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pFLAG ARMS

Plasmid

#24086

Depositor

Moses Chao

Article

Kong et al J Neurosci. 2001 Jan 1. 21(1):176-85.

Insert

ARMS (Kidins220 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.0 FLAG TRPV4

Plasmid

#45751

Depositor

Robert Lefkowitz

Article

Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

Insert

TRPV4 (Trpv4 Rat)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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VV229: GCaMP6f in fck

Plasmid

#58514

Purpose

Expresses GCaMP6f under the a-CamKII promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

GCaMP6f

Use

Lentiviral

Tags

C-terminal FCYENEV (ER export motif); this was no…

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pRS426GFP-2×PH(PLCδ)

Plasmid

#36092

Depositor

Scott Emr

Article

Stefan et al Mol Biol Cell. 2002 Feb;13(2):542-57.

Insert

2xPH(PLCdelta) fusion

Tags

GFP

Expression

Yeast

Mutation

contains aa11-140 of PLCδ (including the PH domai…

Promoter

PRC1

Available Since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK7 K222R

Plasmid

#80887

Purpose

mammalian expression of ALK7 K222R

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

ALK7 (Acvr1c Rat)

Tags

HA

Expression

Mammalian

Mutation

K222R

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA TrkB

Plasmid

#24088

Depositor

Moses Chao

Article

Yano et al J Neurosci. 2001 Feb 1. 21(3):RC125.

Insert

TrkB (Ntrk2 Rat)

Expression

Mammalian

Mutation

R133I

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-KR

Plasmid

#8985

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

K100R, kinase-inactive

Available Since

Jan. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pLV-Ftractin-mCherry

Plasmid

#85131

Purpose

lentiviral expression of an F-actin live-cell marker (mCherry)

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)

Use

Lentiviral

Tags

mCherry

Promoter

EF1a

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBAC-ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s

Plasmid

#200400

Purpose

Expresses DsRed broadly, expresses QF2 and GCaMP6s in OSNs of the clonal raider ant

Depositor

Daniel Kronauer

Article

Hart et al Cell. 2023 Jul 6;186(14):3079-3094.e17.

Inserts

Discosoma red fluorescent protein
Q factor 2
GCaMP6s

Expression

Insect

Promoter

15x QUAS, ObirOrco (Oocearaea biroi Orco promoter…

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSEP-LAMP1-RFP

Plasmid

#167970

Purpose

Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

Depositor

Juan Bonifacino

Article

Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

LAMP1 (Lamp1 Rat)

Tags

SEP and mRFP

Expression

Mammalian

Mutation

D50E

Promoter

CMV

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV GFAP jGCaMP7b

Plasmid

#171118

Purpose

Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

Depositor

Thomas Oertner

Article

Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

Insert

jGCaMP7b

Use

AAV

Tags

6 His Tag, T7 Tag, and Xpress Tag

Expression

Mammalian

Promoter

GFAP

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-2dV-Camui (T305D/T306D)

Plasmid

#220368

Purpose

T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui

Depositor

Ryohei Yasuda

Article

Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

Insert

mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Synthetic, Rat)

Tags

mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

Expression

Mammalian

Mutation

changed both Threonine 305 and 306 to aspartic ac…

Promoter

CAG

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-SEP-GluR1

Plasmid

#24000

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

GluR1 (Gria1 Rat)

Tags

GluR1 signal peptide (aa1-22) and SEP (supereclip…

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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CMV-LAMP1-HaloTag

Plasmid

#164209

Purpose

Expresses HaloTag tagged LAMP1 under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

LAMP1 (Lamp1 Rat)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-2dV-Camui (T286A)

Plasmid

#220367

Purpose

T286A phosphodead mutant of pCAG-2dV-Camui

Depositor

Ryohei Yasuda

Article

Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

Insert

mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T286A)-mEGFP(A206K) (Camk2a Synthetic, Rat)

Tags

mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

Expression

Mammalian

Mutation

changed Threonine 286 to Alanine

Promoter

CAG

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-EPSIN 1

Plasmid

#22228

Depositor

Pietro De Camilli

Article

Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

Insert

Epsin 1 (Epn1 Rat)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

please see depositor comment below

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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cacna2d1 pcDNA3

Plasmid

#206103

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit

Depositor

Annette Dolphin

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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rGluA2 (px330:2-guideCas9)

Plasmid

#169439

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.iGluSnFR3.v857.PDGFR

Plasmid

#178329

Purpose

Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Has Service

AAV1

Insert

pAAV hSyn iGluSnFR3 v857.PDGFR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and PDGFR TM Domain

Expression

Mammalian

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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CGBE1-NG (pBM1246)

Plasmid

#140256

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)_NG-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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