166,289 results

1. pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya

   Plasmid

   #186980

   Purpose

   mammalian expression of Cluc-Gluc reporter

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   Cluc, Gluc
   

   Use

   Luciferase

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPOCK2_22-424_AviN_HisC

   Plasmid

   #195877

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SPOCK2 (SPOCK2 Human)
   

   Use

   Pfhmsp-avin-lic-c, baculovirus expression

   Promoter

   Polyhedrin

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSFDuet-1_DNAseI

   Plasmid

   #182408

   Purpose

   Bacterial expression of DNaseI

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   DNase I (DNASE1 Bovine)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pgk-ATG-frt

   Plasmid

   #20734

   Depositor

   Rudolf Jaenisch

   Article

   Beard et al Genesis. 2006 Jan . 44(1):23-8.

   Insert

   pgk-ATG-frt-SAdpA-tetO
   

   Use

   Mouse Targeting

   Available Since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CAG.GCaMP6f.WPRE.SV40

   Plasmid

   #100836

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV2, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   CAG

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLBH531_MBP-Cas14a1 expression

   Plasmid

   #112500

   Purpose

   Expresses MBP fused to Cas14a1

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14a1
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)

   Plasmid

   #85799

   Purpose

   donorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP

   Depositor

   Roland Friedel

   Article

   Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

   Insert

   PPP1R12C (PPP1R12C Human)
   

   Use

   Human targeting

   Mutation

   homology arms for knock-in into first intron

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pL1-pKPI-MtLAP1-Adh

   Plasmid

   #193520

   Purpose

   Anthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots

   Depositor

   Jeremy Murray

   Article

   Kumar et al New Phytol. 2022 Sep 20. doi: 10.1111/np

   Insert

   MtLAP1
   

   Expression

   Plant

   Promoter

   Medtr8g059790(KPI)

   Available Since

   June 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3β-FLAG-CBP-HA

   Plasmid

   #32908

   Depositor

   Tso-Pang Yao

   Article

   Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

   Insert

   CBP (Crebbp Mouse)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid

    #217800

    Purpose

    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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