159,530 results

1. GALT-SEP-mRuby3

   Plasmid

   #200938

   Purpose

   trans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Inserts

   GalT (B4GALT1 Human)
   SEP-mRuby3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti puro HA-Ubiquitin

   Plasmid

   #74218

   Purpose

   Lentiviral expression of HA tagged Ubiquitin

   Depositor

   Melina Fan

   Insert

   ubiquitin (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Single ubiquitin coding sequence (full length UBB…

   Promoter

   CMV

   Available since

   March 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. TFORF3008

   Plasmid

   #144484

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CDX2 (CDX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-puro-N-3HA-SREBP1-C-Flag

   Plasmid

   #134286

   Purpose

   Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SREBP1 (Srebf1 Mouse)
   

   Use

   Lentiviral

   Tags

   3x HA and Flag

   Expression

   Mammalian

   Mutation

   mouse SREBP1 isoform a precursor

   Promoter

   EF1a

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. MGAT-SEP-mRuby3

   Plasmid

   #200937

   Purpose

   cis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Inserts

   MGAT2 (MGAT2 Human)
   SEP-mRuby3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-Vimentin-N-18

   Plasmid

   #55158

   Purpose

   Localization: Intermediate Filaments, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Vimentin (VIM Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

   Plasmid

   #207860

   Purpose

   AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-tdTomato

   Plasmid

   #159275

   Purpose

   Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11

   Insert

   tdTomato
   

   Use

   AAV; S1 knockin donor vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFLAG_WT MDA5

   Plasmid

   #216798

   Purpose

   Expresses wild type (wt) melanoma differentiation-associated protein 5

   Depositor

   Sun Hur

   Insert

   melanoma differentiation-associated protein 5 (IFIH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMPE-ePPEplus

    Plasmid

    #205242

    Purpose

    For multiplex plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Detailed in manuscript

    Promoter

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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