We narrowed to 8,554 results for: sgRNA

1. pBHM2650

   Plasmid

   #229992

   Purpose

   Carries AID* tag, empty sgRNA, and HYGR marker

   Depositor

   Hiten Madhani

   Insert

   AID* - empty sgRNA - HYGR
   

   Use

   Tags

   2xFLAG

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBHM2652

   Plasmid

   #229994

   Purpose

   Carries AID* tag, empty sgRNA, and amdS marker

   Depositor

   Hiten Madhani

   Insert

   AID* - empty sgRNA - amdS
   

   Use

   Tags

   2xFLAG

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDECKO_GFP_AAVS1_186

   Plasmid

   #159091

   Purpose

   targeting, non-essential locus control

   Depositor

   Rory Johnson

   Insert

   gRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDECKO_mCherry_AAVS1

   Plasmid

   #141212

   Purpose

   pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control

   Depositor

   Rory Johnson

   Insert

   gRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA telo/GFP

   Plasmid

   #137713

   Purpose

   Vector co-expressing sgRNA telo and GFP

   Depositor

   Dominik Niopek

   Insert

   sgRNA telo + GFP
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6/RSV

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMZ374

   Plasmid

   #59896

   Purpose

   Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   adh1 and rrk1

   Available since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. phU6 ST

   Plasmid

   #188712

   Purpose

   sgRNA plasmid encoding hU6 promoter driving an anti-safe targeting sgRNA

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Insert

   Safe Targeting sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmU6 ST

   Plasmid

   #188713

   Purpose

   sgRNA plasmid encoding mU6 promoter driving an anti-safe targeting sgRNA

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Insert

   Safe Targeting sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p7SK ST

   Plasmid

   #188714

   Purpose

   sgRNA plasmid encoding 7SK promoter driving an anti-safe targeting sgRNA

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Insert

   Safe Targeting sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. phH1 ST

    Plasmid

    #188715

    Purpose

    sgRNA plasmid encoding hH1 promoter driving an anti-safe targeting sgRNA

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    Safe Targeting sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid

    #208647

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid

    #208979

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. phU6 NFATC2

    Plasmid

    #188708

    Purpose

    sgRNA plasmid encoding hU6 promoter driving an anti-NFATC2 sgRNA

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    NFATC2 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmU6 NFATC2

    Plasmid

    #188709

    Purpose

    sgRNA plasmid encoding mU6 promoter driving an anti-NFATC2 sgRNA

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    NFATC2 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p7SK NFATC2

    Plasmid

    #188710

    Purpose

    sgRNA plasmid encoding 7SK promoter driving an anti-NFATC2 sgRNA

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    NFATC2 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. phH1 NFATC2

    Plasmid

    #188711

    Purpose

    sgRNA plasmid encoding hH1 promoter driving an anti-NFATC2 sgRNA

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    NFATC2 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCJH026_proD_Cas12c-multiplexing_CAM

    Plasmid

    #183073

    Purpose

    Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBHM2651

    Plasmid

    #229993

    Purpose

    Carries AID* tag, empty sgRNA, and NEOR marker

    Depositor

    Hiten Madhani

    Insert

    AID* - empty sgRNA - NEOR
    

    Use

    Tags

    2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR223-CMV-Cre-U6-sgC0111-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

    Plasmid

    #209062

    Purpose

    Entry vector that encodes sgRNAs against mouse Rb1, Trp53, Rbl2, and a non-targeting sgRNA (C0111) and CMV Cre recombinase.

    Depositor

    Matthew Oser

    Article

    Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

    Insert

    non-targeting sgRNA (C0111)
    

    Use

    Gateway vector to be used for lr reaction

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiGuide-Hygro

    Plasmid

    #139462

    Purpose

    Lentiviral vector with gRNA scaffold and hygromycin selectable marker

    Depositor

    Caroline Goujon

    Insert

    no sgRNA inserted; resistance gene: hygroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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