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Plasmid#183198PurposeEntry cloneDepositorInsertCas9-HMGB1
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-APEX2-mCherry
Plasmid#188944PurposeMiddle entry vector for Gateway cloning containing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherryDepositorInsertmito-APEX2_p2A_APEX2-H2B_p2A_mCherry
UseMiddle entry vector for gateway (l1-l2)TagsExpressionMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-RFC4
Plasmid#183199PurposeEntry cloneDepositorInsertCas9-RFC4
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH2827
Plasmid#179542PurposePnmr-1::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 RFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagswCherryExpressionWormMutationPromoterPnmr-1Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXW109Hg(RS-RinA)
Plasmid#123150PurposeMercury sensor with two-layered amplifier cascade. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, PmerT, PhrpLE, PrinAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-WIPI2d10-364delta263-295-TEV-STREP
Plasmid#171830PurposeMammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutation10-364delta263-295PromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 K395A/K396A
Plasmid#162656PurposeExpresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acids K395 and K396 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJP72
Plasmid#176479PurposeExpresses mScarlet under control of the Capsaspora EF1 promoter and GenR under control of the Capsaspora actin promoterDepositorInsertsactin > GenR
EF1 > mScarlet
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B L69E
Plasmid#175026PurposeExpresses WIPI2B (L69E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (L69E) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationLeucine 69 to Glutamate (CTA to GAA)PromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH3409
Plasmid#179574PurposePceh-12::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans VB-MN -specific expression of miniSOG-SL2 RFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagswCherryExpressionWormMutationPromoterPceh-12Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (3'UAG)
Plasmid#170128PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(3'UAG)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
acta1-Cavin4a-Clover
Plasmid#163853PurposeZebrafish muscle specific expression of Cavin4a-Clover, Parton lab clone DPVDepositorInsertCavin4a (cavin4a Zebrafish)
UseZebrafish expressionTagsCloverExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXW109ABS84(RS)
Plasmid#123155PurposeAmplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR-ABS84, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
C211
Plasmid#174360PurposeFor expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfectionDepositorInsertPABPC1 (PABPC1 Human)
UseTagsFLAGExpressionMammalianMutationI110L, D111E, D117E, A121G, G139A, Y140F, T147S, …PromoterAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet
Plasmid#83286Purposeuntargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pairDepositorInsertmTurquoise2-FHA2-AURKB substrate-YPet
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-SAC1-HLx8
Plasmid#108139PurposeSAC1 with extended linkerDepositorInsertmCherry:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-520):[EAAAR]8:SACM1L(521-587)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEM01
Plasmid#135414PurposeExpression of Hygromycin resistance from CMV promoterDepositorInsertCMVpr hph ADH1t
UseTagsExpressionBacterial and YeastMutationPromoterCMVprAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
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SMT205
Plasmid#134817PurposeSMT201 with mutated RBS for DHFR, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only