-
Plasmid#187084PurposeMedium expression of Complex I with subunit N-HA taggedDepositorInsertnuoA-N
UseTagsHA tag nuoNExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD33(EFG)
Plasmid#187087PurposeMedium expression of subunits EFGDepositorInsertnuoEFG
UseTagsHA tag nuoGExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_WTDHFR_WTTS
Plasmid#191282PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationWT DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAW209
Plasmid#113188PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR arrayDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB172a
Plasmid#156324PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBSPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSPK1
Plasmid#18876DepositorInsertpolyphosphate kinase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
PAS513 (TJF080)
Plasmid#65580PurposepETDuet-1 Containing FadD S379RDepositorInsertFadD
UseTagsExpressionBacterialMutationS379RPromoterT7 lacOAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS529 (TJF072)
Plasmid#65603PurposepETDuet-1 Containing FadD Q338R-HisDepositorInsertFadD
UseTags6x HisExpressionBacterialMutationQ338RPromoterT7 lacOAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS504 (TJF067)
Plasmid#65571PurposepETDuet-1 Containing FadD F447SDepositorInsertFadD with F447S mutation
UseTagsExpressionBacterialMutationF447SPromoterT7 lacOAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGU06
Plasmid#212148PurposePlasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.DepositorInsertUseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_R127ATS
Plasmid#191312PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_Q33STS
Plasmid#191289PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationD27N DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_Q33STS
Plasmid#191292PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F/G121V DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_R166QTS
Plasmid#191311PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_WTTS
Plasmid#191310PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_Q33STS
Plasmid#191313PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YG121VDHFR_Q33STS
Plasmid#191293PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y/G121V DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_WTDHFR_R166QTS
Plasmid#191294PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationWT DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_R166QTS
Plasmid#191295PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationD27N DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FDHFR_R166QTS
Plasmid#191296PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_G121VDHFR_R166QTS
Plasmid#191297PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationG121V DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YG121VDHFR_R166QTS
Plasmid#191299PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y/G121V DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_WTDHFR_R127ATS
Plasmid#191300PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationWT DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_R127ATS
Plasmid#191301PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationD27N DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FDHFR_R127ATS
Plasmid#191302PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_G121VDHFR_R127ATS
Plasmid#191303PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationG121V DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YG121VDHFR_R127ATS
Plasmid#191304PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y/G121V DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_R127ATS
Plasmid#191305PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F/G121V DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_WTTS
Plasmid#191283PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationD27N DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FDHFR_WTTS
Plasmid#191284PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_G121VDHFR_WTTS
Plasmid#191285PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationG121V DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_WTTS
Plasmid#191286PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F/G121V DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YG121VDHFR_WTTS
Plasmid#191287PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y/G121V DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_WTDHFR_Q33STS
Plasmid#191288PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationWT DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FDHFR_Q33STS
Plasmid#191290PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_G121VDHFR_Q33STS
Plasmid#191291PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationG121V DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKP1193
Plasmid#167614PurposeExpress TonB H20NDepositorInserttonB H20N
UseTagsExpressionBacterialMutationtonB with H20N mutationPromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW181
Plasmid#113127PurposeE. coli expression vector for His-MBP-E. coli Cas1 R138ADepositorInsertCas1
UseTags6xHis-MBPExpressionBacterialMutationR138APromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW210
Plasmid#113189PurposeE. coli expression vector for untagged E. coli Cas1 H208A + Cas2 + CRISPR arrayDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationH208APromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAS531 (TJF074)
Plasmid#65605PurposepETDuet-1 Containing FadD V4F, W5L -HisDepositorInsertFadD
UseTags6x HisExpressionBacterialMutationV4F, W5LPromoterT7 lacOAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS525 (TJF092)
Plasmid#65595PurposepETDuet-1 Containing FadD Q339GDepositorInsertFadD
UseTagsExpressionBacterialMutationQ339GPromoterT7 lacOAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS509 (TJF032 L448S)
Plasmid#65576PurposepETDuet-1 Containing FadD L448SDepositorInsertFadD
UseTagsExpressionBacterialMutationL448SPromoterT7 lacOAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS532 (TJF075)
Plasmid#65606PurposepETDuet-1 Containing FadD F447S-HisDepositorInsertFadD
UseTags6x HisExpressionBacterialMutationF447SPromoterT7 lacOAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS514 (TJF081)
Plasmid#65581PurposepETDuet-1 Containing FadD F447DDepositorInsertFadD
UseTagsExpressionBacterialMutationF447DPromoterT7 lacOAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS534 (TJF077)
Plasmid#65608PurposepETDuet-1 Containing FadD D372G-HisDepositorInsertFadD
UseTags6x HisExpressionBacterialMutationD372GPromoterT7 lacOAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS535 (TJF078)
Plasmid#65609PurposepETDuet-1 Containing FadD Y9H-HisDepositorInsertFadD
UseTags6x HisExpressionBacterialMutationY9HPromoterT7 lacOAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS530 (TJF073)
Plasmid#65604PurposepETDuet-1 Containing FadD H376R-HisDepositorInsertFadD
UseTags6x HisExpressionBacterialMutationH376RPromoterT7 lacOAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS520 (TJF087)
Plasmid#65587PurposepETDuet-1 Containing FadD Q338GDepositorInsertFadD
UseTagsExpressionBacterialMutationQ338GPromoterT7 lacOAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS503 (TJF066)
Plasmid#65570PurposepETDuet-1 Containing FadD V4F, W5LDepositorInsertFadD with V4F, W5L mutations
UseTagsExpressionBacterialMutationV4F, W5LPromoterT7 lacOAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS515 (TJF082)
Plasmid#65582PurposepETDuet-1 Containing FadD F447RDepositorInsertFadD
UseTagsExpressionBacterialMutationF447RPromoterT7 lacOAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only