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Showing: 4401 - 4450 of 60949 results

  12. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available Since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41732-plasmid-map-sequence-id-210671

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  13. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid
    #220800
    Purpose
    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance
    Depositor
    Elizabeth Kirby
    Article
    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18
    Insert
    Mars1 (Mars1 Mouse)
    Use
    Lentiviral
    Tags
    2xFLAG and T2A-mCherry
    Mutation
    mutation in MetRS to change aa 274 from L to G
    Promoter
    EF1a
    Available Since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220800-plasmid-map-sequence-id-437285

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  18. pPD292 PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-BFP

    Plasmid
    #249156
    Purpose
    Overexpression of PPH-T2A-dCas9-NFZ-P2A-BFP with HBB IVS2 intron in PPH coding sequence and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-mTagBFP2 (HBB S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249156-plasmid-map-sequence-id-496442

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  20. pAAV-CMV-SpCas9D10A nickase

    Plasmid
    #216737
    Purpose
    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMVd1
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216737-plasmid-map-sequence-id-430337

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  21. pAAV-U6-sgCTG-CMV-GFP

    Plasmid
    #216734
    Purpose
    AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    sgCTG
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Promoter
    U6 and CMV
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216734-plasmid-map-sequence-id-430319

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  22. pAAV-nEF-SpCas9D10A nickase

    Plasmid
    #216736
    Purpose
    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    EF-1-alpha core
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216736-plasmid-map-sequence-id-430323

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  24. pSA097 HNRNPK_IVS3-Cas9-BFP

    Plasmid
    #249147
    Purpose
    Overexpression of SpCas9-BFP with HNRNPK IVS3 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    HNRNPK_IVS3-Cas9-TagBFP (HNRNPK S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HNRNPK IVS3 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249147-plasmid-map-sequence-id-496390

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  25. AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

    Plasmid
    #65454
    Purpose
    Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    ChR2(H134R)-EYFP
    Use
    AAV
    Tags
    EYFP
    Mutation
    H134R variant
    Promoter
    hSyn1
    Available Since
    July 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    65454-plasmid-map-sequence-id-436383

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  26. AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

    Plasmid
    #65429
    Purpose
    Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinases
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    ChR2(H134R)-EYFP
    Use
    AAV
    Tags
    EYFP
    Mutation
    H134R variant
    Promoter
    hSyn1
    Available Since
    July 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65429-plasmid-map-sequence-id-110864

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  28. pCDF_AtRaf1/Raf2/RbcX2/BSD2/RbcX1

    Plasmid
    #229510
    Purpose
    Expresses Arabidopsis thaliana chaperones: Raf1,Raf2,RbcX2,BSD2,RbcX1
    Depositor
    Laura Gunn
    Article
    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.
    Inserts
    Rubisco accumulation factor 1 (AT3G04550 Mustard Weed)
    Rubisco accumulation factor 2 (AT5G51110 Mustard Weed)
    RbcX2 (RbcX2 Mustard Weed)
    BSD2
    RbcX1
    Expression
    Bacterial
    Mutation
    N terminus chloroplast transit peptide truncation
    Promoter
    T7
    Available Since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229510-plasmid-map-sequence-id-460710

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  29. pAAV-STAT-CA

    Plasmid
    #195576
    Purpose
    Expresses STAT3 constitutively active mutant
    Depositor
    Kevin Park
    Article
    Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi
    Insert
    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    changed Alanine 662 to Cysteine and Asparagine 66…
    Promoter
    CMV enhancer and promoter
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195576-plasmid-map-sequence-id-383906

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  36. pSA092 CCT3_IVS13-Cas9-BFP

    Plasmid
    #249142
    Purpose
    Overexpression of SpCas9-BFP with CCT3 IVS13 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    CCT3_IVS13-Cas9-TagBFP (CCT3 S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    CCT3 IVS13 intron
    Promoter
    EF1a
    Available Since
    Jan. 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    249142-plasmid-map-sequence-id-496523

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  37. pSA090 EIF4A3_IVS2-Cas9-BFP

    Plasmid
    #249140
    Purpose
    Overexpression of SpCas9-BFP with EIF4A3 IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    EIF4A3_IVS2-Cas9-TagBFP (EIF4A3 S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    EIF4A3 IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    249140-plasmid-map-sequence-id-496522

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  38. pSA144 HBB_IVS2-Cas9-BFP with core cHS4 insulators

    Plasmid
    #249154
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Flanked by cHS4 core insulators. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    HBB_IVS2-Cas9-TagBFP with core cHS4 insulators (HBB S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249154-plasmid-map-sequence-id-496518

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  39. pSA089 AARS1_IVS11-Cas9-BFP

    Plasmid
    #249139
    Purpose
    Overexpression of SpCas9-BFP with AARS IVS11 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    AARS1_IVS11-Cas9-TagBFP (AARS S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    AARS IVS11 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249139-plasmid-map-sequence-id-496514

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  40. pSA095 EEF2_IVS9-Cas9-BFP

    Plasmid
    #249145
    Purpose
    Overexpression of SpCas9-BFP with EEF2 IVS9 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    EEF2_IVS9-Cas9-TagBFP (EEF2 S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    EEF2 IVS9 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249145-plasmid-map-sequence-id-496388

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  41. pSA094 UBA1_IVS20-Cas9-BFP

    Plasmid
    #249144
    Purpose
    Overexpression of SpCas9-BFP with UBA1 IVS20 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    UBA1_IVS20-Cas9-TagBFP (UBA1 S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    UBA1 IVS20 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249144-plasmid-map-sequence-id-496384

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  43. pTRE3G-mouse cGASdeltaN-GFP

    Plasmid
    #186878
    Purpose
    Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Mouse cGAS truncation mutant (148-508 a.a.) with C-terminal GFP fusion (Cgas Mouse, Synthetic)
    Use
    Retroviral
    Tags
    GFP
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186878-plasmid-map-sequence-id-366111

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  45. pJT340 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast

    Plasmid
    #187344
    Purpose
    dCas9 effector fusion for manipulating transcription
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast
    Use
    CRISPR and Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    KRAB WSR7EEE
    Promoter
    pEF1a
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187344-plasmid-map-sequence-id-373294

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  47. pJT242 Lenti pEF-dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast

    Plasmid
    #187327
    Purpose
    dCas9 effector fusion for manipulating transcription
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast
    Use
    CRISPR and Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    KRAB WSR7EEE
    Promoter
    pEF1a
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187327-plasmid-map-sequence-id-373284

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  49. pJT341 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

    Plasmid
    #195540
    Purpose
    dCas9 effector fusion for manipulating transcription
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast
    Use
    CRISPR and Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Promoter
    pEF1a
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    195540-plasmid-map-sequence-id-387166

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Showing: 4401 - 4450 of 60949 results