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Showing: 4351 - 4400 of 9181 results

  5. N229A Lo-MetQ

    Plasmid
    #118268
    Purpose
    N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Insert
    MetQ (N229A) (metQ E. coli)
    Use
    Tags
    10x His Tag and Enterokinase Cleavage Site
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 229 to Alanine (Ala)
    Promoter
    T7 Promoter
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    118268-plasmid-map-sequence-id-228534

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  11. BCD2_BC

    Plasmid
    #66024
    Purpose
    MoClo Basic Part: RBS - BiCistronic Design, high strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD2:C]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66024-plasmid-map-sequence-id-114158

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  15. C0062 (luxR)_CD

    Plasmid
    #66028
    Purpose
    MoClo Basic Part: CDS - Controller protein, luxR repressor/activator (in concert with HSL, represses pLuxR(pR) R0063. Also up-regulates pLuxR(pL) R0062) [C:C0062:D]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Transcription factor
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66028-plasmid-map-sequence-id-114215

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  19. R0040 (pTet)_AB

    Plasmid
    #66008
    Purpose
    MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [A:R0040:B]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Controllable promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66008-plasmid-map-sequence-id-114120

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  20. R0040 (pTet)_EB

    Plasmid
    #66009
    Purpose
    MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [E:R0040:B]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Controllable promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66009-plasmid-map-sequence-id-114123

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  21. R0040 (pTet)_FB

    Plasmid
    #66010
    Purpose
    MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [F:R0040:B]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Controllable promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66010-plasmid-map-sequence-id-114125

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  22. R0040 (pTet)_GB

    Plasmid
    #66011
    Purpose
    MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [G:R0040:B]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Controllable promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66011-plasmid-map-sequence-id-114127

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  23. pZS1[RbsR-T]

    Plasmid
    #60751
    Purpose
    Contains PIq driving expression RbsR-T, the Ribose inducible chimera with the TAN DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Insert
    RbsR-T
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Chimeric LacI/GalR repressor with the TAN DBD and…
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    60751-plasmid-map-sequence-id-97732

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  25. pGM190oriT

    Plasmid
    #115679
    Purpose
    E. coli-Streptomyces shuttle vector for gene expression
    Depositor
    Günther Muth
    Insert
    oriT, aphII, tsr
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tipA
    Available since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    115679-plasmid-map-sequence-id-410983

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  26. pOP3MT

    Plasmid
    #112601
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and MBP fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Avi - 8His-MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112601-plasmid-map-sequence-id-220215

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  27. pOP2HA

    Plasmid
    #112598
    Purpose
    Used for expression of protein in E. coli as non-cleavable C-terminal 8His-Avi tag fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-Avi tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112598-plasmid-map-sequence-id-220206

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  28. pOP3GT

    Plasmid
    #112607
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and GST fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Avi - 8His-GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112607-plasmid-map-sequence-id-220236

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  29. pOP3BT

    Plasmid
    #112603
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and GB1 fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Avi - 8His-GB1
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112603-plasmid-map-sequence-id-220218

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  30. pOP3MP

    Plasmid
    #112600
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and MBP fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Avi - 8His-MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112600-plasmid-map-sequence-id-220212

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  31. pOP3TP

    Plasmid
    #112604
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and thioredoxin fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Avi - 8His-thioredoxin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112604-plasmid-map-sequence-id-220219

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  32. pOP3TT

    Plasmid
    #112605
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and thioredoxin fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Avi - 8His-thioredoxin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112605-plasmid-map-sequence-id-220234

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  33. pOP3BP

    Plasmid
    #112602
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and GB1 fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Avi - 8His-GB1
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112602-plasmid-map-sequence-id-220216

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  34. pOP3GP

    Plasmid
    #112606
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and GST fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Avi - 8His-GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112606-plasmid-map-sequence-id-220235

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  36. pET32a-DEST

    Plasmid
    #84652
    Purpose
    E. coli expression vector derived from pET32 with Gateway cassette.
    Depositor
    Neil Olszewski
    Type
    Empty backbone
    Use
    Tags
    His, S tag, and Trx Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    84652-plasmid-map-sequence-id-162124

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  37. pENTR L1-13XLexAop2-R5

    Plasmid
    #41433
    Depositor
    Steve Stowers
    Insert
    13XLexAop2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41433-plasmid-map-sequence-id-55242

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  38. pENTR L1-13XLexAop2-L4

    Plasmid
    #41434
    Depositor
    Steve Stowers
    Insert
    13XLexAop2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41434-plasmid-map-sequence-id-55244

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  39. pAIP4_1EDG

    Plasmid
    #234144
    Purpose
    Heterologous protein expression of GUNA_RUMCH in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EDG
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234144-plasmid-map-sequence-id-464110

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  49. GFP-itis pBE-t

    Plasmid
    #195342
    Purpose
    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V
    Depositor
    Alexis Komor
    Article
    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris
    Inserts
    ecTadA(8e)-SpCas9-NG
    gRNA ctctacgcgggtcttgtagt
    Use
    CRISPR
    Tags
    Expression
    Mutation
    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…
    Promoter
    Lac and ProC
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195342-plasmid-map-sequence-id-383223

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  50. GFP-itis pBE-nt

    Plasmid
    #195343
    Purpose
    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA
    Depositor
    Alexis Komor
    Article
    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris
    Inserts
    ecTadA(8e)-SpCas9-NG
    gRNA gtgcacgacgccgtatgcga
    Use
    CRISPR
    Tags
    Expression
    Mutation
    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…
    Promoter
    Lac and ProC
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195343-plasmid-map-sequence-id-383225

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Showing: 4351 - 4400 of 9181 results