We narrowed to 223 results for: stem loop

1. pCI-MS2V5-eIF4G delN83

   Plasmid

   #65812

   Purpose

   expression of eIF4G-MS2 fusion.

   Depositor

   Niels Gehring

   Article

   Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

   Insert

   eIF4G (EIF4G1 Human)
   

   Tags

   MS2 stem loops and V5

   Expression

   Mammalian

   Mutation

   delN83 (functionally behaves as the WT protein)

   Promoter

   CMV

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-MS2V5-eRF3a F76a

   Plasmid

   #65811

   Purpose

   expression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1

   Depositor

   Niels Gehring

   Article

   Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

   Insert

   eRF3a (GSPT1 Human)
   

   Tags

   MS2 stem loops and V5

   Expression

   Mammalian

   Mutation

   F76a

   Promoter

   CMV

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ141-ZmUbi-RZ-Aa3.8.4

   Plasmid

   #136375

   Purpose

   Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. NG1301 pCI-TPI-4MS2-SMG5(short)-4H

   Plasmid

   #65806

   Purpose

   NMD reporter mRNA

   Depositor

   Niels Gehring

   Article

   Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

   Insert

   TPI (TPI1 Human)
   

   Tags

   4 MS2 stem loops, SMG5 3'UTR shortened versi…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA436

   Plasmid

   #216035

   Purpose

   Fragmid fragment: (Cas protein) binds MS2 stem loops

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   MS2_v1.1
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ141-ZmUbi-RZ-Aa3.8.3

   Plasmid

   #136374

   Purpose

   Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ141-ZmUbi-RZ-Aac.3

   Plasmid

   #136377

   Purpose

   Gateway entry clone for AacCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold with MS2 at the third stem loop is used

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ141-ZmUbi-RZ-Aa1.2.3

   Plasmid

   #136372

   Purpose

   Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 with MS2 at the third stem loop is used

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-sgRNA(MS2)

   Plasmid

   #102560

   Purpose

   Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJR101

    Plasmid

    #187241

    Purpose

    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    

    Use

    Lentiviral

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJR100

    Plasmid

    #187240

    Purpose

    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expression

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid

    #119946

    Purpose

    Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Renilla luciferase
    

    Tags

    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

    Expression

    Bacterial and Mammalian

    Promoter

    TetCMV

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTET2-RlucCP-3MS2

    Plasmid

    #198354

    Purpose

    Part of Renilla reporter system, encodes Renilla luciferase with MS2 stem loops in the 3'UTR

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    Renilla Luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    pTET2

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTET2-lucCP-3MS2

    Plasmid

    #198351

    Purpose

    Part of Firefly reporter system, encodes Firefly luciferase with MS2 stem loops in the 3'UTR

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    Firefly Luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    pTET2

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AA020

    Plasmid

    #216004

    Purpose

    Fragmid fragment: (Cas protein) binds PP7 stem loops

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    PP7 Core Protein (PCP)_v1.1
    

    Use

    CRISPR; Fragment

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase

    Plasmid

    #191481

    Purpose

    Luciferase reporter for SARS-CoV-2 5' UTR ΔSL1

    Depositor

    Hao Wu

    Article

    Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

    Insert

    SARS-CoV-2 5' UTR ΔSL1
    

    Use

    Lentiviral and Luciferase

    Mutation

    Missing the stem-loop 1 region of SARS-CoV-2 5&#x…

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-ihSyn1-tTA

    Plasmid

    #99120

    Purpose

    An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    tTA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    ihSyn1

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. A3Bctd-L7G-Cas9n-UGI-NLS

    Plasmid

    #198886

    Purpose

    Express A3Bctd-L7G BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Loop 7 of A3G

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHES 946

    Plasmid

    #112644

    Purpose

    blue light inducible cryptochrome based optogenetic expression system

    Depositor

    Hana El-Samad

    Article

    Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

    Insert

    RTG3AD-GSx7-CIB1-SV40NLS (CIB1 Mustard Weed, Synthetic, Budding Yeast)
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-ihSyn1-DIO-tTA

    Plasmid

    #99121

    Purpose

    An AAV genome with Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTA

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    DIO-tTA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    ihSyn1

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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