58,039 results

1. pET-28a(+)-FGF2-K128N

   Plasmid

   #120284

   Purpose

   Produce human recombinant FGF2 K128N in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Basic fibroblast growth factor 2 (FGF2 Human, Synthetic)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-FEN1

   Plasmid

   #215129

   Purpose

   Expresses mCherry-FEN1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   FEN1 (FEN1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-cCFP

   Plasmid

   #185361

   Purpose

   For mammalian expression of C-term CFP for split-CFP

   Depositor

   Ayaz Najafov

   Insert

   CFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti CMV RasV12 Neo (w108-1)

   Plasmid

   #22259

   Depositor

   Eric Campeau

   Insert

   HRAS G12V (HRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   G12V mutant

   Promoter

   Available since

   Jan. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS405-FlagPEN2/PS1wt

   Plasmid

   #207803

   Purpose

   Yeast integrative vector for expression of FlagPEN2 and Presenilin 1

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Gamma-secretase subunits PEN-2 and presenilin1 wt
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNestin-EGFP

   Plasmid

   #38777

   Depositor

   Wei Cui

   Article

   Noisa et al Stem Cell Rev. 2010 Sep;6(3):438-49.

   Insert

   nestin 2nd intron enhancer with TK mini promoter (NES Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA Flag Lamp2b-HA

   Plasmid

   #71292

   Purpose

   Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   Flag, HA, and Lamp2 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA-FLAG-METTL3

   Plasmid

   #160250

   Purpose

   Express FLAG-METTL3 in human cells

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. tet_pLKO.1_puro_shNRF2 #1

   Plasmid

   #136584

   Purpose

   Expresses an inducible short hairpin targeting human NRF2 sequence

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   shNRF2v1 (NFE2L2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEBG-Pak1 83-149

    Plasmid

    #12214

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Dominant negative Pak1: autoinhibitory domain, aa…

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF2939

    Plasmid

    #144415

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HOXA6 (HOXA6 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHTN-HaloTag_RPA2:1-270

    Plasmid

    #211013

    Purpose

    Mammalian expression of full-length human RPA2. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    RPA2:M1-E270 (RPA2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. TFORF0575

    Plasmid

    #143885

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HOXA11 (HOXA11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-FLAG-MTOR-R2505P

    Plasmid

    #69015

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-R2505P (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Arginine 2505 to Proline

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSSRB069_pAC8-hsDDB1dB

    Plasmid

    #218790

    Purpose

    Insect cell expression vector for 6xHis-TEV-hsDDB1∆B

    Depositor

    Eric Fischer

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    DNA damage-binding protein 1 (DDB1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Insect

    Mutation

    Deleted amino acids 396-705 and replaced with GNG…

    Promoter

    polyhedrin

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-HIPK2

    Plasmid

    #23481

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    HIPK2 (HIPK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pLKO.1 mito-TagRFP-T

    Plasmid

    #174543

    Purpose

    labels mitochondria with TagRFP-T

    Depositor

    Minna Roh-Johnson

    Article

    Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

    Insert

    MTS-tagRFP-T
    

    Use

    Lentiviral

    Tags

    tagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MOM603

    Plasmid

    #133242

    Purpose

    expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

    Depositor

    Shinsuke Niwa

    Article

    Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

    Insert

    kif1a (KIF1A Human)
    

    Use

    Tags

    StrepII tag and mScarlet

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF2997

    Plasmid

    #144473

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #223173

    Purpose

    Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hfCas13d (N2V8)
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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