We narrowed to 13,911 results for: nts

1. pEXP5-NT/pLux LuxI

   Plasmid

   #193627

   Purpose

   Inducible expression of LuxI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LuxI is codon optimized for E. coli.

   Depositor

   Dora Tang

   Article

   Gonzales et al ChemSystemsChem 2023

   Insert

   LuxI
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Lux promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-DDX43-NT-G46D

   Plasmid

   #134588

   Purpose

   Express human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length (G154D)

   Promoter

   T7

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEXP5-NT-5th R

   Plasmid

   #42572

   Depositor

   Ivan Korendovych

   Article

   Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

   Insert

   mutated calmodulin gene
   

   Use

   Tags

   Polyhistidine (6×His) region, TEV recognition sit…

   Expression

   Bacterial

   Mutation

   changed Phenylalanine 92 to Glutamine acid, Isole…

   Promoter

   T7

   Available since

   March 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEXP5-NT-7th R

   Plasmid

   #42573

   Depositor

   Ivan Korendovych

   Article

   Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

   Insert

   mutated calmodulin gene
   

   Use

   Tags

   Polyhistidine (6×His) region, TEV recognition sit…

   Expression

   Bacterial

   Mutation

   changed Phenylalanine 92 to Glutamine acid, Isole…

   Promoter

   T7

   Available since

   March 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. Sars Cov 2 MPRO R188S mutantSARS-CoV-2 Mpro protease (R188S mutant - clinical mutant); aliases: 3CLpro, 3C-like

   Plasmid

   #204785

   Purpose

   Bacterial expression of Sars Cov 2 MPRO R188S mutant

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R188S

   Promoter

   Available since

   Jan. 23, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

   Plasmid

   #163169

   Purpose

   Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   Non-targeting
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1 Blast NT shRNA

   Plasmid

   #123306

   Purpose

   Expression of a non-targeting shRNA

   Depositor

   Giovanni Tonon

   Article

   Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

   Insert

   Non-targeting shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCLP-2/NT-IgG (104)

   Plasmid

   #24423

   Depositor

   Steven Rosen

   Article

   Sassetti et al J Biol Chem. 2000 Mar 24. 275(12):9001-1

   Insert

   PCLP-2 (PODXL2 Human)
   

   Use

   Tags

   Human IgG1 Fc

   Expression

   Mammalian

   Mutation

   N-terminal (acidic) domain of PCLP-2 (1-630 bp) f…

   Promoter

   Available since

   Sept. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pM148: pAAV.CMV-Cas13e-NT sgRNA

   Plasmid

   #203446

   Purpose

   Plasmid expressing active RfxCas13d with non-targeting gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-non targeting (NT) sgRNA
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS2702-CMV-iNme2-ABE8e-nt

    Plasmid

    #228723

    Purpose

    Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with iNme2 nCas9 (D16A)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS2703-CMV-iNme2Smu-ABE8e-nt

    Plasmid

    #228724

    Purpose

    Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with iNme2Smu nCas9 (D16A)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD(Nt)_His6-GST-TEV

    Plasmid

    #223878

    Purpose

    4G-cloning donor for N-terminal His6-GST-TEV tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    His6-GST-TEV
    

    Use

    Golden-gate donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD(Nt)_His6-MBP-TEV

    Plasmid

    #223877

    Purpose

    4G-cloning donor for N-terminal His6-MBP-TEV tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    His6-MBP-TEV
    

    Use

    Golden-gate donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pD(Nt)_His6-HA2-TEV

    Plasmid

    #223876

    Purpose

    4G-cloning donor for N-terminal His6-HA2-TEV tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    His6-HA2-TEV
    

    Use

    Golden-gate donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pD(Nt)_His6-FLAG3-TEV

    Plasmid

    #223875

    Purpose

    4G-cloning donor for N-terminal His6-FLAG3-TEV tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    His6-FLAG3-TEV
    

    Use

    Golden-gate donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD(Nt)_His10-TS-3C

    Plasmid

    #223884

    Purpose

    4G-cloning donor for N-terminal His10-TwinStrep-3C tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    His10-TwinStrep-3C
    

    Use

    Golden-gate donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS2700-CMV-Nme2-ABE8e-nt-E932R

    Plasmid

    #228721

    Purpose

    Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with Nme2 nCas9 (D16A) E932R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A E932R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEXP5-NT/6xHis mCherry F30 2xdBroccoli

    Plasmid

    #169233

    Purpose

    Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

    Depositor

    Dora Tang

    Article

    Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

    Insert

    6xHis mCherry F30-2xdBroccoli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSET-dCas9-actII-4-NT-S1

    Plasmid

    #110185

    Purpose

    Repression of gene expression in Streptomyces by CRISPRi

    Depositor

    Yinhua Lu

    Article

    Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

    Inserts

    dCas9
    sgRNA
    

    Use

    CRISPR; I

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    J23119 and ermE*p

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBOBI NT-RFP-PDZD8-CT-GFP

    Plasmid

    #227824

    Purpose

    To amplify the virus that expresses NT-RFP-PDZD8-CT-GFP

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

    Insert

    PDZD8 (PDZD8 Human)
    

    Use

    Lentiviral

    Tags

    GFP and RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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