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We narrowed to 4,562 results for: TIL

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Showing: 401 - 450 of 4562 results

  1. pCAGGS_m-AKAR5’

    Plasmid
    #114140
    Purpose
    Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5' C-terminally tagged by KRas C tail
    Expression
    Mammalian
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114140-plasmid-map-sequence-id-215469

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  2. pCDNA3_altPKA-Cα-mPAGFP

    Plasmid
    #114133
    Purpose
    Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    Testis only splice variant of mouse PKA catalytic subunit α that has no myristoylation site, C-terminally tagged by mPAGFP (Prkaca Mouse)
    Expression
    Mammalian
    Available Since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    114133-plasmid-map-sequence-id-225608

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  4. pSEVA251_ Ptrc.x.tetO2::D-HicDH

    Plasmid
    #185456
    Purpose
    CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.
    Depositor
    Wolfgang Kroutil
    Article
    Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.
    Insert
    D-HicDH from Lactobacillus paracasei DSM 20008
    Expression
    Bacterial
    Promoter
    Ptrc.x.tetO2
    Available Since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185456-plasmid-map-sequence-id-367933

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  5. pLC-Flag-SENP8 sg2R-P2A-Hygro

    Plasmid
    #124300
    Purpose
    Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.
    Depositor
    Eva Gottwein
    Article
    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.
    Insert
    SENP8 (SENP8 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    several silent mutations to disrupt sgRNA targeti…
    Promoter
    CMV
    Available Since
    July 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    124300-plasmid-map-sequence-id-242478

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  7. pCAGGS_m-AKAR5

    Plasmid
    #114139
    Purpose
    Mammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5 C-terminally tagged by KRas C tail
    Expression
    Mammalian
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114139-plasmid-map-sequence-id-215468

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  8. pCAGGS_cyt-AKAR5

    Plasmid
    #114138
    Purpose
    Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5 with C-terminal nuclear export signal (NES)
    Expression
    Mammalian
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114138-plasmid-map-sequence-id-215467

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  23. pCDNA3_nmPKA-CαN-mPAGFP

    Plasmid
    #114130
    Purpose
    Mammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP, with G2A mutation (Prkaca Mouse)
    Expression
    Mammalian
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    114130-plasmid-map-sequence-id-224234

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  24. pDRM209 LTP (Luciferase tdTomato Puro)

    Plasmid
    #174723
    Purpose
    Expresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.
    Depositor
    Wayne Tilley
    Article
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    tdTomato
    pac
    Use
    Lentiviral and Luciferase
    Tags
    E2A linker and T2A linker
    Expression
    Mammalian
    Promoter
    MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    174723-plasmid-map-sequence-id-348966

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  25. pDRM166 LKB (Luciferase mKate2 Blast)

    Plasmid
    #183502
    Purpose
    Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.
    Depositor
    Wayne Tilley
    Article
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    mKate2
    BSD
    Use
    Lentiviral and Luciferase
    Tags
    P2A linker and SV40 NLS with GGS linker
    Expression
    Mammalian
    Promoter
    MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    183502-plasmid-map-sequence-id-362519

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  27. pDRM18 LTN (Luciferase tdTomato Neo)

    Plasmid
    #174721
    Purpose
    Expresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.
    Depositor
    Wayne Tilley
    Article
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    tdTomato
    aph
    Use
    Lentiviral and Luciferase
    Tags
    E2A linker and P2A linker
    Expression
    Mammalian
    Promoter
    MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    174721-plasmid-map-sequence-id-349021

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  35. pCDNA3_shRNA resistant nmPKA-Cα-mEGFP

    Plasmid
    #114144
    Purpose
    The G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    mouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
    Expression
    Mammalian
    Mutation
    G2A mutation and silent mutation to resist shRNA …
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114144-plasmid-map-sequence-id-224274

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  37. pDRM210 LGP (Luciferase GFP Puro)

    Plasmid
    #174722
    Purpose
    Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene
    Depositor
    Wayne Tilley
    Article
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    eGFP
    pac
    Use
    Lentiviral and Luciferase
    Tags
    E2A linker and F2A linker
    Expression
    Mammalian
    Promoter
    MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    174722-plasmid-map-sequence-id-348963

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Showing: 401 - 450 of 4562 results