-
Plasmid#194295PurposeIn vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LACDepositorInsertcavin1b (cavin1b Zebrafish)
UseIn vitro transcription of mrnaTagsExpressionMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEXP1-DEST-Noa1-HIS
Plasmid#191972PurposeDestination vector containing HIS-tagged NOA1 coding sequence.DepositorInsertNoa1-HIS (NOA1 Human)
UseSynthetic BiologyTagsHisExpressionMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
mKate2-DrCavin1a in pT3TS-Dest
Plasmid#194294PurposeIn vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAIDepositorInsertcavin1a (cavin1a Zebrafish)
UseIn vitro transcription of mrnaTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DEST47-Noa1-GFP
Plasmid#191968PurposeGateway Destination Vector, containing NOA1-GFP chimera coding sequence, for Gateway Cloning TechnologyDepositorInsertNoa1-GFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Dest-MBP-Nsp1
Plasmid#191483PurposeMammalian expression of MBP-tagged SARS-CoV-2 Nsp1DepositorInsertMBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::HA-RfA
Plasmid#186404PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pDEST-pSP172BSSPE-pFOGc::RfA
Plasmid#186397PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA
Plasmid#186396PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-RfA-Venus
Plasmid#186372PurposeDestination vector for the expression of a fusion of an N-ter protein of interest with Venus into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-Venus-RfA
Plasmid#186371PurposeDestination vector for the expression of a fluorescent fusion of N-ter Venus and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST tol2 UbB polyA
Plasmid#188701PurposeGateway destination vector containing the zebrafish ubiquitin B polyadenylation sequenceDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-HA
Plasmid#186405PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDEST-pSP172BSSPE-pFOGc::RfA-HA
Plasmid#186406PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdestMB14_hsp-16.2p-grh-1-gfp
Plasmid#177762PurposeOverexpression of C. elegans grh-1DepositorInsertgrh-1 (grh-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos2-dVHH[GFP] in pCSDEST
Plasmid#162870PurposeSuper-resolution of GFP in cultureDepositorInsertmEos2-dVHH[EGFP]
UseTagsmEos2ExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-313 (attR4-attR3)
Plasmid#161898PurposeMultisite Gateway (attR4-attR3) vector for mammalian protein expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3566 (pDEST15_Spt6 1223-1451)
Plasmid#91814PurposeBacterial expression of residues 1223-1451 from S. cerevisiae Spt6DepositorInsertSPT6 (SPT6 Budding Yeast)
UseTagsGSTExpressionBacterialMutationdeleted residues 1-1222PromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-894 (attR4-attR2)
Plasmid#161893PurposeMultisite Gateway (attR4-attR2) vector for expression of multiple proteins in E. coliDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-639 (attR4-attR2)
Plasmid#161880PurposeMultisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only