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pHAGE-GUCY2C

Plasmid

#116749

Purpose

Lentiviral expression of GUCY2C

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GUCY2C (GUCY2C Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

Plasmid

#123614

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A)-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mScarlet-BlastR [M1G]

Plasmid

#171814

Purpose

Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mScarlet-T2A-BlastR

Use

Gene tagging

Tags

Expression

Mutation

BlastR: Changed Methionin 1 to Glycine.

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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px335 Mettl14 sgRNA #2

Plasmid

#61514

Purpose

encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

Depositor

Jacob Hanna

Article

Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

Insert

gccgctcccggatctcctgc

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
FUW TetO HA-AMPKa2 WT

Plasmid

#69826

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-IDR1-mCherry-Cry2

Plasmid

#177125

Purpose

Express IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (IDR1 domain) (YTHDC1 Human)

Use

Tags

mCherry-CRY2

Expression

Mammalian

Mutation

IDR1 domain only

Promoter

SFFV

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-sgACSL3.C-Cas9-P2A-Puro

Plasmid

#129412

Purpose

Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

SpCas9 and sgACSL3.C (ACSL3 Human)

Use

CRISPR

Tags

P2A-Puro

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa2 AS

Plasmid

#69827

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-ACSL3-sfGFP(C) HDR template

Plasmid

#129413

Purpose

HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

ACSL3 HDR template (ACSL3 Human)

Use

CRISPR and TALEN; Endogenous tagging hdr template

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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px335 Mettl14 sgRNA #1

Plasmid

#61515

Purpose

encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

Depositor

Jacob Hanna

Article

Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

Insert

gcggcagctcctagctcagc

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
BE3(E63Q)-P2A-EGFP (pRZ189)

Plasmid

#123613

Purpose

CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(E63Q)-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

E63Q in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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nCas9-UGI control for Target-AID (pRZ1047)

Plasmid

#131299

Purpose

CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA2028

Plasmid

#97001

Purpose

Expressing MRSA Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRetro-CAG-DsRedExpress-G-TVA

Plasmid

#235698

Purpose

Expresses G protein, TVA receptor, and DsRed for rabies virus trans-synaptic tracing

Depositor

Guo-li Ming, Hongjun Song

Article

Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

Insert

DsRedExpress, TVA800, and Glyco

Use

Retroviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-CIB1-mRuby-Rab35

Plasmid

#194334

Purpose

For use in light-induced Rab35 protein inactivation through the interaction with CRY2

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Inserts

CIB1 (CIB1 Mustard Weed)
Rab35 (RAB35 Human)

Use

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pDR111_mannose_Klf4Gata-3

Plasmid

#188401

Purpose

Expresses mouse transcription factors Klf4 and Gata-3 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Inserts

Klf4 (Klf4 Mouse)
Gata-3 (Gata3 Mouse)

Use

Tags

PhoD secretion peptide

Expression

Bacterial

Mutation

See Depositor Comments Below

Promoter

D-mannose

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-YTH-mCherry-Cry2

Plasmid

#177126

Purpose

Express YTH domain fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (YTH domain) (YTHDC1 Human)

Use

Tags

mCherry-CRY2

Expression

Mammalian

Mutation

YTH domain only

Promoter

SFFV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTXBI-mclover-YTHDC1-FL

Plasmid

#177121

Purpose

Bacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (YTHDC1 Human)

Use

Tags

Mxe GyrA intein-Chitin binding domain for protein…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTW5126

Plasmid

#129732

Purpose

polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein

Depositor

James Fleckenstein

Article

Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do

Insert

eatA-DUF::His8

Use

Tags

9His tag

Expression

Bacterial

Mutation

replaces the domain of unknown function correspon…

Promoter

araBAD

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-GUCY2C-G549C

Plasmid

#116407

Purpose

Lentiviral expression of GUCY2C G549C

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GUCY2C (GUCY2C Human)

Use

Lentiviral

Tags

Expression

Mutation

G549C

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge