We narrowed to 9,242 results for: ef1

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pJAM1.21_PB-UGCG-PGK-Blast

Plasmid

#205566

Purpose

Express human UGCG

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

UGCG (UGCG Human)

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-Mma PylRS

Plasmid

#140009

Purpose

Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-XMAS-1xStop

Plasmid

#164411

Purpose

Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

mCherry

Use

CRISPR and Synthetic Biology

Tags

T2A-GFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pJAM1.18_PB-SDC4-PGK-Blast

Plasmid

#205563

Purpose

Express human SDC4

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

SDC4 (SDC4 Human)

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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CRISPR-SP-Cas9 reporter

Plasmid

#62733

Purpose

Mammalian CRISPR-SP-Cas9 reporter

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

CRISPR-SP-Cas9 target seq + tdTomato (out of frame)

Use

CRISPR and Lentiviral

Tags

CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

Expression

Mammalian

Promoter

EF1-Alpha

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-EGFP-GPI

Plasmid

#65298

Purpose

synchronize trafficking of EGFP-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Lentiviral

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-mCherry-GPI

Plasmid

#65299

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Lentiviral

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pWPT-/GCCACC-mEGFP-IRES-mCherry

Plasmid

#49235

Purpose

Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Article

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Lentiviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Promoter

EF1-short

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pLV/Guide-mCherry

Plasmid

#170543

Purpose

Expresses mCherry and contains a cassette for U6 driven sgRNA transcription

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

mCherry

Use

CRISPR and Lentiviral

Promoter

Ef1-alpha

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJAM1.17_PB-STEAP3-PGK-Blast

Plasmid

#205562

Purpose

Express human STEAP3

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

STEAP3 (STEAP3 Human)

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pWPI/hPLK2WT/Neo

Plasmid

#35385

Depositor

Anurag Tandon

Article

Visanji et al J Biol Chem. 2011 Oct 14;286(41):35863-7

Insert

hPLK2 (PLK2 Human)

Use

Lentiviral

Tags

EMCV IRES-Neomycin cassette and V5 epitope and Po…

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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HSC1-GiP

Plasmid

#58254

Purpose

This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Retroviral

Expression

Mammalian

Promoter

Human EF1-alpha

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCEP4_WT_OCT4

Plasmid

#40629

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Expression

Mammalian

Promoter

EF1-alpha

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pScalps eSpCas9_Zsgreen gRNA scaffold

Plasmid

#188449

Purpose

Expression of eSpCas9 tagged with Zsgreen

Depositor

Ajit Chande

Article

Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF1-alpha

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEF-ABEmax

Plasmid

#164415

Purpose

EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

ABEmax

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFH2.101_PB-EPN11-MCP-T2A-eGFP-PGK-Blast

Plasmid

#205546

Purpose

Express EPN11-MCP-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-EGFP-Ecadherin

Plasmid

#65290

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Lentiviral

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast

Plasmid

#205549

Purpose

Express EPN24-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPRai

Plasmid

#189938

Purpose

Expresses CRISPRai cassette under EF1a promoter

Depositor

Yu-Chen Hu

Article

Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

Insert

CRISPRai cassette

Expression

Mammalian

Promoter

CMV enhancer-rat EF1 alpha

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-mCherry-Ecadherin

Plasmid

#65291

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Lentiviral

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pWPI/hPLK2K111M/Neo

Plasmid

#35386

Depositor

Anurag Tandon

Article

Visanji et al J Biol Chem. 2011 Oct 14;286(41):35863-7

Insert

hPLK2 K111M (PLK2 Human)

Use

Lentiviral

Tags

EMCV IRES-Neomycin cassette and V5 epitope and Po…

Expression

Mammalian

Mutation

K111M

Promoter

EF1-alpha

Available Since

April 25, 2012

Availability

Academic Institutions and Nonprofits only

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SEP-Nanoluc-cyOFP1

Plasmid

#141082

Purpose

prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEP-Nanoluc-cyOFP1

Use

Lentiviral

Mutation

Deletion of amino acids DISGG from positions 673 …

Promoter

EF1α

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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SGL40C-H1.EFS.RFP657

Plasmid

#69148

Purpose

Advanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

sgRNA
EFS
tagRFP657

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4_OCT4_T234A_S235A

Plasmid

#40630

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1_S234A_T235A (POU5F1 Human)

Expression

Mammalian

Mutation

S234A_T235A

Promoter

EF1-alpha

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pEF-XMAS-2xStop

Plasmid

#164412

Purpose

Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

mCherry

Use

CRISPR and Synthetic Biology

Tags

T2A-GFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4_OCT4_T234E_S235E

Plasmid

#40631

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Expression

Mammalian

Mutation

T234E_S235E

Promoter

EF1-alpha

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pWPT-/CGA-mEGFP-IRES-mCherry

Plasmid

#49233

Purpose

Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Article

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Lentiviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Promoter

EF1-short

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast

Plasmid

#205542

Purpose

Express EPN1-MCP-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH2.96_PB-EPN1-T2A-eGFP-PGK-Blast

Plasmid

#205541

Purpose

Express EPN1-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH2.100_PB-EPN11-T2A-eGFP-PGK-Blast

Plasmid

#205545

Purpose

Express EPN11-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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