Addgene

We narrowed to 3,011 results for: AER

Showing: 2701 - 2730 of 3011 results

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pLV-4gRNA-GBX2-RFP

Plasmid

#192288

Purpose

Expresses RFP and four sgRNAs against GBX2

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

RFP and four sgRNA GBX2 (GBX2 Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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str-KDEL_Stx5L(M55V)-SBP-mCitrine

Plasmid

#209846

Purpose

Synchronize trafficking of Stx5L (M55V) from the ER, for use with FLIM (RUSH system)

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

STX5 (STX5 Human)

Use

Tags

Streptavidin Binding Protein (SBP) and mCitrine

Expression

Mammalian

Mutation

Changed Methionine 55 to Valine, causes loss of S…

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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str-KDEL_Stx5S-SBP-mCitrine

Plasmid

#154845

Purpose

Synchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system)

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

Stx5 (Short isoform) (STX5 Human)

Use

Tags

Streptavidin Binding Protein (SBP) and mCitrine

Expression

Mammalian

Mutation

Lacks amino acids 1-54, enocdes short isoform of …

Promoter

CMV

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S12TAG

Plasmid

#107155

Purpose

MBP-rPKL construct for production and purification of rat PKL pS12

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Use

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 12 to TAG for phosphoserine insertion

Promoter

PBAD

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S113TAG

Plasmid

#107157

Purpose

MBP-rPKL S113TAG for production and purification of rat PKL pS113

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Use

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 113 changed to TAG for phosphoserine inser…

Promoter

PBAD

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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RALA V25L

Plasmid

#122927

Purpose

Bacterial expression of RALA V25L

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

V25L

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA V25M

Plasmid

#122928

Purpose

Bacterial expression of RALA V25M

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

V25M

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA D130G

Plasmid

#122929

Purpose

Bacterial expression of RALA D130G

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

D130G

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA S157A

Plasmid

#122930

Purpose

Bacterial expression of RALA S157A

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

S157A

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA R176X

Plasmid

#122931

Purpose

Bacterial expression of RALA R176X

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

R176X

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA WT

Plasmid

#122925

Purpose

Bacterial expression of RALA WT

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA G23D

Plasmid

#122926

Purpose

Bacterial expression of RALA G23D

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Use

Tags

His

Expression

Bacterial

Mutation

G23D

Promoter

T7

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAav-IQGAP1-PQS1-3xFLAG

Plasmid

#84885

Purpose

rAAV-based template for genome engineering of the IQGAP1 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Inserts

IQGAP1 genomic region HR1 (IQGAP1 Human)
IQGAP1 genomic region HR2

Use

AAV

Tags

PQS1 3xFLAG

Expression

Mutation

Promoter

no

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAav-TP53-PQS1-3xFLAG

Plasmid

#84884

Purpose

rAAV-based template for genome engineering of the p53 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Inserts

TP53 genomic region HR1 (TP53 Human)
TP53 genomic region HR2

Use

AAV

Tags

PQS1 3xFLAG

Expression

Mutation

Promoter

no and no

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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KHBD00450

Plasmid

#39563

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6103 (CrebB Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00026

Plasmid

#34188

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8333 (E(spl)mgamma-HLH Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00053

Plasmid

#34215

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6103 (CrebB Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
CXCR4-SZ158a QTY

Plasmid

#162442

Purpose

Expression in E coli and perform ligand binding in solution

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Use

Tags

his-tag, Rho-tag

Expression

Bacterial

Mutation

All L, I, V, F in the transmembrane region mutate…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ146a QTY

Plasmid

#162443

Purpose

Expression in E coli and perform ligand binding in solution

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Use

Tags

his-tag, Rho-tag

Expression

Bacterial

Mutation

All L, I, V, F in the transmembrane region mutate…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ158a-sfGFP

Plasmid

#162446

Purpose

Expression in HEK293T cell and compete ligand singaling against full-length receptors

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Use

Tags

sfGFP

Expression

Mammalian

Mutation

truncation from aa52 to aa257

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-WT Neo

Plasmid

#223058

Purpose

METTL1 gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

METTL1 (METTL1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-AlbEx14-mNG

Plasmid

#201780

Purpose

knocks in mNeonGreen into exon 14 of Alb to produce Alb-mNeonGreen fusion protein in mice

Depositor

Hajime Hirase

Article

Vittani et al Fluorescence Imaging of the Brain. Neuro

Inserts

Alb gRNA (Alb Mouse)
Alb_LHA-mNeonGreen-Alb_RHA (Alb Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAav-MDM2-PQS2-3xHA

Plasmid

#84886

Purpose

rAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Inserts

MDM2 genomic sequence HR1 (MDM2 Human)
MDM2 genomic insert HR2 (MDM2 Human)

Use

AAV

Tags

PQS2 3xHA

Expression

Mutation

Promoter

no

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

Plasmid

#162878

Purpose

Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pdest N-FLAG METTL1-WT puro

Plasmid

#223055

Purpose

METTL1-WT gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

METTL1-WT (METTL1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
rag2:LRRC10_CE_pISceI

Plasmid

#223042

Purpose

Express LRRC10 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

LRRC10 (LRRC10 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:BEST3_CE_pISceI

Plasmid

#223043

Purpose

Express BEST3 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

BEST3 (BEST3 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
rag2:CNOT_CE_pISceI

Plasmid

#223046

Purpose

Express CNOT2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

CNOT2 (CNOT2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
rag2:MARCH9_CE_pISceI

Plasmid

#223027

Purpose

Express MARCH--9 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

MARCH--9 (MARCH9 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
rag2:METTL1_CE_pISceI

Plasmid

#223029

Purpose

Express METTL1 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

METTL1 (METTL1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge