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vYM141_phiC31_nTRE-pEF1s(orig)(CC793)-H2B-citrine

Plasmid

#197684

Purpose

"pEF1s(orig)" reporter plasmid, with CpG793 mutated to CC

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-sgT-REX17_EF1a-Puro-T2A-BFP

Plasmid

#195501

Purpose

Lentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

BFP, Puro, and T2A

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-mGFP-MutCREB(S133A)

Plasmid

#194642

Purpose

Expresses the fusion of monomeric-EGFP-tagged mutant CREB(S133A) in a Cre-dependent manner

Depositor

Jun-Hyeong Cho

Article

Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

Insert

CREB (Creb1 Rat)

Use

AAV

Tags

mEGFP

Expression

Mutation

S133A

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPB EF1a CCR6-eLOV-TEVcs-Gal4

Plasmid

#173119

Purpose

Expresses CCR6 eLOV receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-eLOV-TEVcs-Gal4

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPB EF1a CCR6-hLOV-TEVcs-Gal4

Plasmid

#173120

Purpose

Expresses CCR6 hLOV receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-hLOV-TEVcs-Gal4

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-3xV5-EF1-smFP-flag

Plasmid

#182562

Purpose

For mouse Nav1.6 knockin with 3xV5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and 3xV5 donor DNA
smFP-flag

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

EF1 and U6

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-Ef1A-Myc-POLB(PAMmut)

Plasmid

#176150

Purpose

N-terminus Myc-tagged POLB containing a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

Mutation in the PAM site used by POLB gRNA1

Promoter

EF1A

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154172

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)

Plasmid

#159664

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

Plasmid

#159663

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

Plasmid

#159662

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

Plasmid

#159656

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJM457 EF1alpha EBFP2-P2A-BlastR (TUPV8)

Plasmid

#139237

Purpose

mMoClo TUPV8, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)

Plasmid

#139236

Purpose

mMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJM454 EF1alpha EBFP2-P2A-BlastR (TUPV5)

Plasmid

#139234

Purpose

mMoClo TUPV5, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJM453 EF1alpha EBFP2-P2A-BlastR (TUPV4)

Plasmid

#139233

Purpose

mMoClo TUPV4, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJM452 EF1alpha EBFP2-P2A-BlastR (TUPV3)

Plasmid

#139232

Purpose

mMoClo TUPV3, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJM451 EF1alpha EBFP2-P2A-BlastR (TUPV2)

Plasmid

#139231

Purpose

mMoClo TUPV2, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJM589 EF1α intN-ZF10-NLS-HA in TUPV2

Plasmid

#161565

Purpose

Constitutive expression of intN-ZF10-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

intN-ZF10-NLS-HA

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM516 EF1α intC-ZF1-NLS-HA in TUPV2

Plasmid

#161551

Purpose

Constitutive expression of intC-ZF1-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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