We narrowed to 11,638 results for: NSI

1. pcDNA3-Flag-NSF/S647A

   Plasmid

   #74938

   Purpose

   Mammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)

   Depositor

   Elisa Greggio

   Article

   Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

   Insert

   NSF (NSF Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S647A (mutation in the D2 domain of the protein)

   Promoter

   CMV

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pCS2+8-CYP2A6(C14A, C82A, C439A)-flag-tev-halo-his

   Plasmid

   #227164

   Purpose

   For T-REX experiments of CYP2A6 C14A, C82A, and C439A triple mutant. Almost no sensing ability to reactive electrophilic species (RES).

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   CYP2A6 (CYP2A6 Human)
   

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Mutation

   changed cysteine 14, cysteine 82, and cysteine 43…

   Promoter

   CMV and SP6

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Coexpressed Free NES

   Plasmid

   #182482

   Purpose

   Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS
   NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Expression

   Yeast

   Promoter

   GAL10 and GAL7

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA4 3xFLAG Halo E55A E57A K58A nsp1

   Plasmid

   #175427

   Purpose

   Expresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 E55A E57A K58A
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   N-terminal mutants E55A E57A K58A

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

   Plasmid

   #153441

   Purpose

   E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc. XDocB binding mode B knock-out mutant
   

   Tags

   3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

   Expression

   Bacterial

   Mutation

   Arginine 140 and methionine 144 were mutated to a…

   Promoter

   T7 promoter

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJC20cdc8.110

   Plasmid

   #99363

   Purpose

   Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Expression

   Bacterial

   Mutation

   Alanine 18 to Threonine and Glutamic acid 31 to L…

   Promoter

   T7

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Flag-NSF/T646A

   Plasmid

   #74937

   Purpose

   Mammalian expression of human NSF mutant T646A (mutation in the D2 domain of the protein)

   Depositor

   Elisa Greggio

   Article

   Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

   Insert

   NSF (NSF Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T646A (mutation in the D2 domain of the protein)

   Promoter

   CMV

   Available Since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-Flag-NSF/T645A

   Plasmid

   #74936

   Purpose

   Mammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein)

   Depositor

   Elisa Greggio

   Article

   Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

   Insert

   NSF (NSF Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T645A (mutation in the D2 domain of the protein)

   Promoter

   CMV

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

9. plx304-PHB-V5

   Plasmid

   #122230

   Purpose

   Expresses human PHB attached to a V5 tag

   Depositor

   Alex Kentsis

   Article

   Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

   Insert

   Prohibitin (PHB1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCXN2-HA-AT1R-YFP

    Plasmid

    #101659

    Purpose

    Expresses AT1R with HA tag and YFP in mammalian cells.

    Depositor

    Yusuke Ohba

    Article

    Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

    Insert

    Angiotensin II type 1 receptor (AGTR1 Human)
    

    Tags

    HA and YFP (Venus)

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Bxb1-GA_AIO-GZV-SynZiFTR_mNgn2 donor

    Plasmid

    #229789

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of mouse neurogenin 2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mNgn2; ZF10-NS3-p65 (Neurog2 Mouse)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    ZF10 and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1 blast OFF 3xflag IREB2

    Plasmid

    #169889

    Purpose

    Express 3x flag IREB2, suppressible by doxycycline addition

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    IREB2 (IREB2 Human)
    

    Use

    Lentiviral; Doxycycline mediated suppression

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    Silent mutations in sgIREB2_1 and sgIREB2_2 targe…

    Promoter

    TRE

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Epac-S-H30

    Plasmid

    #170337

    Purpose

    CFP(UST)_EPACdDEPCD_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.

    Insert

    CFP(UST)_EPACdDEPCD_Y(UST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Lyso-PTEN(A4)-mCherry

    Plasmid

    #184054

    Purpose

    Lysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Lyso-PTEN(A4)-mCherry (PTEN Human)
    

    Tags

    Lysosome-associated membrane protein 1 (LAMP1). a…

    Expression

    Mammalian

    Mutation

    Ser 380/385 and Thr 382/383 in PTEN mutated to Al…

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB65 - pL0_tGFP-I (CDS1)

    Plasmid

    #123182

    Purpose

    Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
    

    Use

    Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites; codon-optimised for plants

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBK-CMV-mpx

    Plasmid

    #191826

    Purpose

    mpx probe generation for in situ

    Depositor

    Jason Berman

    Article

    Biggs et al JCI Insight. 2022 Dec 22;7(24):e150849.

    Insert

    mpx cDNA (mpx Zebrafish)
    

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOmnibac_ZZ_linker_ybbR_MAP7_N

    Plasmid

    #159728

    Purpose

    Expresses MAP7 (amino acids 1-316) in Sf9 cells with a ZZ affinity tag and a ybbR tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    MAP7 (only amino acids 1-316)(includes MTBD and P123 domains) (MAP7 Human)
    

    Tags

    ZZ affinity tag and ybbR tag

    Expression

    Insect

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)

    Plasmid

    #153440

    Purpose

    E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc. XDocB binding mode A knock-out mutant
    

    Tags

    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

    Expression

    Bacterial

    Mutation

    Arginine 191 and leucine 195 on XDocB were mutate…

    Promoter

    T7 promoter

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-syn-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234437

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Has Service

    AAV1

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid

    #162877

    Purpose

    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Insert consists of AA1-170

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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