2,162 results

1. p5E_unc503

   Plasmid

   #64020

   Purpose

   5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45b

   Depositor

   Peter Currie, Joachim Berger

   Article

   Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

   Insert

   muscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   503-bp promoter of zebrafish unc-45b

   Available since

   May 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

   Plasmid

   #105127

   Purpose

   Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein

   Depositor

   Didier Stainier, Kimberley Evason

   Article

   Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. d

   Inserts

   Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
   Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
   

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   cryaa (crystallin) and fabp10a

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUFlip-floxed2A-KalTA4-; HE1A:BFP -0

   Plasmid

   #180010

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; HE1A: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-Rab32b

   Plasmid

   #140885

   Purpose

   Expression of zebrafish GFP-tagged Rab32b in mammalian cells.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rab32b (rab32b Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPRISM-gal4-VP16-gcry-TagBFP

   Plasmid

   #117777

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   gal4/VP16
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MiniCoopR mitfa:BRAF

   Plasmid

   #118846

   Purpose

   Expresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   BRAF V600E (BRAF Human, Zebrafish)
   

   Use

   Zebrafish expression, tol2

   Tags

   Expression

   Mutation

   BRAF V600E

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. th-P2A-EGFP donor

   Plasmid

   #65562

   Purpose

   Intron targeting-mediated EGFP knockin at the zebrafish th locus.

   Depositor

   Jiu-lin Du

   Article

   Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

   Insert

   th-P2A-EGFP (th Zebrafish)
   

   Use

   Zebrafish injection

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGTag-TagRFP-CAAX-SV40

   Plasmid

   #117809

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Tags

   CAAX

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5E-0.349cldn15la

   Plasmid

   #125026

   Purpose

   Five prime entry vector containing 349bp cldn15la promoter from zebrafish. Drives expression in intestinal epithelial cells.

   Depositor

   John Rawls

   Article

   Murdoch et al PLoS Pathog. 2019 Mar 7;15(3):e1007381.

   Insert

   Claudin 15-like a (cldn15la Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCK011 p5E exorh

    Plasmid

    #195949

    Purpose

    Gateway p5E vector

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh regulatory elements
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

    Plasmid

    #180011

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

    Plasmid

    #180012

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2+ Zf GFP-vclb

    Plasmid

    #185411

    Purpose

    GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    vinculin b (vclb Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tol2-lyzC-mcherry-2a-CDK2

    Plasmid

    #121131

    Purpose

    neutrophil specific expression

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    dre-WT CDK2 (cdk2 Zebrafish)
    

    Use

    Tags

    mcherry-2a

    Expression

    Bacterial

    Mutation

    Promoter

    neutrophil specific lyzC

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6a:sgRNA(tyr)

    Plasmid

    #64250

    Purpose

    expresses sgRNA(tyr) under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    U6a:sgRNA (tyr)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. p5E-elavl3

    Plasmid

    #72640

    Purpose

    Gateway p5E 5'entry clone with elavl3 (HuC) enhancer/promoter for pan-neuronal expression in zebrafish

    Depositor

    Joshua Bonkowsky

    Insert

    elavl3 enhancer (elavl3 Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2+ Zf GFP-vcla

    Plasmid

    #185410

    Purpose

    GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    vinculin a (vcla Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hsp70: mKate2-DN Kif5a

    Plasmid

    #105972

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

    Insert

    Kif5a (kif5aa Zebrafish)
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    truncation of aa416-1033

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Gfp-zf-vasa

    Plasmid

    #167323

    Purpose

    Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

    Depositor

    Goro Yoshizaki

    Article

    Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

    Insert

    zf vasa 3'UTRs (ddx4 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST mfap4:icre-p2a-tomato

    Plasmid

    #135217

    Purpose

    Cre coexpressed with tdTomato in macrophages

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    mfap4 promoter with iCre and Tomato
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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