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Plasmid#155021PurposeN-terminal 3xFLAG-tagged CUL3 in a pCMV7.1 vectorDepositorInsertCullin 3 (CUL3 Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIV303
Plasmid#204978PurposeAMA1 plasmid with Aspergillus optimized Mad7 and amdS selection markerDepositorInsertsMad7
amdS
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG-CSK-WT
Plasmid#74502PurposeOverexpression of CSK in mammalian cellsDepositorInsertCSK (CSK Human)
UseTagsFLAGExpressionMammalianMutationK222RPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-2xAM
Plasmid#92220PurposeLentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sitesDepositorInsertsSp-dCas9-2xAM tag
gRNA to be inserted into Bbs I sites
UseCRISPR and LentiviralTags2xAM tagExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterCBh and U6Available sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6)
Plasmid#70232PurposeChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA- HIS3MX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL4A-pCMV7.1
Plasmid#155022PurposeN-terminal 3xFLAG-tagged CUL4A in a pCMV7.1 vectorDepositorInsertCullin 4A (CUL4A Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Rtn3LΔ6LIR-mNeonGreen
Plasmid#169036PurposeExpress human Rtn3L with 6x LC3-interacting regions mutated in mammalian cellsDepositorInsertRtn3L (RTN3 Human)
UseTagsmNeonGreenExpressionMammalianMutationChanged following LC3-interacting regions of Rtn3…PromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Th3
Plasmid#188064PurposePROSS-designed high-redox potential laccase from Trametes hirsutaDepositorInsertPROSS-designed high-redox potential laccase from Trametes hirsuta
UseTagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation20 mutations, described in publicationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Tv9nL
Plasmid#188069PurposePROSS-designed high-redox potential laccase from Trametes versicolorDepositorInsertPROSS-designed high-redox potential laccase from Trametes versicolor
UseTagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation79 PROSS mutations, described in publicationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L309-myc-seipin-WT
Plasmid#64061Purposemyc-tagged murine seipin (wild type); lentiviral constructDepositorInsertbscl2 (Bscl2 Mouse)
UseLentiviralTagsmyc epitopeExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-LSL-nlsGFP-shRNA
Plasmid#175584PurposeConditional express shRNA with control of LoxPDepositorTypeEmpty backboneUseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEFSAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pECE M2-SNX17 WT
Plasmid#69811PurposeExpresses M2-SNX17 in mammalian cellsDepositorInsertSorting nexin-17 (SNX17 Human)
UseTagsFLAGExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔKinase
Plasmid#163404PurposeExpresses BMPR-2 lacking the kinase domain in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe kinase domain (202-500 aa) is deletedPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH4169
Plasmid#200108PurposePrig-3 FRT let858 (stop) FRT snb-1::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of snb GFPDepositorInsertsnb-1 (snb-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPrig-3Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
BII-ChPtW-EGFP
Plasmid#133400PurposePiggybac vector for constitutive expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL7-pCMV7.1
Plasmid#155025PurposeN-terminal 3xFLAG-tagged CUL7 in a pCMV7.1 vectorDepositorInsertCullin 7 (CUL7 Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2xFLAGhYAP2
Plasmid#17793DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationPromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hPGK-eGFP-SynaptoZip
Plasmid#122523PurposeLentiviral expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by hPGK promoterDepositorInserteGFP-SynaptoZip (Vamp2 Rat)
UseLentiviralTagsMyc and eGFPExpressionMammalianMutationPromoterhPGKAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCosSAD1AprR
Plasmid#190786PurposeAn E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombinationDepositorInsertsupstream region of the aur1 cluster for auricin
ampramycin resistance cassette
downstream region of the aur1 cluster for auricin
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-SAM-hygro
Plasmid#138955PurposeLentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance geneDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterU6 AND EF1aAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only