4,202 results

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EB3-tdTomato

Plasmid

#50708

Purpose

expresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

Depositor

Erik Dent

Article

Merriam et al J Neurosci. 2013 Oct 16;33(42):16471-82.

Insert

EB3 (MAPRE3 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Blast V5-LUC (w528-1)

Plasmid

#19166

Purpose

3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Lentiviral and Luciferase

Tags

V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-FRB

Plasmid

#25919

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

FRB (MTOR Human)

Expression

Mammalian

Available Since

Sept. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pHR_5x Gal4 UAS

Plasmid

#79119

Purpose

Base vector for cloning Gal4DBDVP64 inducible genes

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCIG3 (pCMV-IRES-GFP version 3)

Plasmid

#78264

Purpose

Lentiviral empty cloning vector containing IRES and GFP

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV, lentiviral LTR

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-3xFLAG-gateway-EGFP

Plasmid

#122845

Purpose

N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway destination

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 236HSC WT (BRCA2)

Plasmid

#16246

Depositor

Mien-Chie Hung

Article

Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

Insert

BRCA2 (BRCA2 Human)

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pC019 - LwCas13a from Leptotrichia wadei

Plasmid

#91909

Purpose

Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a and guide expression cassette

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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HDM-SARS2-Spike-delta21

Plasmid

#155130

Purpose

Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping

Depositor

Jesse Bloom

Article

Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

Insert

SARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized to H. sapiens using IDT codon opt…

Promoter

CMV

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rab5B

Plasmid

#61802

Purpose

Human Rab5B fluorescently tagged with GFP on the N-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

Insert

RAB5B (RAB5B Human)

Tags

GFP

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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Human IFN-gamma promoter/enhancer

Plasmid

#17598

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter with 1st intron enhancer (IFNG Human)

Tags

luciferase

Expression

Mammalian

Mutation

-3.6 kb to +70 of promoter plus 680 bp of IFN-gam…

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV4 p50

Plasmid

#21965

Depositor

Warner Greene

Article

Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

Insert

NF-kB p50 subunit (NFKB1 Human)

Expression

Mammalian

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pSBbi-Neo

Plasmid

#60525

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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AAV CMV-dSaCas9-KRAB-bGHpA

Plasmid

#106219

Purpose

AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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Rtn4a-GFP

Plasmid

#61807

Purpose

Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

Insert

Rtn4a (RTN4 Human)

Tags

AcGFP

Expression

Mammalian

Mutation

Rtn4a Stop Codon removed

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pgRNA-humanized

Plasmid

#44248

Purpose

Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

guide RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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pF155 pcDNA3.1(+)SOD1G93A

Plasmid

#26401

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

G93A

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-APOBEC1-YTH

Plasmid

#131636

Purpose

Expresses APOBEC1 fused to the YTH domain

Depositor

Kate Meyer

Article

Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

Insert

APOBEC1 fused to YTH domain (YTHDF2 Rat, Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pIS0

Plasmid

#12178

Purpose

3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production

Depositor

David Bartel

Article

Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

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N-Cadherin-EGFP

Plasmid

#18870

Depositor

Valeri Vasioukhin

Article

Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

Insert

N-Cadherin (Cdh2 Mouse)

Tags

EGFP, Kanamycin, and N-Cadherin

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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