We narrowed to 4,495 results for: Ado

1. pLenti-VRER-PGK-Puro

   Plasmid

   #110856

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRER
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP Kind2 S159/666E

   Plasmid

   #105313

   Purpose

   To study altered downstream functions of phospholylation-site mimetic kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159/666E

   Promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP KInd2 S159E

   Plasmid

   #105311

   Purpose

   To study altered downstream functions of phospholylation-site mimetic kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159E

   Promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP Kind2 S159/666A

   Plasmid

   #105309

   Purpose

   To study altered downstream functions of phospholylation-site null kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159/666A

   Promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP Kind2 S666A

   Plasmid

   #105308

   Purpose

   To study altered downstream functions of phospholylation-site null kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S666A

   Promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP KInd2 S159A

   Plasmid

   #105306

   Purpose

   To study altered downstream functions of phospholylation-site null kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159A

   Promoter

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-Basic-Fascin-Promoter (-210-0)

   Plasmid

   #89826

   Purpose

   Fascin promoter for luciferase assay

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

   Insert

   Fascin1 (FSCN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available Since

   May 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CRISPRoff-v2.1

   Plasmid

   #167981

   Purpose

   Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
   

   Use

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. TETv4

   Plasmid

   #167983

   Purpose

   Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   TETv4 (TET1CD-XTEN80-dCas9)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   3xNLS, TET1 catalytic domain, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCJ136

    Plasmid

    #162665

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pInducer20 ALPK1 Y254C

    Plasmid

    #231587

    Purpose

    ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    changed tyrosine 254 to cysteine

    Promoter

    doxycycline

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pInducer20 ALPK1 V1092A

    Plasmid

    #231588

    Purpose

    ALPK1 gene mutated in position V1092A (mutation found in spiradenomacarcinoma (sweat gland cancer)) under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    changed Valine1092 to Alanine

    Promoter

    doxycycline

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInducer20 ALPK1 T237M

    Plasmid

    #231586

    Purpose

    ALPK1 gene mutated in position T237M (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    changed threonine 237 to Methionine

    Promoter

    doxycycline

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO-Tet-On-shRNA-Control

    Plasmid

    #98398

    Purpose

    Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    non-targeting randomized sequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Villinpromoter-blue-FlpOERT2

    Plasmid

    #67278

    Purpose

    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

    Insert

    Villin-mTQ2-P2A-FlpO-ERT2
    

    Use

    Tags

    mTQ2, linked to FlpO through an P2A ribosomal ski…

    Expression

    Mammalian

    Mutation

    Promoter

    Villin

    Available Since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-6p1-GST-ArcFL

    Plasmid

    #119877

    Purpose

    Bacterial Expression construct for protein production and purification

    Depositor

    Jason Shepherd

    Article

    Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

    Insert

    Activity Regulated Cytoskeletal Protein (Arc Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-Tox2-IRES-eGFP

    Plasmid

    #165428

    Purpose

    Expresses TOX2 in mammalian cells

    Depositor

    Anjana Rao, Patrick Hogan

    Article

    Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

    Insert

    TOX2 (Tox2 Mouse)
    

    Use

    Tags

    3xflag 5' of Tox2

    Expression

    Mammalian

    Mutation

    Promoter

    5' LTR

    Available Since

    March 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX_hDDX5_FL_wt

    Plasmid

    #88871

    Purpose

    inducible expression of GST-DDX5 in bacteria

    Depositor

    Dan Littman

    Article

    Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 AIF 1-90 mCherry FRB

    Plasmid

    #67530

    Purpose

    For expression of mCherry fused FRB to the mitochondrial innermembrane

    Depositor

    Stephen Tait

    Article

    Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

    Inserts

    AIF a.a. 1 - 90
    mCherry
    FRB
    

    Use

    Tags

    AIF 1 - 90, FRB, and mCherry

    Expression

    Mammalian

    Mutation

    T->L

    Promoter

    CMV

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBA904

    Plasmid

    #122238

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs1 in stem loop 2 of the constant region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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