Addgene

Showing: 1821 - 1840 of 48478 results

Results per page:

Sort results by:

pHCas9-Nours

Plasmid

#107733

Purpose

Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

Depositor

Sheng Yang

Article

Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Human optimized

Promoter

TEF1

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-pEF1a-DIO-tTAc

Plasmid

#172126

Purpose

Expression of InteinC-tTAC in Cre recombinase positive cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

InteinC-tTAc

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKII

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-FRT-EBFP-tTAn

Plasmid

#172127

Purpose

Expression of EBFP and tTA-InteinN in Flp recombinase positive cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EBFP, tTAn-InteinN

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-PGK-LexA-CIB1-biLINuS-P2A-tagBFP

Plasmid

#192609

Purpose

Lentiviral vector for constitutive expression of LexA-CIB1-biLINuS-P2A-tagBFP.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

LexA-CIB1-biLINuS-P2A-tagBFP

Use

Lentiviral and Synthetic Biology

Tags

tagBFP

Expression

Mutation

Promoter

PGK

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TC1464

Plasmid

#164881

Purpose

CMV-bpNLS-dCas13d-bpNLS-ADARdd, ADARdd embeded in loop1

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

dcas13d-ADARdd

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Sha2Cas9-HF

Plasmid

#192142

Purpose

Expresses highly specific Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9-HF

Use

AAV

Tags

Expression

Mammalian

Mutation

Sha2Cas9-HF (R247A and N415A)

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Sha2Cas9

Plasmid

#192140

Purpose

Expresses Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SpeCas9

Plasmid

#192139

Purpose

Expresses SpeCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

SpeCas9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-NLS-RfxCas13d-mcherry-NLS-Flag

Plasmid

#165077

Purpose

overexpression of RfxCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

RfxCas13d

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-NES-LwaCas13a-msfGFP-NES-Flag

Plasmid

#165070

Purpose

overexpression of LwaCas13a in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

LwaCas13a

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-NES-RfxCas13d-msfGFP-NES-Flag

Plasmid

#165076

Purpose

overexpression of RfxCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

RfxCas13d

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX601-AAV-CMV-NLS-ISAam1-NLS-3xHA-bGHpA;U6-BbsI-reRNA

Plasmid

#205316

Purpose

All-in-one AAV vector expressing ISAam1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

humanized ISAam1 TnpB

Use

AAV

Tags

3 x HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-NES-EsCas13d-msfGFP-NES-Flag

Plasmid

#165074

Purpose

overexpression of EsCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

EsCas13d

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX601-AAV-CMV-NLS-ISDge10-NLS-3xHA-bGHpA;U6-BsaI-reRNA

Plasmid

#205318

Purpose

All-in-one AAV vector expressing ISDge10 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

humanized ISDge10 TnpB

Use

AAV

Tags

3 x HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

Plasmid

#205317

Purpose

All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

humanized ISYmu1 TnpB

Use

AAV

Tags

3 x HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET42b-ABE7.10

Plasmid

#120398

Purpose

Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

Junjiu Huang

Article

Liang et al Nat Commun. 2019 Jan 8;10(1):67. doi: 10

Insert

ABE7.10

Use

Tags

His

Expression

Bacterial

Mutation

Mammalian codon-optimized

Promoter

T7

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(-)-ABE7.10

Plasmid

#120399

Purpose

Expresses ABE7.10 in mammalian cells

Depositor

Junjiu Huang

Article

Liang et al Nat Commun. 2019 Jan 8;10(1):67. doi: 10

Insert

ABE7.10

Use

Tags

Expression

Mammalian

Mutation

Mammalian codon-optimized

Promoter

CMV

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
enIscB

Plasmid

#205410

Purpose

Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

Insert

pCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry

Use

Tags

Expression

Mammalian

Mutation

E85R+H369R+S387R+S457R

Promoter

CAG, hU6, CMV

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX601-AAV-CMV-NLS-ISAba30-NLS-3xHA-bGHpA;U6-BbsI-reRNA

Plasmid

#205320

Purpose

All-in-one AAV vector expressing ISAba30 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

humanized ISAba30 TnpB

Use

AAV

Tags

3 x HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX601-AAV-CMV-NLS-ISTfu1-NLS-3xHA-bGHpA;U6-BbsI-reRNA

Plasmid

#205319

Purpose

All-in-one AAV vector expressing ISTfu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

humanized ISTfu1 TnpB

Use

AAV

Tags

3 x HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge