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4,299 results

Showing: 1701 - 1720 of 4299 results

  2. pANAP

    Plasmid
    #48696
    Purpose
    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS
    Depositor
    Peter Schultz
    Article
    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540
    Inserts
    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    Expression
    Mammalian
    Mutation
    mutant E.coli leucyl synthetase specific for Anap…
    Promoter
    CMV and human H1 promoter
    Available Since
    Nov. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    48696-plasmid-map-sequence-id-197837

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  4. pAAV-hSyn-Cre-P2A-dTomato

    Plasmid
    #107738
    Purpose
    Can be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.
    Depositor
    Rylan Larsen
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    Cre, dTomato
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107738-plasmid-map-sequence-id-207220

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  6. MSCV-Cre

    Plasmid
    #24064
    Purpose
    Retroviral coexpression of Cre and EGFP
    Depositor
    Dan Littman
    Insert
    Cre
    Use
    Cre/Lox and Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    MESV 5' LTR
    Available Since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    24064-plasmid-map-sequence-id-322907

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  9. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Article
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available Since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

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  11. pMCB320

    Plasmid
    #89359
    Purpose
    3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites
    Depositor
    Michael Bassik
    Article
    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103
    Insert
    puromycin resistance, mCherry
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    89359-plasmid-map-sequence-id-176457

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  13. pUC gRNA Shuttle

    Plasmid
    #47024
    Purpose
    Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.
    Depositor
    Wayne Parrott
    Article
    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:
    Insert
    gRNA Shuttle
    Use
    CRISPR; Cas9
    Expression
    Plant
    Mutation
    G to T cloning mutation at position 323
    Promoter
    Medicago truncatula U6.6
    Available Since
    Sept. 3, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47024-plasmid-map-sequence-id-67042

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  17. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid
    #103865
    Purpose
    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Insert
    PspCas13b
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    Contains the H133A and H1058A mutations which ina…
    Promoter
    EF1a
    Available Since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    103865-plasmid-map-sequence-id-243540

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  20. APEX2-OMM

    Plasmid
    #79056
    Purpose
    Soybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling protein
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    Soybean APEX2
    Use
    Lentiviral
    Tags
    flag and last 31 amino acids of human MAVS
    Expression
    Bacterial and Mammalian
    Promoter
    CMV immearly promotor
    Available Since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    79056-plasmid-map-sequence-id-238450

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Showing: 1701 - 1720 of 4299 results