide RNA targeting CGTGATGTTGTACCGCTTC in iRFP670 sequence

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   iRFP670 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLX-sgRNA

   Plasmid

   #50662

   Purpose

   Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

   Depositor

   David Sabatini, Eric Lander

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   sgAAVS1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

   Plasmid

   #68357

   Purpose

   The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Inserts

   5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
   FlpO recombinase
   

   Use

   CRISPR

   Tags

   mTurquoise2 (BFP)

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and synthetic Probasin ARRx2

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNR4A1.1.0-gDNA

   Plasmid

   #112391

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1

   Depositor

   Kevin White

   Insert

   NR4A1 (NR4A1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTEAD1.1.0-gDNA

   Plasmid

   #112425

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TEAD1

   Depositor

   Kevin White

   Insert

   TEAD1 (TEAD1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTFAP2B.1.0-gDNA

   Plasmid

   #113791

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2B

   Depositor

   Kevin White

   Insert

   TFAP2B (TFAP2B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZNF423.1.0-gDNA

   Plasmid

   #113790

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF423

   Depositor

   Kevin White

   Insert

   ZNF423 (ZNF423 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pREST.1.0-gDNA

    Plasmid

    #113774

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor REST

    Depositor

    Kevin White

    Insert

    REST (REST Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZNF462.1.0-gDNA

    Plasmid

    #112476

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF462

    Depositor

    Kevin White

    Insert

    ZNF462 (ZNF462 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTBPL1.1.0-gDNA

    Plasmid

    #112412

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TBPL1

    Depositor

    Kevin White

    Insert

    TBPL1 (TBPL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSMAD3.1.0-gDNA

    Plasmid

    #112407

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SMAD3

    Depositor

    Kevin White

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC57-Simple-gRNA backbone

    Plasmid

    #51306

    Purpose

    gRNA expression plasmid

    Depositor

    Yonglong Chen

    Article

    Guo et al Development. 2014 Jan 8.

    Type

    Empty backbone

    Use

    CRISPR; Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-U6-sgRNA-CMV-GFP

    Plasmid

    #85451

    Purpose

    Express sgRNA in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pCMV-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pCMV-EGFP

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. psLIB CMV

    Plasmid

    #229953

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. Has empty CMV-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMLS291

    Plasmid

    #73724

    Purpose

    SapTrap mCherry + Cbr-unc-119 donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    mCherry + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMLS252

    Plasmid

    #73720

    Purpose

    SapTrap GFP + Cbr-unc-119 donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    GFP + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMLS286

    Plasmid

    #73723

    Purpose

    Saptrap tagRFP + Cbr-unc-119 donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    tagRFP + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMLS271

    Plasmid

    #73722

    Purpose

    SapTrap SNAPtag + Cbr-unc-119 donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    Snaptag + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge