19,623 results

1. GST-RH▲-MNase

   Plasmid

   #136292

   Purpose

   Expression of catalytic inactive RNase H fused to micrococcal nuclease

   Depositor

   Kavitha Sarma

   Article

   Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

   Insert

   RNase HI-D10R-E48R and MNase
   

   Expression

   Bacterial

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV hUbC-Cas9-T2A-GFP

   Plasmid

   #53190

   Purpose

   3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized Cas9 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. FSW_jGCaMP8f

   Plasmid

   #197034

   Purpose

   Express GCaMP8f from hSyn1 promoter

   Depositor

   Ronald Hart

   Insert

   GCaMP8f
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST-MNase

   Plasmid

   #136291

   Purpose

   Expresses Micrococcal nuclease

   Depositor

   Kavitha Sarma

   Article

   Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

   Insert

   MNase
   

   Expression

   Bacterial

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti.PGK.blast-Renilla_Luciferase

   Plasmid

   #74444

   Purpose

   Control lentiviral vector expressing Renilla luciferase

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Renilla Luciferase
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-6p1-GST-ArcFL

   Plasmid

   #119877

   Purpose

   Bacterial Expression construct for protein production and purification

   Depositor

   Jason Shepherd

   Article

   Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

   Insert

   Activity Regulated Cytoskeletal Protein (Arc Rat)
   

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUW-tetO-hOKMS

   Plasmid

   #51543

   Purpose

   Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

   Depositor

   Tarjei Mikkelsen

   Article

   Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

   Insert

   hOKMS (MYC, KLF4, SOX2, POU5F1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE2 GFP-Rtn4a

   Plasmid

   #86684

   Purpose

   Lentivirus to stably express fluorescent human protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   Rtn4a (RTN4 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-DIO EYFP

   Plasmid

   #27056

   Depositor

   Karl Deisseroth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

   Insert

   EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEG BacMam

    Plasmid

    #160451

    Purpose

    vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. wtBxb1

    Plasmid

    #222337

    Purpose

    Plasmid for expressing wild type Bxb1 in mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized wild type Bxb1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330.puro

    Plasmid

    #110403

    Purpose

    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe K-Ras 12V

    Plasmid

    #12544

    Depositor

    Channing Der

    Article

    Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

    Insert

    K-Ras 12V (KRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    12V constituitively activated

    Available Since

    Sept. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-TRE3G-EGFP

    Plasmid

    #52343

    Purpose

    AAVS1 donor vector for inducible gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-FOXO3a WT

    Plasmid

    #8360

    Depositor

    Michael Greenberg

    Article

    Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. GLUT1-GFP

    Plasmid

    #89440

    Purpose

    GLUT1 construct

    Depositor

    Jeffrey Rathmell

    Article

    Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

    Insert

    GLUT1 (Slc2a1 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

    Plasmid

    #83841

    Purpose

    Fluorescent probe for G1/S transition and M/G1 transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Synthetic, Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-CreERT2

    Plasmid

    #14797

    Purpose

    Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a(+)-HIV RT-6xHis-RBS-prot

    Plasmid

    #159149

    Purpose

    expresses His-tagged HIV reverse transcriptase and untagged HIV protease

    Depositor

    Andrea Pauli

    Article

    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

    Inserts

    Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
    Human immunodeficiency virus 1 (HIV-1) protease
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    HIV-1 group M/subtype B – BH10 strain

    Available Since

    Sept. 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pRRL Luciferase

    Plasmid

    #120798

    Purpose

    For production of lentivirus to express luciferase, primarily for in vivo bioluminescence tracking experiments.

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Luciferase
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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