164,577 results

1. pLenti-C-Myc-DDK-IRES-Puro HNRNPQ

   Plasmid

   #200729

   Purpose

   Lentiviral expression of Flag-Tagged human HNRNPQ

   Depositor

   Claudio Alarcón

   Insert

   HNRNPQ (SYNCRIP Human)
   

   Use

   Lentiviral

   Tags

   Myc-DDK from backbone plasmid

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PHR-GFP-FUSN-VP16

   Plasmid

   #183933

   Purpose

   Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   PHR-GFP-FUSN-VP16 (CRY2, UL48 Synthetic, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCaggs-mGold2t

   Plasmid

   #231764

   Purpose

   Expression of mGold2t in mammalian cells

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2t
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

   Promoter

   Actin promoter with CMV enhancer

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPD95_75

   Plasmid

   #1494

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Use

   Tags

   IVSA, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, IVSL…

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGWB452

   Plasmid

   #74834

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBXNH3CA_MBP

   Plasmid

   #132700

   Purpose

   expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   Maltose binding Protein
   

   Use

   Tags

   Avi and His

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDonor_Tn[himar1C9]

   Plasmid

   #213906

   Purpose

   Mariner transposon with ATc-inducible himar1C9 transposase

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with cam resistance and ATc-inducible himar1C9 transposase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. T7-VEE-mCherry

   Plasmid

   #193355

   Purpose

   Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   mCherry
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. iNos-eGFP

   Plasmid

   #207251

   Purpose

   Reporter for expression of eGFP under control of iNos promoter

   Depositor

   Michelle Farkas

   Article

   Mas-Rosario et al Front Oncol. 2023 Apr 5;13:1151384. doi:

   Insert

   iNos Promoter (Nos2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpCas9(BB)-2A-Puro (PX459)

    Plasmid

    #48139

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only