We narrowed to 10,731 results for: Kars

1. pT7-V5-SBP-C1-HsGIGYF1_1-671_AI

   Plasmid

   #148962

   Purpose

   Mammalian Expression of HsGIGYF1_1-671

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

   Insert

   HsGIGYF1_1-671 (GIGYF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-lambdaN-HA-DmCaf40-F65AC69Y_X

   Plasmid

   #147993

   Purpose

   Insect Expression of DmCaf40-F65AC69Y

   Depositor

   Elisa Izaurralde

   Article

   Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

   Insert

   DmCaf40-F65AC69Y (Rcd-1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-lambdaN-HA-DmCaf40_25-291_W

   Plasmid

   #147866

   Purpose

   Insect Expression of DmCaf40_25-291

   Depositor

   Elisa Izaurralde

   Article

   Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

   Insert

   DmCaf40_25-291 (Rcd-1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFUW-TetO-ZNF267

   Plasmid

   #178452

   Purpose

   doxycycline-inducible expression of Human ZNF267 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   ZNF267 (ZNF267 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFUW-TetO-PLEK

   Plasmid

   #178439

   Purpose

   doxycycline-inducible expression of Human PLEK in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   PLEK (PLEK Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p406 – TDH3-ER-FlucDM-GFP- Ura

   Plasmid

   #177732

   Purpose

   DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA marker

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   FlucDM
   

   Use

   Tags

   HA, HDEL, KAR2 Signal Sequence, and eGFP

   Expression

   Bacterial and Yeast

   Mutation

   R188Q, R261Q (Destabilized Mutant)

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-Hygro-EF1A-KR-Linker-eGFP

   Plasmid

   #176066

   Purpose

   EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   KR
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLIC-His-GBAi W211A

   Plasmid

   #171756

   Purpose

   For the expression of His-GBAi W211A in bacteria

   Depositor

   Mikel Garcia-Marcos

   Article

   Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

   Insert

   GBAi
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. SIN40C.TRE.dTomato.miR-ctrl.PGK.sfGFP.P2A.Tet3G

   Plasmid

   #169316

   Purpose

   Doxycycline-inducible expression of miR-ctrl with dTomato as reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   miR-ctrl
   

   Use

   Lentiviral

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SIN40C.SFFV.mTagBFP2.miR-ctrl

    Plasmid

    #169312

    Purpose

    Constitutive overexpression of miR-ctrl with mTagBFP2 as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    miR-ctrl
    

    Use

    Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBlastR-5xQUAS-EBFP2-GMRWhite

    Plasmid

    #165921

    Purpose

    Blasticidin resistant 5xQUAS EBFP2 GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable QUAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBlastR-5xQUAS-sfGFP-MiniWhite

    Plasmid

    #165922

    Purpose

    Blasticidin resistant 5xQUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable QUAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBlastR-5xQUAS-EBFP2-MiniWhite

    Plasmid

    #165924

    Purpose

    Blasticidin resistant 5xQUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable QUAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBlastR-5xUAS-EBFP2-MiniWhite

    Plasmid

    #165912

    Purpose

    Blasticidin resistant 5xUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pG418R-MCS-LexA-VP16-MiniWhite

    Plasmid

    #165894

    Purpose

    G418 resistant LexA driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWZL N-Myc-hPOT1_K90E-IRES_hygro

    Plasmid

    #166407

    Purpose

    Express hPOT1 K90E in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 (POT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K90E

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBlastR-5xUAS-mCherry-GMRWhite

    Plasmid

    #165908

    Purpose

    Blasticidin resistant 5xUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBlastR-5xQUAS-mCherry-GMRWhite

    Plasmid

    #165920

    Purpose

    Blasticidin resistant 5xQUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable QUAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBlastR-12xLexAOp-sfGFP-MiniWhite

    Plasmid

    #165916

    Purpose

    Blasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable LexAOp Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBlastR-5xQUAS-mCherry-MiniWhite

    Plasmid

    #165923

    Purpose

    Blasticidin resistant 5xQUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable QUAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET51b-His-TEV-SNAP-tag_dead_mt

    Plasmid

    #167270

    Purpose

    Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    C145A

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET51b-His-TEV-SNAP-tag_fast_dead_mt

    Plasmid

    #167272

    Purpose

    Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R-C145A

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p1A CbbL-

    Plasmid

    #162693

    Purpose

    p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    CbbL K194M (inactive rubisco)

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-EGxxFP-PIGA

    Plasmid

    #153958

    Purpose

    Used for EGxxFP assay with PIGA intron 5 and exon 6 sequences

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    PIGA (a 401-bp fragment from intron 5 and exon 6)
    

    Use

    A plasmid specific for egxxfp assay

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    No mutation

    Promoter

    CAG promoter

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-EGxxFP-CD55

    Plasmid

    #153959

    Purpose

    Used for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequences

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    CD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)
    

    Use

    A plasmid specific for egxxfp assay

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    No mutation

    Promoter

    CAG promoter

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHA852 - mec-4p::FynY531F

    Plasmid

    #139209

    Purpose

    Expresses activated Fyn kinase in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Y531F

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TOG_D1

    Plasmid

    #139112

    Purpose

    express first domain (1-250) of TOG

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    TOG/CKAP5 (CKAP5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    1-250

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. hnRNPA2_LC_RtoK

    Plasmid

    #139120

    Purpose

    express hnRNPA2 LC where all R are K

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all R to K

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hnRNPA2_LC_NtoS

    Plasmid

    #139121

    Purpose

    express hnRNPA2 with all N changed to S

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all N to S

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. hnRNPA2_LC_5E

    Plasmid

    #139122

    Purpose

    express hnRNPA2 with 5 serine changed to glutamate

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    5 serine to glutamate

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. hnRNPA2_LC_12E

    Plasmid

    #139123

    Purpose

    express hnRNPA2 with all serine change to glutamate

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    12 S to E

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. hnRNPF_PLD_YtoS

    Plasmid

    #139124

    Purpose

    express hnRNPF PLD with all Y changed to S

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all Y to S

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. hnRNPF_PLD_StoA

    Plasmid

    #139125

    Purpose

    express hnRNPF PLD with all S changed to A

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all S to A

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJT46_GalL_cCDA1-BE3

    Plasmid

    #145039

    Purpose

    Expresses cCDA1-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    cCDA1-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJT171_GalL_A3AΔ178-BE3

    Plasmid

    #145121

    Purpose

    Expressing base editor A3AΔ178-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ178-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(1-178AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJT170_GalL_A3AΔ182-BE3

    Plasmid

    #145120

    Purpose

    Expressing base editor A3AΔ182-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ182-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(1-182AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJT169_GalL_A3AΔ186-BE3

    Plasmid

    #145119

    Purpose

    Expressing base editor A3AΔ186-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ186-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(1-186AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJT166_GalL_A3A-BE3

    Plasmid

    #145116

    Purpose

    Expressing base editor A3A-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJT139_GalL_nCDA1-VRERBE3

    Plasmid

    #145102

    Purpose

    Expressing base editor nCDA1-VRERBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-VRERBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJT92_GalL_nCDA1Δ188-BE3

    Plasmid

    #145052

    Purpose

    Expresses nCDA1Δ188-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ188-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-188AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJT90_GalL_nCDA1Δ190-BE3

    Plasmid

    #145050

    Purpose

    Expresses nCDA1Δ190-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ190-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-190AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pJT85_GalL_nCDA1Δ193-BE3

    Plasmid

    #145048

    Purpose

    Expresses nCDA1Δ193-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ193-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-193AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pJT84_GalL_nCDA1Δ194-BE3

    Plasmid

    #145047

    Purpose

    Expresses nCDA1Δ194-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ194-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-194AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pJT78_GalL_nCDA1Δ195-BE3

    Plasmid

    #145044

    Purpose

    Expresses nCDA1Δ195-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ195-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-195AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. LMX1B-gRNA-C-del

    Plasmid

    #131516

    Purpose

    gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    LMX1B-gRNA-C-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

46. pET14b-dabAB2 D353A

    Plasmid

    #133016

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    D353A

    Promoter

    t7

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET14b-dabAB2 C351A

    Plasmid

    #133014

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    C351A

    Promoter

    t7

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. hHSF4b K294R

    Plasmid

    #118357

    Purpose

    This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K294R

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pYES260-sc.eEF2 K509A

    Plasmid

    #85120

    Purpose

    yeast expression of mutant EEF2

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K509A

    Promoter

    GAL1

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. Gal4-HSF4b WT

    Plasmid

    #118358

    Purpose

    This plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge