We narrowed to 9,389 results for: Coli
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Plasmid#239572PurposeExpresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.DepositorInsert4-aminobutyrate aminotransferase (gabT E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SSDH
Plasmid#239573PurposeExpresses E. coli succinate semialdehyde dehydrogenase (SSDH/gabD) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.DepositorInsertSuccinate-semialdehyde dehydrogenase (gabD E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceJune 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEL2196
Plasmid#170400PurposeFor expresson of LetA-[LetB PLL6->1] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ702-717, replaced with 114-130)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2197
Plasmid#170401PurposeFor expresson of LetA-[LetB PLL6->3] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ702-716, replace with 347-360)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2198
Plasmid#170402PurposeFor expresson of LetA-[LetB PLL6->5] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ702-716, replace with 580-600)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2251
Plasmid#170403PurposeFor expresson of LetA-[LetB PLL6->2] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ700-717, replace with 225-249)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2252
Plasmid#170404PurposeFor expresson of LetA-[LetB PLL6->4] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ700-716, replace with 455-479)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2253
Plasmid#170405PurposeFor expresson of LetA-[LetB PLL6->7] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ700-717, replace with 812-836)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pNB200
Plasmid#169111PurposeTESEC backbone for target expressionDepositorInsertlacZ (lacZ Escherichia coli K12 MG1655)
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E. coliPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mCherry (KmR)
Plasmid#177207PurposePlasmid with p15A origin for constitutive mCherry expression in E. coliDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterSynthetic constitutive promoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
EcMscL
Plasmid#92418PurposeE. coli Mechanosensitive Channel of Large ConductanceDepositorInsertMscL (mscL E. coli)
UseTags6x Histidine Tag and Thrombin Cleavage Site betwe…ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
p416-GPD-HA-PPK
Plasmid#183943PurposeExpresses HA-tagged E. coli PPK from yeast GPD promoterDepositorInsertPPK (ppk E. coli)
UseTagsHAExpressionYeastMutationWTPromoterGPDAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mCherry (ErmR)
Plasmid#177206PurposePlasmid with p15A origin for constitutive mCherry expression in E. coliDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterSynthetic constitutive promoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_1TJE
Plasmid#234203PurposeHeterologous protein expression of threonine--tRNA ligase (EC 6.1.1.3) (Threonyl-tRNA synthetase) in Escherichia coliDepositorInsert1TJE
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
p416-GPD-PPK(mutant)
Plasmid#183942PurposeExpresses catalytic mutant of E. coli PPK from yeast GPD promoterDepositorInsertPPK(mutant) (ppk E. coli )
UseTagsExpressionYeastMutationmutations in His435, His454, and His592 to AlaninePromoterGPDAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK649
Plasmid#72399PurposeProduces Escherichia coli phosphoribosylpyrophosphate amidotransferase (EcPurF)DepositorInsertamidophosphoribosyltransferase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-zwf
Plasmid#65825Purposezwf targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertzwf guide
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-udhA
Plasmid#65818PurposeudhA targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertudhA guide
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA2
Plasmid#65817PurposegltA targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgltA2 guide
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1914
Plasmid#53042PurposePconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetRDepositorInsertsGFP
tetR
UseSynthetic BiologyTagsRBS(st3) and hfqleadExpressionBacterialMutationPromoterPconNoHindM2 and pLlacO-1Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK302
Plasmid#87768PurposeAs pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be usedDepositorInsertsPrhaBAD-sfGFP
rhaS from E. coli with its native RBS from E. coli
UseSynthetic BiologyTags6xHis TagExpressionBacterialMutationPromoterPrhaBAD rhamnose-inducible promoter from E. coli …Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
psgRNA
Plasmid#114005Purposeexpress sgRNA. ColE1, KanDepositorInsertgRNA
UseTagsExpressionMutationPromoterBBa_J23119Available sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB172a
Plasmid#156324PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBSPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
TGT-His
Plasmid#138201PurposeExpresses E. Coli TGT with a C-terminal His TagDepositorInsertE Coli tRNA Guanine Transglycosylase-His
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBIS
Plasmid#17817DepositorInsertmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
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psgRNAc
Plasmid#114006Purposeexpress sgRNA, p15A, CRMDepositorInsertgRNA
UseTagsExpressionMutationPromoterBBa_J23119Available sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
PIA900
Plasmid#104401PurposePolycistronic vector for expression of E. coli core RNAPDepositorInsertrpoA, B, C, and Z
UseE. coli expression vectorTagsencodes a C-terminally His-tagged rpoC which can …ExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMBI
Plasmid#17816DepositorInsertmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-FRT
Plasmid#183241Purposeanhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda RedDepositorInsertsgRNA-FRT
UseTagsExpressionBacterialMutationPromoterP-tetAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PAS528 (TJF059)
Plasmid#65602PurposepETDuet-1 Containing FadD-HisDepositorInsertFadD
UseTags6x HisExpressionBacterialMutationPromoterT7 lacOAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_R166QTS
Plasmid#191298PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F/G121V DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLA230
Plasmid#11724DepositorInsertb-lactamase
UseTagsExpressionBacterialMutationGAA for Glu26 changed to TAA (premature stop codo…PromoterAvailable sinceAug. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTrcECT
Plasmid#75477PurposeInducible expression of ectABC in E. coliDepositorInsertectABC
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAM-RecG-ST2TaV-EGFP
Plasmid#31275DepositorInsertRecG-ST2-TaV-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
EcYbbN pET15b
Plasmid#60690PurposeRecombinant protein expression of YbbNDepositorInsertYbbN
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMAM-RecG-EGFP
Plasmid#31274DepositorInsertRecG-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
MetNI (Lm)
Plasmid#78316PurposeMethionine ABC TransporterDepositorInsertsMetI
MetN
UseTags10x Histidine Tag and 6x Histidine TagExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD33(A-N)
Plasmid#187084PurposeMedium expression of Complex I with subunit N-HA taggedDepositorInsertnuoA-N
UseTagsHA tag nuoNExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD33(EFG)
Plasmid#187087PurposeMedium expression of subunits EFGDepositorInsertnuoEFG
UseTagsHA tag nuoGExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_WTDHFR_WTTS
Plasmid#191282PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationWT DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAW209
Plasmid#113188PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR arrayDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSPK1
Plasmid#18876DepositorInsertpolyphosphate kinase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
PAS513 (TJF080)
Plasmid#65580PurposepETDuet-1 Containing FadD S379RDepositorInsertFadD
UseTagsExpressionBacterialMutationS379RPromoterT7 lacOAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS529 (TJF072)
Plasmid#65603PurposepETDuet-1 Containing FadD Q338R-HisDepositorInsertFadD
UseTags6x HisExpressionBacterialMutationQ338RPromoterT7 lacOAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS504 (TJF067)
Plasmid#65571PurposepETDuet-1 Containing FadD F447SDepositorInsertFadD with F447S mutation
UseTagsExpressionBacterialMutationF447SPromoterT7 lacOAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGU06
Plasmid#212148PurposePlasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.DepositorInsertUseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_R127ATS
Plasmid#191312PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_Q33STS
Plasmid#191289PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationD27N DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only