2,159 results

1. pGTag-NLS-TagRFP-SV40

   Plasmid

   #117805

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E_he1a:ECFP

   Plasmid

   #113880

   Purpose

   p3E entry vector for Three Multisite Gateway

   Depositor

   Marnie Halpern

   Insert

   he1a (he1.1 Zebrafish)
   

   Use

   Zebrafish danio expression

   Tags

   ECFP

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Control-cx43.4-48H-pGTag

   Plasmid

   #117818

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Tags

   CAAX

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. zCD2

   Plasmid

   #122031

   Purpose

   Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.

   Depositor

   David Christianson

   Article

   Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

   Insert

   Zebrafish HDAC6 Catalytic Domain 2
   

   Use

   Tags

   His6-MBP tag with cleavable TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP1-sox17 promoter

   Plasmid

   #31400

   Depositor

   Didier Stainier

   Insert

   sox17 promoter (sox17 Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

6. MiniCoopR mitfa:NRAS

   Plasmid

   #118847

   Purpose

   Expresses human NRAS Q61R mutant and zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   NRAS Q61R (NRAS Zebrafish, Human)
   

   Use

   Zebrafish expression, tol3

   Tags

   Expression

   Mutation

   NRAS Q61R

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol2CG2-hsp70I:QFGal4-SV40pA

   Plasmid

   #155120

   Purpose

   Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   hsp70l:QFGal4 (hsp70l Zebrafish, Neurospora crassa, Budding Yeast)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   hsp70l

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUFlip-floxed2A-mRFP-; gcry1:BFP -0

   Plasmid

   #173886

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; gcry1: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p3E-U6a-U6c-rac2 guide

   Plasmid

   #107600

   Purpose

   An entry vector with U6a and U6c promoter driving rac2 guide RNAs expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   rac2 guide RNA sequences
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. shha-pCRII

    Plasmid

    #68962

    Purpose

    for antisense probe, NotI w/ Sp6

    Depositor

    David Traver

    Article

    Clements et al Nature. 2011 Jun 8;474(7350):220-4. doi:

    Insert

    shha (shha Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCK033 p5E desma MCS

    Plasmid

    #195948

    Purpose

    Gateway p5E vector

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    desmin a regulatory elements (desma Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5E-lyzC

    Plasmid

    #107591

    Purpose

    An entry vector with a lyzC promoter

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    Lysozyme C promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Tol2-cmlc2-Trpa1b-2A-EGFP

    Plasmid

    #106405

    Purpose

    For expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.

    Depositor

    Randall Peterson

    Article

    Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

    Inserts

    cmlc2 promoter
    Trpa1b (trpa1b Zebrafish)
    

    Use

    Gateway

    Tags

    2A-EGFP

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. ZE11 tiggywinkle hedgehog CMV

    Plasmid

    #17060

    Depositor

    Randall Moon

    Insert

    tiggywinkle hedgehog (shhb Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pHR_PGK_antiCD19_eZ3_Gal4VP64

    Plasmid

    #169917

    Purpose

    Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

    Depositor

    Zhili Rong

    Article

    Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

    Insert

    antiCD19-Notch3-GL4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Additional EGF repeat between the extracellular d…

    Promoter

    PGK

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2+Paxillin-mKate

    Plasmid

    #105974

    Purpose

    in vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions

    Depositor

    Caren Norden

    Article

    Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

    Insert

    Paxillin
    

    Use

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCK069 desmaMCS:minprom_mApple,exorh:EGFP

    Plasmid

    #195982

    Purpose

    Expression construct

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    desmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP (desma Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTol2CG2-acta1:QFGal4-SV40pA

    Plasmid

    #155121

    Purpose

    Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    acta1:QFGal4
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    acta1

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

    Plasmid

    #191500

    Purpose

    GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye

    Depositor

    Michael Granato

    Article

    Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

    Insert

    CaSR-EGFP-SV40
    

    Use

    Creation of transgenic fish using tol2 transgenes…

    Tags

    Expression

    Mutation

    Promoter

    myo6b

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDestTol2CG2--3.5ubb:plin2-tdtomato

    Plasmid

    #172534

    Purpose

    Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    -3.5ubb:plin2-tdtomato
    

    Use

    Tags

    Expression

    Mutation

    S80R, A81S, E144D, I148F and R415H mutations obse…

    Promoter

    zebrafish ubb

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge