We narrowed to 29,779 results for: REP
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Plasmid#213863PurposeBinary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused sfGFP
UseTagsExpressionBacterial and PlantMutationPromoterAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FXR2P_P474S_P493N
Plasmid#198695PurposeExpression of human FXR2 protein in E. coliDepositorInsertFXR2P (FXR2 Human)
UseTagsMBPExpressionBacterialMutationChanged proline 474 to serine and proline 493 asp…PromoterT7Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rogi2-CMV-LAMB3-T2A-GFP-bGH
Plasmid#179865PurposeTargets Rogi2 genomic region with HDR and expresses LAMB3-T2A-GFPDepositorInsertLAMB3-T2A-GFP
UseTagsExpressionMutationPromoterAvailable SinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKO (Kan)
Plasmid#110086PurposeSuicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.DepositorTypeEmpty backboneUseBacterial knockout generationTagsExpressionMutationPromoterAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Brp-T2A-QF2w-Hsp70-3XP3-dsRed
Plasmid#141094PurposeTemplate plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.DepositorInsertbruchpilot
UseCRISPRTagsExpressionInsectMutationcoding sequence downstream of CRISPR cut site, sy…PromoterAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;cldn5bP1Egfp
Plasmid#90158Purposecontains cldn5b specific gene promoter driving expression of eGFPDepositorAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1a-MegaGate-HA-Hygro
Plasmid#175268PurposeMegaGate destination with C-terminal HA tag and hygromycin resistanceDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterEF1aAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB508-cTP-mCherry
Plasmid#213862PurposeBinary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused mCherry-UnaG
UseTagsExpressionBacterial and PlantMutationPromoterAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1a-MegaGate-HA-Blast
Plasmid#175270PurposeMegaGate destination with C-terminal HA tag and blasticidin resistanceDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterEF1aAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF6.HA-ARRDC3
Plasmid#38317DepositorAvailable SinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_LMTK3
Plasmid#187773PurposeMAC-tagged gene expression of human LMTK3DepositorAvailable SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x5
Plasmid#58775PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
A1-20B1(P004)
Plasmid#184056PurposeThis plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.DepositorInsertA1-20B1 preamplifier
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2850
Plasmid#87758PurposePcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.DepositorInsertPcol-19-PH-miniSOG(Q103L)
UseTagsPHExpressionWormMutationQ103LPromoterPcol-19Available SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
TOPO GFP
Plasmid#68488PurposeTOPO construct expressing GFP for generation of GMAP-compatible geneDepositorInsertGreen Fluorescent Protein
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Parp14-c011
Plasmid#173098PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
PNCA-GFP-dYY1
Plasmid#67791PurposeUse together with pCMV-intron and VSV-G to package MMLV reporter virusDepositorInsertMMLV-LTR-GFP
UseTagsExpressionMutationDeletion of LTR U3 YY1 binding domainPromoterAvailable SinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBFv-UAS3
Plasmid#138399Purpose10xUAS expression vector with a vermilion marker and a phiC31 attB sequenceDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterUASAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKO (Apr)
Plasmid#110088PurposeSuicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.DepositorTypeEmpty backboneUseBacterial knockout generationTagsExpressionMutationPromoterAvailable SinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
eGFP L202 gRNA
Plasmid#119132PurposegRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.DepositorInserteGFP L202 gRNA
UseCRISPRTagsExpressionMutationNonePromoterU6Available SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb713-HO
Plasmid#141199PurposeExpresses mRhubarb713 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb713
heme oxygenase
UseTags6x-HisExpressionBacterialMutationR134H, W309R, Q310A compared to iRFPPromoterT7Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymYPET-URA3
Plasmid#168056PurposeReplaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymYPET
UseTagsymYPETExpressionYeastMutationPromoterAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
cmv-sfGFP-GB1-scAB-ubc-nls-pcp-12xSunTag(SRv1.2-2P)
Plasmid#185802Purposeone plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.2DepositorInsertcmv-sfGFP-GB1-scAB--ubc-nls-pcp-12xSunTag
UseTagsExpressionMammalianMutationPromoterAvailable SinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
mito-mGarnet
Plasmid#104309Purposecodes for a fusion of fluorescent protein mGarnet and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mitoDepositorInsertmito-mGarnet
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
mKOkappa-2A-mTurquoise2
Plasmid#98837PurposeProduces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRETDepositorInsertmKOkappa-2A-mTurquoise2
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKO (Hyg)
Plasmid#110087PurposeSuicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.DepositorTypeEmpty backboneUseBacterial knockout generationTagsExpressionMutationPromoterAvailable SinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCPP5741
Plasmid#128722PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcV-hopV1
UseTagsExpressionBacterialMutationPromoterAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5910
Plasmid#128717PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcO-hopO1-1
UseTagsExpressionBacterialMutationPromoterAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5058
Plasmid#128710PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcF-hopF2
UseTagsExpressionBacterialMutationPromoterAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-GFP-mCherry-3pGW
Plasmid#53965PurposeBidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherryDepositorInsertsGFP
mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only