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pDL026_HA-rb_IgG_LC_15F11

Plasmid

#193623

Purpose

HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

Depositor

Jennifer DeLuca

Publication

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-HA_15F11 rabbit light chain

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-mouse Ezh2 delta SETdomain-IRES-GFP

Plasmid

#91885

Purpose

Retroviral expression of mouse Ezh2 with a SET domain deletion

Depositor

Martine Roussel

Publication

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

SET domain deleted

Promoter

MMLV gag

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86021

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Publication

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain

Promoter

CMV IE

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRN3P_AID-Dam-only

Plasmid

#136065

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Publication

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

AID-Dam only

Use

In vitro transcription

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp30-C1

Plasmid

#134547

Purpose

Expresses YFP-Rpp30 fusion protein

Depositor

Susan Janicki

Publication

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rpp30 (RPP30 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn-HpaBC

Plasmid

#100949

Purpose

Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

Depositor

Mattheos Koffas

Publication

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Inserts

RgTALsyn
HpaB
HpaC

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pmcsg7-mCRT(WT)

Plasmid

#83505

Purpose

Bacterial expression of Calreticulin

Depositor

Malini Raghavan

Publication

Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

Insert

CALR (Calr Mouse)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pOPINK-TRABID (NZF1, aa 1-33)

Plasmid

#65437

Purpose

Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli

Depositor

David Komander

Publication

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

TRABID (ZRANB1 Human)

Tags

His6-GST

Expression

Bacterial

Mutation

only codes for amino acids 1-33 of TRABID, which …

Promoter

T7

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) neo with Ywhag CDS

Plasmid

#61799

Purpose

pcDNA3.1(+) with full length mouse Ywhag CDS

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Ywhag CDS (Ywhag Mouse)

Expression

Mammalian

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-6P1-Ataxin3Q22-C14A

Plasmid

#22113

Depositor

Henry Paulson

Publication

Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

Insert

Ataxin 3 (ATXN3 Human)

Tags

GST

Expression

Bacterial

Mutation

C14A

Available since

Sept. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pOPINK-OTUD6A (OTU, aa 128-288)

Plasmid

#61417

Purpose

Expresses human OTUD6A (OTU domain) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6A (OTUD6A Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-127.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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p15aC-3D1D-5

Plasmid

#121050

Purpose

Bacterial expression of PIS, PI4K and PI3P5K mutant E545K

Depositor

Sanford Simon

Publication

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-phosphate 5-kinase
phosphatidylinositol 3-kinase catalytic subunit p110α of class IA E545K

Tags

myc and none

Expression

Bacterial

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSE360-Prp22

Plasmid

#111284

Purpose

Prp22 WT gene in pSE360 backbone

Depositor

Christine Guthrie

Publication

Noble et al Genetics. 1996 May;143(1):67-80.

Insert

PRP22

Expression

Yeast

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Sestrin2-E451A

Plasmid

#73674

Purpose

overexpression

Depositor

David Sabatini

Publication

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

HA

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-HA-Sestrin2-L261A

Plasmid

#73672

Purpose

overexpression

Depositor

David Sabatini

Publication

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

HA

Expression

Mammalian

Mutation

L261A changed Leucine 261 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pHIS3p:Venus-Tub1+3'UTR::TRP1

Plasmid

#50650

Depositor

Wei-Lih Lee

Publication

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Venus-Tub1

Use

Yeast expression

Tags

Venus

Promoter

HIS3p

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pHIS3p:CloverGFP-Tub1+3'UTR::TRP1

Plasmid

#50648

Depositor

Wei-Lih Lee

Publication

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CloverGFP-Tub1

Use

Yeast expression

Tags

CloverGFP

Promoter

HIS3p

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pHIS3p:mEos2-Tub1+3'UTR::LEU2

Plasmid

#50646

Depositor

Wei-Lih Lee

Publication

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mEos2-Tub1

Use

Yeast expression

Tags

mEos2

Promoter

HIS3p

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pHIS3p:yomWasabi-Tub1+3'UTR::LEU2

Plasmid

#50643

Depositor

Wei-Lih Lee

Publication

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

yomWasabi-Tub1

Use

Yeast expression

Tags

yomWasabi

Promoter

HIS3p

Available since

Dec. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pRS303:3mCherry

Plasmid

#37549

Depositor

Wei-Lih Lee

Publication

Markus et al Curr Biol. 2009 Feb 10;19(3):196-205. Ep

Insert

3mCherry

Expression

Bacterial

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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