We narrowed to 18,831 results for: Tec

1. pCfB4786

   Plasmid

   #106142

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCY 3090-07

   Plasmid

   #36232

   Depositor

   Anne Robinson

   Article

   Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

   Insert

   Streptoalloteichus hindustanus bleomycin (Sh ble)
   

   Use

   Yeast cassette for pcr and homologous recombinati…

   Tags

   yEpolylinker-mCherry-Zeocin

   Expression

   Mutation

   Promoter

   Available Since

   May 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TALEN1259

   Plasmid

   #32284

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   TAL array targeting gria3a (gria3a Zebrafish)
   

   Use

   T7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFREE2-xCas9-sgRNA

   Plasmid

   #179579

   Purpose

   Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   xCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCellFree_G03 SP2

   Plasmid

   #67109

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   SP2 (SP2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pGEX-4T-1-p19-T7-EGFP100

   Plasmid

   #46309

   Depositor

   Judy Lieberman

   Article

   Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

   Inserts

   p19
   EGFP100 DNA-1
   linker
   EGFP100 DNA-2
   

   Use

   Pro-sirna

   Tags

   6XHis and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pASKt15C+SrtA

   Plasmid

   #64983

   Purpose

   Expresses the wild type of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

   Insert

   sortase A
   

   Use

   Tags

   His6 tag and S tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Promoter

   tetracycline promoter/operator

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TALEN1258

   Plasmid

   #32281

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   TAL array targeting gria3a (gria3a Zebrafish)
   

   Use

   T7

   Tags

   3X Flag and FOKI WT

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDM_noro_S2_lacZA

   Plasmid

   #118816

   Purpose

   T7 RNAP-driven expression of norovirus sense orientation toehold switches (S2) with a lacZ alpha subunit reporter.

   Depositor

   Alexander Green

   Article

   Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

   Insert

   Norovirus toehold switch S2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-PRLSS-SH2-KDEL

    Plasmid

    #209716

    Purpose

    Mammalian expression of SH2 localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-SH2-KDEL
    

    Use

    Tags

    PRLSS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BCJJ344

    Plasmid

    #117503

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GWF135

    Plasmid

    #133614

    Purpose

    ANR-ANR-BFP-CAAX

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    ANR-ANR-BFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Cx55.5:rasGFP

    Plasmid

    #105939

    Purpose

    in vitro transcription

    Depositor

    Caren Norden

    Article

    Weber et al Cell Rep. 2014 Apr 24;7(2):386-97. doi:

    Insert

    Ras
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pATD

    Plasmid

    #141012

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    HSV-TK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. tdEos-Talin-18

    Plasmid

    #57672

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Talin
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pDB1751

    Plasmid

    #174013

    Purpose

    pDUAL plasmid expressing GFP from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    GFP
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1 and 41nmt1 promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTMS1

    Plasmid

    #87799

    Purpose

    For constructing MBP-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.

    Depositor

    Yu-Zhu Zhang

    Article

    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

    Type

    Empty backbone

    Use

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 6xHis-MBP-huLb5Cas12a

    Plasmid

    #174684

    Purpose

    Expresses 6xHis-MBP tagged Lb5Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huLb5Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSGV Rh Acceptor

    Plasmid

    #64271

    Purpose

    Retroviral golden gate cloning vector for expression of functional Rhesus Macaque T-Cell Receptor genes

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCellFree_G03 USF2

    Plasmid

    #67069

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    USF2 (USF2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

21. YSBE 596 : pNAT_mCherry

    Plasmid

    #92088

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, NAt selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    mCherry
    

    Use

    tagging proteins in cryptococcus neoforman

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFA6a-zeoCV

    Plasmid

    #73890

    Purpose

    fission yeast drug selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    zeoCV
    

    Use

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p1.2-Zeo

    Plasmid

    #162740

    Purpose

    Protein expression in CHO cells

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CHO Elongation factor 1A

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLpC-L-IFP-F[1] (mammalian)

    Plasmid

    #52901

    Purpose

    Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[1]
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Unknown

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pLpC-L-IFP-F[2] (mammalian)

    Plasmid

    #52902

    Purpose

    Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[2]
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Unknown

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pCellFree_G03 VPS29

    Plasmid

    #67052

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    VPS29 (VPS29 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pCfB2374

    Plasmid

    #67534

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRS416 Gal-RNQ1-YFP

    Plasmid

    #18685

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Rnq1 (RNQ1 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

29. 6xHis-MBP-huHkCas12a

    Plasmid

    #174683

    Purpose

    Expresses 6xHis-MBP tagged HkCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huHkCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. tdEos-Paxillin-22

    Plasmid

    #57653

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Paxillin
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

31. pJ291_WT_med

    Plasmid

    #128856

    Purpose

    Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDM_noro_S6_lacZA

    Plasmid

    #118817

    Purpose

    T7 RNAP-driven expression of norovirus sense orientation toehold switches (S6) with a lacZ alpha subunit reporter.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    Norovirus toehold switch S6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pVH234-Tier1-PmPGK1-tTA

    Plasmid

    #169574

    Purpose

    Tier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled transactivator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCellFree_G03 NFX1

    Plasmid

    #67131

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    NFX1 (NFX1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

35. GGE020

    Plasmid

    #120777

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position1-3. When using the GG platform for expressing only one gene (T1-3_Lip2)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    T1-3_Lip2
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRSET FLIPE-10u

    Plasmid

    #13538

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207R
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    A207R in binding protein

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBT100.110

    Plasmid

    #122602

    Purpose

    clone CDA1 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    CDA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCfB4796

    Plasmid

    #106131

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMS22H

    Plasmid

    #133904

    Purpose

    Expression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTK101

    Plasmid

    #59561

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) mKate-G8-L7Ae-PEST 4xFF4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge