We narrowed to 4,132 results for: pCMV mammalian

1. LbCas12a-niCPE

   Plasmid

   #213747

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-nLbCas12a-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, R1138A in LbCas12a

   Promoter

   CMV

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP

   Plasmid

   #121448

   Purpose

   To make a stable human recombinant cell line; see right thumbnail map image for PQR annotations

   Depositor

   Brian Chen

   Article

   Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

   Insert

   RFPnols-PQR-ZeocinR (ACTB Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Gluc-LHCGR

   Plasmid

   #221781

   Purpose

   Measurement of glycoprotein-glycoprotein receptor binding using the BRET phenomenon and measurement of membrane expression of the Gluc-LHCGR

   Depositor

   Adolfo Rivero-Muller

   Article

   Adamczuk et al Endocrinology. 2024 Apr 29;165(6):bqae05

   Insert

   LHCGR (LHCGR Human)
   

   Tags

   Gaussia Luciferase and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKC-4.06

   Plasmid

   #112084

   Purpose

   NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)

   Depositor

   James Inglese

   Article

   Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1

   Insert

   3XFLAG firefly luciferase-beta-globin 39PTC (HBB Photinus pyralis, Human, Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKC-4.04

   Plasmid

   #112085

   Purpose

   Control for NMD sensor: pCMV-3XFLAG-Fluc-betaglobin

   Depositor

   James Inglese

   Article

   Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1

   Insert

   3XFLAG firefly luciferase-beta-globin (HBB Photinus pyralis, Human, Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. peCALWY-4

   Plasmid

   #22236

   Depositor

   Maarten Merkx

   Article

   Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

   Insert

   eCALWY-4 Zn(II) sensor (ATOX1 Human, GFP's)
   

   Expression

   Mammalian

   Mutation

   C418S mutation linker consists of 9 GGSGGS repea…

   Available Since

   Nov. 25, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LbCas12a-nuCPE

   Plasmid

   #213748

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-nuCPE in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-LbCas12a-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R in LbCas12a

   Promoter

   CMV

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 1127-DP NTP wild type

   Plasmid

   #32398

   Depositor

   Kathleen Green

   Article

   Kowalczyk et al J Cell Biol. 1997 Nov 3;139(3):773-84.

   Insert

   Desmoplakin (DSP Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains only the first 584 aa

   Promoter

   CMV

   Available Since

   Nov. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

9. peCALWY-1

   Plasmid

   #22233

   Depositor

   Maarten Merkx

   Article

   Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

   Insert

   eCALWY-1 Zn(II) sensor (ATOX1 Human, GFP's)
   

   Expression

   Mammalian

   Mutation

   linker consists of 9 GGSGGS repeats

   Available Since

   Nov. 18, 2009

   Availability

   Academic Institutions and Nonprofits only

10. Beta-Globin-PTC39

    Plasmid

    #130701

    Purpose

    Expresses Beta-globin-PTC39 NMD reporter (It has a Premature termination codon (PTC) at 39th Amino acid)

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    Beta Globin (HBB Human)
    

    Expression

    Mammalian

    Mutation

    PTC39

    Promoter

    Pcmv

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SEPT5 WT

    Plasmid

    #27272

    Depositor

    Harish Pant

    Article

    Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

    Insert

    Septin 5 (SEPTIN5 Human)
    

    Tags

    Myc (MASMQKLISEEDL)

    Expression

    Mammalian

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

12. NLS-CanlonicSF

    Plasmid

    #188390

    Purpose

    To explore lactate dynamics in intact systems

    Depositor

    Alejandro San Martín

    Article

    Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

    Insert

    NLS-CanlonicSF
    

    Tags

    NLS and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. peCALWY-6

    Plasmid

    #22238

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY6 Zn(II) sensor (ATOX1 Human, GFP's)
    

    Expression

    Mammalian

    Mutation

    C416S mutation Identical to peCALWY-4, but linke…

    Available Since

    Jan. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

14. peCALWY-5

    Plasmid

    #22237

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-5 Zn(II) sensor (ATOX1 Human, GFP's)
    

    Expression

    Mammalian

    Mutation

    C416S mutation identical to peCALWY-4, but linke…

    Available Since

    Nov. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

15. peCALWY-3

    Plasmid

    #22235

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-3 Zn(II) sensor (ATOX1 Human, GFP's)
    

    Expression

    Mammalian

    Mutation

    Similar to eCALWY-1, but with a linkerlength of 3…

    Available Since

    Nov. 20, 2009

    Availability

    Academic Institutions and Nonprofits only

16. peCALWY-2

    Plasmid

    #22234

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-2 Zn(II) sensor (ATOX1 Human, GFP's)
    

    Expression

    Mammalian

    Mutation

    sequence identical to eCALWY-1, but linkerlength …

    Available Since

    Nov. 20, 2009

    Availability

    Academic Institutions and Nonprofits only

17. HP21-TPI-WT-no_introns

    Plasmid

    #131044

    Purpose

    Expresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTR

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    TPI-Wildtype-no introns (TPI1 Human)
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HP21-TPI-PTC1

    Plasmid

    #131043

    Purpose

    Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid) with a 21 nt hairpin in 5'UTR

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    TPI-PTC1 (TPI1 Human)
    

    Expression

    Mammalian

    Mutation

    PTC1

    Promoter

    Pcmv

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SEPT5 Mut(S161A/S327A)

    Plasmid

    #27275

    Depositor

    Harish Pant

    Article

    Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

    Insert

    Septin 5 (SEPTIN5 Human)
    

    Tags

    Myc (MASMQKLISEEDL)

    Expression

    Mammalian

    Mutation

    S161A and S327A

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

20. cmv 16 E7 del PTLHE

    Plasmid

    #13688

    Depositor

    Karl Munger

    Article

    Gonzalez et al J Virol. 2001 Aug . 75(16):7583-91.

    Insert

    HPV 16 E7 del PTLHE (E7 Human)
    

    Expression

    Mammalian

    Mutation

    del aa 6-10

    Available Since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

21. SEPT5 Mut(S327A)

    Plasmid

    #27274

    Depositor

    Harish Pant

    Article

    Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

    Insert

    Septin 5 (SEPTIN5 Human)
    

    Tags

    Myc (MASMQKLISEEDL)

    Expression

    Mammalian

    Mutation

    S327A

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pPKm-163

    Plasmid

    #104854

    Purpose

    pcDNA3 - pCMV - PIF3 - GBD, expressing PIF3 and GBD, under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    PIF3-GBD
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mycUb-25aa

    Plasmid

    #20256

    Depositor

    Nico Dantuma

    Article

    Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

    Insert

    Ubiquitin-25 amino acid extension (RBX1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Glycine 76 …

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

24. D10A-Cas9

    Plasmid

    #239383

    Purpose

    For circular RNA-mediated inverse prime editor using nCas9-D10A in HEK294T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

    Insert

    nCas9-D10A
    

    Use

    CRISPR

    Tags

    BPNLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A in Cas9

    Promoter

    CMV

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LC3B-TdLanYFP

    Plasmid

    #228565

    Purpose

    Encodes an acceptor-only, TdLanYFP-tagged version of the LC3B biosensor under the control of the CMV promoter

    Depositor

    Giulia Bertolin

    Article

    Gokerkucuk et al Autophagy. 2023 Aug;19(8):2275-2295. doi

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Tags

    TdLanYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Aquamarine-LC3B G120A

    Plasmid

    #228563

    Purpose

    Encodes a donor-only, Aquamarine-tagged version of the inactive LC3B biosensor under the control of the CMV promoter

    Depositor

    Giulia Bertolin

    Article

    Gokerkucuk et al Autophagy. 2023 Aug;19(8):2275-2295. doi

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Tags

    Aquamarine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Aquamarine-LC3B

    Plasmid

    #228559

    Purpose

    Encodes a donor-only, Aquamarine-tagged version of the LC3B biosensor under the control of the CMV promoter

    Depositor

    Giulia Bertolin

    Article

    Gokerkucuk et al Autophagy. 2023 Aug;19(8):2275-2295. doi

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Tags

    Aquamarine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TDP-43 mRuby K84ONBK

    Plasmid

    #216226

    Purpose

    TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.

    Depositor

    Jennifer Lee

    Article

    Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

    Insert

    Transactive response DNA binding protein of 43 kDa (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    Lysine 84 mutated to a TAG stop codon

    Promoter

    CMV Promoter

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mycUb-20aa

    Plasmid

    #20255

    Depositor

    Nico Dantuma

    Article

    Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

    Insert

    Ubiquitin-20 amino acid extension (RBX1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Glycine 76 …

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Flag-Bcl2

    Plasmid

    #18003

    Depositor

    Clark Distelhorst

    Article

    Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

    Insert

    Bcl-2 (BCL2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pU6-AAVS1-5-1kbdonor

    Plasmid

    #234735

    Purpose

    PseCAST crRNA targeting AAVS1-5, with 1 kb donor transposon

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    AAVS1-5 crRNA
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pU6-hROSA26-1-1kbdonor

    Plasmid

    #234736

    Purpose

    PseCAST crRNA targeting hROSA26-1, with 1 kb donor transposon

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    hROSA26-1 crRNA
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. IF-GFP-ATRX

    Plasmid

    #45444

    Purpose

    ATRX expression vector

    Depositor

    Michael Dyer

    Article

    Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

    Insert

    ATRX (ATRX Human, Mouse)
    

    Tags

    GFP and HA

    Expression

    Bacterial and Mammalian

    Mutation

    isoform 2, missing codon E124

    Promoter

    PCMV

    Available Since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MCP-M-MLV RT∆RNase H

    Plasmid

    #213749

    Purpose

    For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    MCP-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. hWIP1 D314A FLAG

    Plasmid

    #28106

    Depositor

    Lawrence Donehower

    Article

    Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-

    Insert

    WIP1 (PPM1D Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains phosphatase-dead mutation D314A

    Available Since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pFX-Pickles 2.34NES

    Plasmid

    #100028

    Purpose

    Improved FRET biosensor for the measurement of BCR-ABL activity.

    Depositor

    Yusuke Ohba

    Article

    Horiguchi et al Cell Struct Funct. 2017 Feb 2;42(1):15-2

    Insert

    Pickles-2.34NES
    

    Use

    Pfx vector is derived from pcmv vector.

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MMLV-RT(dRH) for Split-PE (pEH50)

    Plasmid

    #190106

    Purpose

    Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    MMLV-RT(delta RNAseH)
    

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    mutations from RT in PE2 and truncation of RNAse …

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Flag- MCL1-3A (S159, S162, T163 mutated to A)

    Plasmid

    #197458

    Purpose

    MCL-1 phosphodegron mutants

    Depositor

    Omar Khan

    Article

    Keyan et al Cancers (Basel). 2023 Jan 13;15(2):505.

    Insert

    MCL1 (MCL1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S159A, S161A, T163A

    Promoter

    CMV

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHA-mLRH-1

    Plasmid

    #16342

    Depositor

    Austin Cooney

    Article

    Gu et al Mol Cell Biol. 2005 May . 25(9):3492-505

    Insert

    LRH-1 (Nr5a2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Dec. 6, 2007

    Availability

    Academic Institutions and Nonprofits only

40. pR2Q024y

    Plasmid

    #101022

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    Glutamate receptor subunit (Gria2 Rat)
    

    Tags

    fluorescent protein

    Expression

    Mammalian

    Mutation

    tagged with fluorescent protein

    Promoter

    cmV

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge