We narrowed to 13,716 results for: Cas9

1. pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

   Plasmid

   #198750

   Purpose

   Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

   Depositor

   Maarten Merkx

   Article

   van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

   Insert

   SpdCas9 - SmallBiT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   D10A, H840A (nuclease inactivating mutations in &…

   Promoter

   T7

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

   Plasmid

   #98974

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 3 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

   Plasmid

   #198751

   Purpose

   Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

   Depositor

   Maarten Merkx

   Article

   van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

   Insert

   SpdCas9 - LargeBiT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   D10A, H840A (nuclease inactivating mutations in &…

   Promoter

   T7

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN-dCas9-CDA1-UGI

   Plasmid

   #196248

   Purpose

   Plasmid containing promoter of RecA from Xanthomonas oryzae driving expression of dead Cas9, cytosine deaminase CDA1, and glycosylase inhibitor

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   attL4-RecApro-dCas9-CDA1-UGI-attR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   RecA promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

   Plasmid

   #169912

   Purpose

   Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   dCjCas9
   

   Use

   CRISPR

   Tags

   10XGCN4, 2xSV40NLS, and SV40NLS-HA

   Expression

   Mammalian

   Mutation

   D8A, H559A in cjCas9

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. hRad51(K133R)–Cas9(D10A)

   Plasmid

   #125568

   Purpose

   Expresses the Rad51(K133R)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

   Insert

   Rad51(K133R)–Cas9(D10A) (RAD51 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-GfABC1D-SaCas9-WPRE3-pA

   Plasmid

   #203540

   Purpose

   SaCas9 expression in astrocytes

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   SaCas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-SaCas9-U6-sgGria2

   Plasmid

   #124854

   Purpose

   Mutagenesis of Gria2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria2 (Gria2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-nCas9n-P2A-TagBFP2-pA

   Plasmid

   #176026

   Purpose

   Multisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENT

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   nCas9n-P2A-TagBFP2
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD_A-CmYLCV-dCas9-24XGP41

    Plasmid

    #202009

    Purpose

    Moclo level 1 - Module A, Promoter: CmYLCV, Gene: dCas9-24XGP41, Terminator: HSP

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    dCas9-24XGP41
    

    Use

    Synthetic Biology; Moclo level 1 vector

    Tags

    GS linkers

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZDonor-Nm-Cas9-gRNA-hU6

    Plasmid

    #61366

    Purpose

    empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

    Plasmid

    #129530

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Expression

    Mutation

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXGFPC-5 Pxyl-dcas9 (Sth3)

    Plasmid

    #133317

    Purpose

    expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    dcas9 (Streptococcus thermophilus #3)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    xylose

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_RBMX-eA3A-UdgX-HF-nCas9

    Plasmid

    #163548

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-HF-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSH231-EFS-Cas9-BlastR

    Plasmid

    #115146

    Purpose

    Safe harbor site 231 knock-in vector with Cas9-BlastR expression cassette

    Depositor

    Raymond Monnat, Michael Phelps

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    Cas9-T2A-BlastR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRGEN-CMV-VPR-L1-CjCas9 V-D

    Plasmid

    #169913

    Purpose

    Expression of a VPR-dCjCas9 fusion protein to activate gene expression.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    dCjCas9
    

    Use

    CRISPR

    Tags

    2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    D8A, H559A in cjCas9

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3xFLAG-dCas9/MSCV-EGFP

    Plasmid

    #82613

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g

    Insert

    3xFLAG-dCas9
    

    Use

    Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-xCas9RA-P2A-Puro

    Plasmid

    #110871

    Purpose

    Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. p2T-CAG-KKHSaCas9-BlastR

    Plasmid

    #107189

    Purpose

    Confers constitutive expression of KKH SaCas9.

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Insert

    KKH SaCas9 (NEWENTRY )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E782K/N968K/R1015H

    Promoter

    Chicken ß-Actin

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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