163,606 results

1. pThermoCas9_ctrl

   Plasmid

   #100981

   Purpose

   Expresses ThermoCas9 and its sgRNA module

   Depositor

   John van der Oost

   Article

   Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

   Inserts

   Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
   ThermoCas9 single guide RNA expressing module
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   B. coagulans DSM 1 pta promoter and B. smithii xy…

   Available Since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFCiR2

   Plasmid

   #167512

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mRFP, Kanamycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CinRAM-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCin

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL.PPT.SFFV.HA.RUNX1C.IRES.eGFP

   Plasmid

   #181980

   Purpose

   Human RUNX1C gene overexpression

   Depositor

   Jan-Henning Klusmann

   Insert

   RUNX1C (human) (RUNX1 Human)
   

   Use

   Lentiviral

   Tags

   HA (Human influenza hemagglutinin)

   Expression

   Mutation

   Promoter

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

   Plasmid

   #159908

   Purpose

   Mutagenesis of Htr2a

   Depositor

   Larry Zweifel

   Insert

   Htr2a (Htr2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. hCMV-IE1:FLuc

   Plasmid

   #118063

   Purpose

   Transcriptional unit for constitutive expression of FLuc in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:FLuc:bGHF
   

   Use

   Luciferase and Synthetic Biology; Assembly of gb2…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-syn-iGluSnFR4s-PDGFR-WPRE

   Plasmid

   #234435

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIneoMyc rat SAPAP1

   Plasmid

   #40215

   Depositor

   Yutaka Hata, Yoshimi Takai

   Article

   Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

   Insert

   SAPAP1 (Dlgap1 Rat)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pMX-Flag-GFP_HOMER2

   Plasmid

   #227953

   Purpose

   Co-localisation experiment

   Depositor

   Alessandro Ori

   Insert

   HOMER2 (HOMER2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a SnoopTag-MBP

   Plasmid

   #72323

   Purpose

   Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a SnoopTag-MBP
   

   Use

   Tags

   N-terminal His6 tag. MBP is fused at the C-termin…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-VASP-5

    Plasmid

    #55151

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    VASP
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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