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We narrowed to 5,413 results for: PEP

Showing: 4301 - 4350 of 5413 results

  8. pET28-AviTag-DogTag-MBP

    Plasmid
    #171773
    Purpose
    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogTag-MBP
    Use
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171773-plasmid-map-sequence-id-338517

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  11. pExpreS2-1-C3-PA-10H-SIII

    Plasmid
    #175443
    Purpose
    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Use
    Tags
    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…
    Expression
    Insect
    Mutation
    Promoter
    Fused Actin-HSP70
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    175443-plasmid-map-sequence-id-360135

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  14. UTS2R-DuET

    Plasmid
    #213386
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    UTS2R (UTS2R Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213386-plasmid-map-sequence-id-430893

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  15. pQCXIP-mCherry-CDMPR

    Plasmid
    #182643
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by mCherry and murine CDMPR (without its signal peptide)
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    CDMPR
    Use
    Retroviral
    Tags
    N-terminal ER targeting sequence of hemagglutinin…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182643-plasmid-map-sequence-id-358629

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  18. OPN3-DuET

    Plasmid
    #213357
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPN3 (OPN3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213357-plasmid-map-sequence-id-430879

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  19. pQCXIP-EGFP-CDMPR

    Plasmid
    #182642
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by EGFP and murine CDMPR (without its signal peptide)
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    CDMPR
    Use
    Retroviral
    Tags
    EGFP and N-terminal ER targeting sequence of hema…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182642-plasmid-map-sequence-id-358624

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  21. SSTR3-DuET

    Plasmid
    #213378
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR3 (SSTR3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213378-plasmid-map-sequence-id-430734

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  22. pGEM-NLS-Cre-2A-mCherry-FKF

    Plasmid
    #119868
    Purpose
    Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119868-plasmid-map-sequence-id-240979

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  24. pYPQ173

    Plasmid
    #99907
    Purpose
    Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence
    Depositor
    Yiping Qi
    Article
    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(
    Insert
    pco-dCas9-VP64-T2A-MS2-VP64
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    99907-plasmid-map-sequence-id-210432

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  25. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid
    #75154
    Purpose
    Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    H2B-mCerulean3-P2A-mKOFP2-CAAX
    Use
    Middle entry vector for multisite gateway three f…
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75154-plasmid-map-sequence-id-142108

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  26. pCEP4-DU422gp140-TM

    Plasmid
    #123251
    Purpose
    Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tether
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (DU422) Env
    Use
    Tags
    CD5 leader peptide and Linker (with 6his tag) and…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123251-plasmid-map-sequence-id-235207

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  27. P2RY11-DuET

    Plasmid
    #213364
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    P2RY11 (P2RY11 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213364-plasmid-map-sequence-id-430884

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  33. NTSR1-DuET

    Plasmid
    #213356
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    NTSR1 (NTSR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213356-plasmid-map-sequence-id-430917

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  34. pTet-On-TAP

    Plasmid
    #59017
    Purpose
    Construct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.
    Depositor
    David Sibley
    Article
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Type
    Empty backbone
    Use
    Toxoplasma expression
    Tags
    Calmodulin Binding Peptide fused with a TEV Cleav…
    Expression
    Mutation
    Promoter
    RPS13 with Tet Operators Inserted
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59017-plasmid-map-sequence-id-423220

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  35. pYSD313

    Plasmid
    #212931
    Purpose
    Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    Sed1 (SED1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212931-plasmid-map-sequence-id-421768

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  36. pET28a-RBP-LOV2

    Plasmid
    #185792
    Purpose
    Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker
    Depositor
    Lizhong He
    Article
    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864
    Insert
    RBP-LOV2
    Use
    Tags
    10xHis, TEV protease cleavage site after His Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185792-plasmid-map-sequence-id-367546

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  37. pDONR P2R-P3-SV40 polyA signal

    Plasmid
    #48627
    Purpose
    Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.
    Depositor
    Tomas Santalucia, Anna Planas
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    SV40 early polyadenylation signal cassette
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48627-plasmid-map-sequence-id-70632

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  38. OPRD1-DuET

    Plasmid
    #213358
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRD1 (OPRD1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213358-plasmid-map-sequence-id-430880

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  39. SSTR2-DuET

    Plasmid
    #213377
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR2 (SSTR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213377-plasmid-map-sequence-id-430733

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  44. MTNR1A-DuET

    Plasmid
    #213348
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MTNR1A (MTNR1A Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213348-plasmid-map-sequence-id-430874

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  48. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Available since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    128179-plasmid-map-sequence-id-249095

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  49. Nrxn3α-AS4-

    Plasmid
    #184332
    Purpose
    Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.
    Depositor
    Peter Scheiffele
    Article
    Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife
    Insert
    Nrxn3-alpha (Nrxn3 Mouse)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184332-plasmid-map-sequence-id-361409

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Showing: 4301 - 4350 of 5413 results