We narrowed to 18,759 results for: nls
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Plasmid#183399PurposeDual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleusDepositorInsertGI-VP16-IRES-GAL4DBD-NanoLuc-FKF1
ExpressionMammalianPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS2
Plasmid#69228PurposeExpresses R1335K mutant SpCas9 fused to ZFP-TS2 in mammalian cellDepositorInsertTS2 ZFP
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3
Plasmid#69229PurposeExpresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cellDepositorInsertTS3 ZFP
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS4
Plasmid#69230PurposeExpresses R1335K mutant SpCas9 fused to ZFP-TS4 in mammalian cellDepositorInsertTS4 ZFP
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBGreen-FIRE-puro
Plasmid#139195PurposeLuminescent reporter of ERK activityDepositorInsertCBG99
UseLuciferase and RetroviralTagsFra1 PEST domain fragment (FIRE) and nuclear loca…ExpressionMammalianMutationSee depositor comments belowAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIG-V5-Snail2-IRES-nls-GFP
Plasmid#44282DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertV5-Snail2 (SNAI2 Chicken)
TagsV5ExpressionMammalianPromoterCMV-IE-enhancer and chicken beta-actin promoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGZ136 (pRS406-pREB1-3FLAG-MNase-NLS)
Plasmid#72273PurposeFLAG-Mnase expressed under the REB1 promoterDepositorInsert3xFLAG tag and aa 83-231 of Mnase
PromoterREB1Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
Plasmid#222990PurposeBacterial expression of iGeoCas9(C1) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialPromotertacAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3H-BE3
Plasmid#113416PurposeExpresses hA3H-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthA3H-BE3 (APOBEC3H Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV 4xSTAR-mScarletI-NLS-blast
Plasmid#136260PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagsmScarletI-NLSExpressionMammalianAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
A3Bi-ctd-Cas9n-UGI-NLS
Plasmid#109426PurposeExpresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
UseCRISPRExpressionMammalianMutationInsertion of an L1 intron into the coding sequenc…PromoterCMVAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PHY23-phage-ubc-nls-ha-stdMCP-tdTomato
Plasmid#164044PurposeExpression of stdMCP-tdTomato in mammalian cellsDepositorInsertstdMCP-tdTomato
UseLentiviralExpressionMammalianPromoterhuman ubiquitin C (UBC)Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA
Plasmid#47453PurposeepiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression.DepositorInsertTALE (N136,Grm2,C63)-cib1
UseAAV; TaleTagsHAExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE3-FNLS(RA)
Plasmid#112671PurposeCMV expression vector for BE3-FNLS construct (codon optimized)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE3-FNLS
Tags3x FLAGExpressionMammalianMutationNLS sequence at the N-terminus and D10APromoterCMVAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1
Plasmid#184294PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1DepositorInsertsgRNA-Adrb1
UseAAV and CRISPRExpressionMammalianAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adra1b
Plasmid#184293PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adra1bDepositorInsertssgRNA-Adra1b
sgRNA-Adra1b
UseAAV and CRISPRExpressionMammalianAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpa;U6::BsaI-sgRNA-Grpr1
Plasmid#175176PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting GrprDepositorInsertsgRNA-Grpr
UseAAV and CRISPRAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-NLS
Plasmid#103819PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPHR-mCherry-NLS (CRY2 Mustard Weed)
ExpressionMammalianPromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)
Plasmid#100944PurposeSingle chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tagDepositorInsertscFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Human, Synthetic)
Tags3xTy1ExpressionMammalianMutationF636A, E660A, E725A, R295AAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV ArcLightCo (Q239)-T2A-nls-mCherry
Plasmid#85806PurposeGenetically encoded voltage sensor ArcLight- codon optimizedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertArcLightCo-Q239
TagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
8xGliBS-IVS2-mCherry-NLS-polyA-Tol2
Plasmid#84604PurposeTol2-based hedgehog signal reporter plasmid for zebrafish transgenesisDepositorInsertmCherry
UseZebrafish transgenesisPromoter8xGliBS-delta-crystallin minimal promoterAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
hfDECOR-F148A
Plasmid#219546PurposeExpression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthfDECOR-F148A
UseCRISPRExpressionMammalianMutationChanged TadA8e Phenylalanine 148 to AlaninePromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
hfDECOR-V106G
Plasmid#219547PurposeExpression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthfDECOR-V106G
UseCRISPRExpressionMammalianMutationChanged TadA8e Valine 106 to GlycinePromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET-HiFi SpCas9-NLS-6xHis
Plasmid#207376PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHiFi SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR691APromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mRFP-NLS-PCNA (2514)
Plasmid#61850Purposecell cycle markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCNA (PCNA Human)
TagsNLS and mRFPExpressionMammalianPromoterCMVAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1aL-nlsGFP-W
Plasmid#126688PurposeConstitutive, ubiquitous, nuclear-localized GFP expression.DepositorInsertnlsGFP
UseLentiviralTags6xHIS and NLSPromoterEF1aLAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RANLS-LEHD-BNES
Plasmid#60973PurposeExpresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddRFP A and ddRFP B
TagsCaspase9 substrate sequence (LEHD) is after NLS s…ExpressionMammalianPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSNG-P2A-Puro
Plasmid#136900PurposeLentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)DepositorInsertFNLSNG
UseLentiviralTagsFLAG-NLS and NLSMutationL1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337RPromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
416Gal-SV40NLS-FUS-YFP
Plasmid#29595DepositorAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-BPNLS-CE-ABE8e-SpRY--BPNLS-P2A-EGFP (NK289)
Plasmid#208293PurposeCMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpRY A-to-G base editor and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpCMV-T7-BPNLS-CE-ABE8e-SpRY-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA inlaid into nSpRY(D10A/A6…PromoterCMV and T7Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIG-Flag-Sox9-IRES-nls-GFP
Plasmid#44281DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFlag-Sox9 (SOX9 Chicken)
TagsFlagExpressionMammalianPromoterCMV-IE enhancer and Chicken beta-actin promoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p16 nlsGPS2
Plasmid#228093PurposeExpression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)DepositorInsertnlsGPS2
Tags6xHis-T7 XpressTag and cMycExpressionPlantPromoterp16Available sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAdx-CMV-YFP
Plasmid#73348PurposeExpress YFP-NLS under CMV promoterDepositorInsertCMV-YFP
UseAdenoviralPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-BRX-eGFP-NLS
Plasmid#217534PurposeAAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivoDepositorPublicationInsertBMP reporter element and miniXon casette (Smad1 Mouse)
UseAAVExpressionMammalianPromoterBMP Reporter ElementAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
NLS-FUS-Citrine-PixE
Plasmid#111506PurposeFor the nuclear localization of the PixELL systemDepositorAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-IRES-puro-NLS-HyperdLbCas12a-tagBFP-NLS-3xFlag
Plasmid#236385PurposeOverexpression of HyperdLbCas12a in human cellsDepositorInsertHyperdLbCas12a
UseCRISPR and LentiviralMutationD156R, D235R, E292R, D350RAvailable sinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLbCas12a-EGFP-NLS-3xFlag
Plasmid#236364PurposeOverexpression of dLbCas12a in human cellsDepositorInsertdLbCas12a
UseCRISPR and LentiviralMutationWTAvailable sinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdLbCas12a-EGFP-NLS-3xFlag
Plasmid#236367PurposeOverexpression of HyperdLbCas12a in human cellsDepositorInsertHyperdLbCas12a
UseCRISPR and LentiviralMutationD156R, D235R, E292R, D350RAvailable sinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLbCas12aRVRR-EGFP-NLS-3xFlag
Plasmid#236365PurposeOverexpression of dLbCas12aRVRR in human cellsDepositorInsertdLbCas12aRVRR
UseCRISPR and LentiviralMutationG532R, K538V, Y542R, K595RAvailable sinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdFnCas12a-EGFP-NLS-3xFlag
Plasmid#236376PurposeOverexpression of HyperdFnCas12a in human cellsDepositorInsertHyperdFnCas12a
UseCRISPR and LentiviralMutationE184R, T279R, D340R, D409RAvailable sinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only