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Plasmid#173184PurposeCRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 (EZH2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C
Plasmid#90014PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6CDepositorInsertsCBirA*-Ago2
NBirA*-TNRC6C
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-short Cre-on G/W
Plasmid#86949PurposeGateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. For introducing large constructs into AAVDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXD4 (murine) MSCV
Plasmid#8549DepositorInsertHOXD4 (Hoxd4 Mouse)
UseRetroviralTagsHis and T7ExpressionMammalianMutationcontains T7 and HIS tags from pET; makes protein …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP
Plasmid#90012PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFPDepositorInsertsCBirA*-Cdc25C
NBirA*-GFP
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant PKA-Cα-mEGFP
Plasmid#114143PurposeMammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertmouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCHOP10(dLZ).pCDNA1
Plasmid#21900DepositorInsertCHOP10 (Ddit3 Mouse)
UseTagsExpressionMammalianMutationThe coding region of the murine CHOP-10 cDNA was …PromoterAvailable sinceOct. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
HOXB7 (human) pCMV
Plasmid#8539DepositorInsertHOXB7 (HOXB7 Human)
UseTagsFLAGExpressionMammalianMutationstarts at AA#1 preceeded by FLAG and Ser-Arg-Ile…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIVT/CamKII gamma CA
Plasmid#122142PurposeFor making constitutively active CamKII gamma RNADepositorInsertconstitutively active CamKII gamma (Camk2g Mouse)
UseFor in vitro transcriptionTagsExpressionMutationFirst 291 amino acid residues of CaMKII gammaPromoterT7Available sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDP1.2
Plasmid#171092PurposeExpresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor)DepositorInsertAAVS1-CMV-OsTIR1-mEmerald-Geminin-Puro-AAVS1
UseSynthetic BiologyTagsEmerald, GemininExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9
Plasmid#163644PurposeMakes a lentivirus that expresses mNeonGreen and Cas9.DepositorInsertmNeonGreen-P2A-Cas9
UseLentiviralTagsmNeonGreen-P2AExpressionMutationPromoterEFSAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
HOXB3 (human) HIS-tag pET
Plasmid#8524DepositorInsertHOXB3 (HOXB3 Human)
UseTagsHis and T7ExpressionBacterialMutationContains 5 additional amino acids before ATG from…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e
Plasmid#90011PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3eDepositorInsertsCBirA*-Cdc25C S216A
NBirA*-14-3-3e
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationSer 216 to AlaPromoterCMVAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXD4 (murine) HIS-tag pET
Plasmid#8548DepositorInsertHOXD4 (Hoxd4 Mouse)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by Western and by in vitro transcri…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
P63-sp64 Poly A
Plasmid#59435Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 63
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
P29-sp64 Poly A
Plasmid#59434Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 29
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
HOXB9 (murine) HIS-tag pET
Plasmid#8541DepositorInsertHOXB9 (Hoxb9 Mouse)
UseTagsHis and T7ExpressionBacterialMutationMissing the first 13 amino acids of HOXB9; makes …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
P21-sp64 Poly A
Plasmid#59433Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 21
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-H
Plasmid#223966PurposeAnti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-L
Plasmid#223967PurposeAnti-MT-C34 light chain expression plasmid; to be used with the heavy chain plasmid, pMT-C34-L (Plasmid 223966), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 light chain jointed to constant region of human IgG1 light chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd
Plasmid#216171PurposeExpress the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ATG13 (1-197)
Plasmid#188074PurposeTo make ATG101/ATG13 HORMA complex for expression in mammalian cellsDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationcodon-optimized ATG13 (1-197)PromoterCMVAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_charge
Plasmid#139126Purposeexpress FUS LC with an hnRNPA2 LC-like charge patterningDepositorInsertFUS (FUS Human)
UseTagsExpressionBacterialMutationAdd R, K, D to make FUS LC have similar charge pa…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoBb.1
Plasmid#121898PurposePlasmid for making zebrafish apoBb.1 in situ probeDepositorInsertApoBb.1 (apobb.1 Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoA-IVb.1
Plasmid#121903PurposePlasmid for making zebrafish apoA-IVb.1 in situ probeDepositorInsertApoa4b.1 (apoa4b.1 Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoA-IVb.2
Plasmid#121904PurposePlasmid for making zebrafish apoA-IVb.2 in situ probeDepositorInsertApoa4b.2 (apoa4b.2 Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta 6X mutant
Plasmid#128816PurposeTo make virus expressing Doc2beta 6X mutantDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsIRES-EGFPExpressionMutationD163A, D218A, D220N, D303A, D357A, D359APromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pCS2 LRP6-eGFP
Plasmid#180143Purposeto make movies of LRP6 vesiclesDepositorInsertHuman LRP6 (LRP6 Human)
UseTagsExpressionMammalianMutationPromoterSP6Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB
Plasmid#110820PurposeConventional dCas9 repressor-dCas9-KRABDepositorInsertdCas9-KRAB
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB-MeCP2
Plasmid#110821PurposeImproved dCas9 repressor-dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB-CAGGS-dCas9-KRAB
Plasmid#110822PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB-CAGGS-dCas9
Plasmid#110823PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track Flag HA PGC-1 alpha
Plasmid#14426DepositorInsertPGC-1 alpha (Ppargc1a Mouse)
UseAdenoviralTagsFlag and HAExpressionMammalianMutationLast four amino acids of PGC-1a removed to make t…PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
HOXB7 (human) FLAG pSP65
Plasmid#8537DepositorInsertHOXB7 (HOXB7 Human)
UseIn vitro transcription/translationTagsFLAGExpressionBacterialMutationstarts at AA#1 preceeded by FLAG and Ser-Arg-Ile…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl-2-s2193
Plasmid#166750PurposeExpress mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.DepositorInsertBcl-2 (BCL2 Human)
UseTagsmCerulean3ExpressionMammalianMutationPromoterHuman ferritinAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-mitf
Plasmid#69801PurposeUsed to make mitf knockout porcine cell lineDepositorInsertsmitf-sgRNA
SpCas9
UseTagsExpressionMammalianMutationPromoterChicken Beta-Actin and U6Available sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/GFP-TTP
Plasmid#141190PurposeExpresses GFP-TTP in mammalian cells, can be used to make inducible cell lineDepositorInsertTTP (ZFP36 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTBL680 CHYRON3 integration construct
Plasmid#126448PurposeTo integrate the CHYRON3 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON3 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pegRNA-HEK3_del1-5-NGG
Plasmid#185478PurposepegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.DepositorInsertHEK3_del1-5-NGG pegRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CaMKIV-DCTK75E
Plasmid#126423PurposeA kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)DepositorInsertcalcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
UseTagsFLAGExpressionMammalianMutationchanged lysine 75 to glutamic acidPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only