We narrowed to 16,936 results for: She

1. pLXIN2-RFP

   Plasmid

   #99205

   Purpose

   RFP expressing bicistronic retroviral vector

   Depositor

   Saroj Mathupala

   Insert

   Red fluorescent protein
   

   Use

   Retroviral; Bicistronic retroviral expression vec…

   Tags

   Expression

   Mammalian

   Mutation

   The dsRed cDNA was PCR amplified off AddGene plas…

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUA97

   Plasmid

   #69340

   Purpose

   It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy

   Depositor

   Oliver Hobert

   Insert

   ceBFP SEC 3xFlag
   

   Use

   Tags

   3xFlag and ceBFP (mTagBFP)

   Expression

   Worm

   Mutation

   sqt-1(e1350)

   Promoter

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pExp-PpiB

   Plasmid

   #112572

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-PpiB and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHH0103 NEDD4 WW domain #3

   Plasmid

   #104210

   Purpose

   Bacterial expression of WW domain #3 from NEDD4

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   NEDD4 WW domain #3 (NEDD4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHH0103 NEDD4L WW domain #2

   Plasmid

   #104205

   Purpose

   Bacterial expression of WW domain #2 from NEDD4L

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   NEDD4L WW domain #2 (NEDD4L Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGAL-flp(H305L)-NAT

   Plasmid

   #160380

   Purpose

   overexpression of Flp recombinase step-arrest mutant in yeast

   Depositor

   John McCusker

   Insert

   FLP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H305L

   Promoter

   GAL

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLinker-BirA-3xHA-HXGPRT-loxP

   Plasmid

   #86668

   Purpose

   for generation of BirA tagged strains using CRISPR tagging technology

   Depositor

   David Sibley

   Insert

   Linker-BirA-3xHA-HXGPRT 3'UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHH0103 NEDD4 WW domain #1

   Plasmid

   #104208

   Purpose

   Bacterial expression of WW domain #1 from NEDD4

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   NEDD4 WW domain #1 (NEDD4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

   Plasmid

   #191529

   Purpose

   Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   H2BK_mNG_hSLBP(18-108)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mNeonGreen (mNG)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDTA-5

    Plasmid

    #128845

    Purpose

    Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHH0103 NEDD4 WW domain #4

    Plasmid

    #104211

    Purpose

    Bacterial expression of WW domain #4 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #4 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK633

    Plasmid

    #72397

    Purpose

    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole synthetase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKSB- 3xP3-GFPsv40 GGGG AAGA

    Plasmid

    #174531

    Purpose

    3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes

    Depositor

    Eric Marois

    Insert

    3xP3-GFP BsaI cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    3xP3

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB CAG-sfGFP-iCre-ERT2

    Plasmid

    #137856

    Purpose

    CAG-sfGFP-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP-iCre-ERT2
    

    Use

    Cre/Lox; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CbSBS-Flag/TRRAP(S722F)

    Plasmid

    #32105

    Depositor

    Yardena Samuels

    Insert

    Transformation/transcription domain-associated protein (S722F) (TRRAP Human)
    

    Use

    Tags

    Flag 1x

    Expression

    Mammalian

    Mutation

    Changes serine 722 to Phe

    Promoter

    CMV

    Available since

    Nov. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

16. Preprotachykinin A

    Plasmid

    #130664

    Purpose

    Expresses human Preprotachykinin A in mammalian cells

    Depositor

    Michael Kressel

    Insert

    Preprotachykinin A (TAC1 Human)
    

    Use

    Tags

    Fusion Red

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 myc-PITPalpha

    Plasmid

    #58277

    Purpose

    mammalian expression of human PITP alpha

    Depositor

    Shamshad Cockcroft

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pB6-ROSA26-DTA-Capecchi-for

    Plasmid

    #125743

    Purpose

    B6J ROSA26 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21a(+)_C.jejuni m23 peptidase

    Plasmid

    #83297

    Purpose

    Expresses m23 peptidase from Campylobacter jejuni

    Depositor

    SeWon Suh

    Insert

    ERS445056_01343
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-20

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNB3033

    Plasmid

    #113397

    Purpose

    Ty3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducible

    Depositor

    Suzanne Sandmeyer

    Insert

    Ty3 Gag3 codon optimized for E. coli expression
    

    Use

    Tags

    Gag3-6XHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 4IJD

    Plasmid

    #51328

    Depositor

    Cheryl Arrowsmith

    Insert

    4IJD (PRDM9 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pBS-L1PA1CH blast

    Plasmid

    #69612

    Purpose

    Expresses a blasticidin tagged codon optimized L1PA1

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA1
    

    Use

    Tags

    blast cassette

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p2Bac.Myc.p44

    Plasmid

    #39220

    Depositor

    Aziz Sancar

    Insert

    p44 (GTF2H2 )
    

    Use

    Tags

    Myc

    Expression

    Insect

    Mutation

    Promoter

    Pp10

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

24. Flag-TRIM24-F869D

    Plasmid

    #28141

    Depositor

    Michelle Barton

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    A489V, F869D; aa 308 and 309 mutated for shRNA re…

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

25. HT7-mTurquoise2-KDEL

    Plasmid

    #188571

    Purpose

    Expresses fluorescent HaloTag7-mTurquoise2 fusion protein in mammalian cells with localization in the endoplasmic reticulum.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    HaloTag7
    

    Use

    Tags

    KDEL and mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIRES-mRFP-NLS-PCNA (2514)

    Plasmid

    #61850

    Purpose

    cell cycle marker

    Depositor

    Jonathon Pines

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    NLS and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2

    Plasmid

    #171938

    Purpose

    Expresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteins

    Depositor

    David Berson

    Insert

    Apex2, mRuby2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pmr101A-cPr-mCherry (ErmR)

    Plasmid

    #177206

    Purpose

    Plasmid with p15A origin for constitutive mCherry expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Synthetic constitutive promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIC79

    Plasmid

    #63063

    Purpose

    Mammalian expression of EGFP-TEV-S-tag hNSL1 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    hNSL1, IMAGE clone 3997280 (NSL1 Human)
    

    Use

    Tags

    EYFP and TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAGGS-S-B.1.1.529-FLAG

    Plasmid

    #180774

    Purpose

    B.1.1.529 variant S-protein with c-terminal FLAG tag

    Depositor

    Daniel Conway

    Insert

    S-protein B.1.1.529
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pCMV-PE2-pegRNA-tetra-com vector

    Plasmid

    #136271

    Purpose

    Expresses PE2 and Tetra-com modified pegRNA cassette

    Depositor

    Baisong Lu

    Insert

    PE2 and pegRNA cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLL1270

    Plasmid

    #129281

    Purpose

    The vector used for enzyme expression in Thermoanaerobacterium. thermosaccharolyticum throughout this study. Contains the CBP promoter from Clostridium thermocellum

    Depositor

    Lee Lynd

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Clostridium thermocellum DSM1313 Cellobiose Phosp…

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pORTMAGE502B

    Plasmid

    #128971

    Purpose

    Broad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

    Depositor

    George Church

    Inserts

    PapRecT
    mutL E32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    xyl-Pm

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLIX403-EYFP-ccdB-G418

    Plasmid

    #158550

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHH0103 NEDD4L WW domain #3

    Plasmid

    #104206

    Purpose

    Bacterial expression of WW domain #3 from NEDD4L

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4L WW domain #3 (NEDD4L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBS-L1PA13ACHmneo

    Plasmid

    #69613

    Purpose

    Expresses a neomycin tagged codon optimized L1PA13A

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA13A
    

    Use

    Tags

    mneo retrotransposition cassette

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TBRII-C-18

    Plasmid

    #62797

    Purpose

    Localization: Membrane Receptor, C1 Cloning Vector, Excitation:N/A, Emission:N/A

    Depositor

    Michael Davidson

    Insert

    TBRII (TGFBR2 Human)
    

    Use

    Tags

    SNAP Tag

    Expression

    Mammalian

    Mutation

    H385N

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pSil-XIAP

    Plasmid

    #58832

    Purpose

    Express human apoptosis inhibitor XIAP from CAG promoter

    Depositor

    Troy Margrie

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDH-CB-FLPe-P2A-copGFP-T2A-Puro

    Plasmid

    #72258

    Purpose

    Express FLPe, copGFP and Puromycin resistance gene under CB promoter

    Depositor

    Kazuhiro Oka

    Insert

    FLPe-P2A-copGFp-T2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CB and chicken beta-actin + CMV enhancer

    Available since

    Jan. 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. mPA-GFP-Peroxisomes-2

    Plasmid

    #54628

    Purpose

    Localization: Peroxisomes, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    Peroximal Targeting Signal 1 (PTS1) and mPA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

41. N1-LOC8R

    Plasmid

    #66896

    Purpose

    Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.

    Depositor

    Georg Ramm

    Insert

    LOC8R
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pDSAY-vasaCas9sv40

    Plasmid

    #173669

    Purpose

    Cas9 expression vector for Anopheles

    Depositor

    Eric Marois

    Insert

    Cas9 under control of vasa promoter and SV40 terminator
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    vasa

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcB6-Myc-Mfn2 IYFFT

    Plasmid

    #191953

    Purpose

    Expression of Mfn2 protein, in which the C-terminus, after the transmembrane domain, is replaced by a stretch of hydrophobic amino acids (IYFFT)

    Depositor

    Luca Scorrano

    Insert

    Myc-Mfn2-IYFFT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C- terminus replaced by the stretch of hydrophobi…

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pJK408

    Plasmid

    #72422

    Purpose

    Produces Acetobacter aceti 1023 Est10, C-terminal His5 tag

    Depositor

    T. J. Kappock

    Insert

    feruloyl esterase
    

    Use

    Tags

    His5

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pExp-DsbA(SS)

    Plasmid

    #112563

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-DsbA(SS) and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBS SK(+)/mGATA2

    Plasmid

    #83823

    Purpose

    mGATA2 cDNA

    Depositor

    Douglas Engel

    Insert

    GATA2 (Gata2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

47. pAAV_hsyn_FLEX_tau-APEX2

    Plasmid

    #171937

    Purpose

    Expresses APEX2 linked to tau for labeling microtubules

    Depositor

    David Berson

    Insert

    tau-APEX2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hsyn

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. HA-tagged Abr

    Plasmid

    #38190

    Depositor

    Nora Heisterkamp

    Insert

    ABR (ABR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

49. pET StrepII TEV cloning vector with BioBrick polycistronic restriction sites (9R)

    Plasmid

    #48290

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. RV-Syn-Dio-mVenus

    Plasmid

    #153204

    Purpose

    Can be used to generate retrovirus that will mark the membrane (Palmitoylated-Venus) in the presence of Cre from the rat synapsin promoter

    Depositor

    Lynette Lim

    Insert

    membrane-tagged-Venus
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    rat synapsin

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge