We narrowed to 7,460 results for: Lif

1. Sema6a.a-Fc-His

   Plasmid

   #72163

   Purpose

   Expresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema6a.a (Sema6a Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV HA hRB delta CDK + S249

   Plasmid

   #58908

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S249 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S249 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. Sema6b(L)-Fc-His

   Plasmid

   #72165

   Purpose

   Expresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema6b (Sema6b Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Sema3f(L,+)-Fc-His

   Plasmid

   #72147

   Purpose

   Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3f (Sema3f Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Sema4c-Fc-His

   Plasmid

   #72156

   Purpose

   Expresses the extracellular region of the Sema4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema4c (Sema4c Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Sema3a(L, del3)-AP-His

   Plasmid

   #72010

   Purpose

   Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3a (Sema3a Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Ncam1_1.0.1-AP-His

   Plasmid

   #71961

   Purpose

   Expresses the extracellular region of the NCAM1, isoform 1.0.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ncam1_1.0.1 (Ncam1 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEntr1a-mcherry-FKBP-TIAM1(DHPH)

   Plasmid

   #176135

   Purpose

   Heterodimerization with FRB (rapamycin), Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   See Depositor Comments Below

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGY416

   Plasmid

   #166660

   Purpose

   For light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeast

   Depositor

   Gaël Yvert

   Article

   Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

   Insert

   LiCre
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Cre: E340A, D341A

   Promoter

   GAL1

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Venus-Venus-FLARE-AKAR

    Plasmid

    #123331

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-Venus-FLARE-AKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Sema3b(L)-AP-His

    Plasmid

    #72013

    Purpose

    Expresses the Sema3B protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3b (Sema3b Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. His10-B1 Integrin

    Plasmid

    #186139

    Purpose

    For expression of recombinant his10-beta1 integrin cytosolic domain in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Tags

    MBP + uncleavable His10

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Sema3a(S)-AP-His

    Plasmid

    #72012

    Purpose

    Expresses the Sema3A protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3a (Sema3a Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEMT-GDNF

    Plasmid

    #119897

    Purpose

    Mouse GDNF In situ hybridization probe

    Depositor

    Wenqin Luo

    Article

    Fleming et al Elife. 2015 Apr 2;4:e06828. doi: 10.7554

    Insert

    glial cell line derived neurotrophic factor (Gdnf Mouse)
    

    Use

    In situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV

    Plasmid

    #123342

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-EKAR-EV
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Ntng2.a-Fc-His

    Plasmid

    #72120

    Purpose

    Expresses the extracellular region of the Netrin G2, isoform a protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntng2.a (Ntng2 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Ntng1.b-Fc-His

    Plasmid

    #72111

    Purpose

    Expresses the extracellular region of the Netrin G1, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntng1.b (Ntng1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGY466

    Plasmid

    #166661

    Purpose

    For light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeast

    Depositor

    Gaël Yvert

    Article

    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

    Insert

    LiCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Cre: E340A, D341A

    Promoter

    MET17

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Sdk1-AP-His

    Plasmid

    #72008

    Purpose

    Expresses the extracellular region of the Sdk1 protein (endogenous signal peptide replaced with CD33 signal peptide), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sdk1 (Sdk1 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ncam1_1.0.1-Fc-His

    Plasmid

    #72087

    Purpose

    Expresses the extracellular region of the NCAM1, isoform 1.0.1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ncam1_1.0.1 Mouse (Ncam1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. C211

    Plasmid

    #174360

    Purpose

    For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (PABPC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I110L, D111E, D117E, A121G, G139A, Y140F, T147S, …

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EGFP-uTEV1-MAVS-mTA

    Plasmid

    #170999

    Purpose

    HiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[R537A]
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[R537A]

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Sdk1-Fc-His

    Plasmid

    #72134

    Purpose

    Expresses the extracellular region of the Sdk1 protein (endogenous signal peptide replaced with CD33 signal peptide), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sdk1 (Sdk1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV HA hRb delta CDK + S230

    Plasmid

    #58907

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S230 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pCMV HA hRB delta CDK + T252

    Plasmid

    #58910

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T252 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

26. Plxna3-AP-His

    Plasmid

    #71998

    Purpose

    Expresses the extracellular region of the PlexinA3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxna3 (Plxna3 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Sema4d-AP-His

    Plasmid

    #72031

    Purpose

    Expresses the extracellular region of the Sema4D protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4d (Sema4d Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28b Me31B-3Myc

    Plasmid

    #113001

    Purpose

    For protein expression and purification of Myc-tagged full-length Drosophila me31B

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length me31B (me31B Fly)
    

    Use

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-22HT-MSI1(7-103)

    Plasmid

    #60357

    Purpose

    Express the RRM1 of mouse MSI1 in bacterial cells for protein purification

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-103) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    contains amino acids 7-103 of Msi1

    Promoter

    T7

    Available since

    Nov. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

30. Nrp2.5-Fc-His

    Plasmid

    #72102

    Purpose

    Expresses the extracellular region of the Neuropilin 2, isoform 5 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Nrp2.5 (Nrp2 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge