We narrowed to 18,831 results for: Tec

1. pBW829_pCAG-pExpr-LUT

   Plasmid

   #87554

   Purpose

   Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   EGFP
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. YSBE 559 : pNAT_6xHA

   Plasmid

   #92087

   Purpose

   Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, NAT selection

   Depositor

   Yong-Sun Bahn

   Article

   So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

   Insert

   6xHA
   

   Use

   tagging proteins in cryptococcus neoforman

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Id2-Halo donor

   Plasmid

   #113116

   Purpose

   Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Id2 donor region including homology arms and HaloTag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFLIP38

   Plasmid

   #65616

   Purpose

   FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-edCit-attR1 and attR2-edCer-cMyc

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. YIp211-P30-His-POL30-3'

   Plasmid

   #99546

   Purpose

   Yeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   POL30 (POL30 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Promoter

   POL30

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GGE252

   Plasmid

   #120791

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown_GSY-NotI
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GGE253

   Plasmid

   #120790

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp_GSY-NotI
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSET FLIPE-1m

   Plasmid

   #13540

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207W
   

   Use

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   A207W in binding protein

   Promoter

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-TetR-Rad18UBD

   Plasmid

   #119010

   Purpose

   Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   TetR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 7xNLS-pMJ915v2-sfGFP

    Plasmid

    #88922

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC19/mlcR25-mEGFP

    Plasmid

    #154295

    Purpose

    Fluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)

    Depositor

    Shigehiko Yumura

    Article

    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

    Insert

    25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pST1374-Lb-Cpf1-NLS

    Plasmid

    #107680

    Purpose

    Expresses LbCpf1 in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    LbCpf1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCellFree_G03 S100A1

    Plasmid

    #67048

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    S100A1 (S100A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCfB4132

    Plasmid

    #106127

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFLIP43

    Plasmid

    #65618

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edAFPt9-attR1 and attR2-edCer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mEos2-VASP-5

    Plasmid

    #57433

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    VASP
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. JME4393

    Plasmid

    #129657

    Purpose

    LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLR5-CAG-NLS-tdiRFP670

    Plasmid

    #82621

    Purpose

    Expresses NLS-tdiRFP670 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    NLS-tdiRFP670
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHCKan-yibDp-GST

    Plasmid

    #134592

    Purpose

    Low Phosphate inducible GST

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    Glutathione S-transferase
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6Rosa-CAG-Cas9

    Plasmid

    #118998

    Purpose

    Mammalian expression of Rosa26 sgRNA, Cas9 and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBT362

    Plasmid

    #127844

    Purpose

    clone BE4max-NG CBEs by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    BE4max-NG without deaminase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. V957 pLPS-3' ECFP-mito - Acceptor

    Plasmid

    #11699

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox; Acceptor vector

    Tags

    ECFP-mito

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 26, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. BCJJ340

    Plasmid

    #117502

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pX330A_FokI-1x4

    Plasmid

    #63604

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TAL2368

    Plasmid

    #36779

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    NCOR1-TALEN_Left (Ncor1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pTMS2

    Plasmid

    #87800

    Purpose

    For constructing MBP-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

    Depositor

    Yu-Zhu Zhang

    Article

    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

    Type

    Empty backbone

    Use

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mApple-VASP-5

    Plasmid

    #54958

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    VASP
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pCellFree_G03 SREBF2

    Plasmid

    #67129

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    SREBF2 (SREBF2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pLKO.1P shMOCS3_2

    Plasmid

    #102974

    Purpose

    Suppression of MOCS3

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shMOCS3_2 (MOCS3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLKO.1P shMOCS3_1

    Plasmid

    #102973

    Purpose

    Suppression of MOCS3

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shMOCS3_1 (MOCS3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUC57-8xHis

    Plasmid

    #101699

    Purpose

    8xHis in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    AATG-[8xHis]-AGCC
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCfB5935

    Plasmid

    #106128

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntA_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. YSBE 628 : pHYG_mCherry

    Plasmid

    #92092

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, HYG selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    mCherry
    

    Use

    tagging proteins in cryptococcus neoforman

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCellFree_G03 YY1

    Plasmid

    #67082

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    YY1 (YY1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pCfB2192

    Plasmid

    #67539

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pML2003

    Plasmid

    #193255

    Purpose

    Propionate sensor expressing eYFP reporter

    Depositor

    Lauren Andrews

    Article

    Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

    Inserts

    prpR
    eYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Pprp and Synthetic promoter P6

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA4/TO-2xStrep-BFLF1

    Plasmid

    #162626

    Purpose

    Expresses N-terminal 2x-Strep-tagged BFLF1 in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    BFLF1
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. YSBE 558 : pNAT_4xFLAG

    Plasmid

    #92086

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, NAT selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    4xFlag
    

    Use

    tagging proteins in cryptococcus neoforman

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCoofy47

    Plasmid

    #55188

    Purpose

    Mammalian expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    10x His-Stop-OneStrep-Stop-S-tag-Stop-CBP-Stop-HP…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GGE209

    Plasmid

    #120793

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp-Mfe
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge