We narrowed to 4,947 results for: mcs

1. pENeGFP PCNAL2 pc653

   Plasmid

   #167564

   Purpose

   The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

   Depositor

   Cristina Cardoso

   Article

   Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

   Insert

   Proliferating Cell Nuclear Antigen (PCNA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV5-rat ERK2-K52R

   Plasmid

   #40813

   Depositor

   Natalie Ahn

   Article

   Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

   Insert

   MAPK1 (Mapk1 Rat)
   

   Tags

   His6

   Expression

   Mammalian

   Mutation

   K52R

   Promoter

   CMV

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

3. EGFP-uTEV1-MAVS

   Plasmid

   #170998

   Purpose

   HiLITR protease with MAVS c-terminal tmd targeting information (Mitochondria)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-MAVS(tmd)
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3537 pMIG Bim

   Plasmid

   #8786

   Depositor

   Stanley Korsmeyer

   Article

   Cheng et al Mol Cell 2001 Sep;8(3):705-11.

   Insert

   Bim (Bcl2l11 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pAF0286 Ef1a-Cp36_attB-BFP acceptor

   Plasmid

   #193474

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Cp36_attB
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-hPMCA2z/b

   Plasmid

   #47584

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   pMCA2z/b (ATP2B2 Human)
   

   Tags

   EGFP-HA-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

7. AV4-EF1-NMNAT2

   Plasmid

   #124970

   Purpose

   Express NMNAT2

   Depositor

   Kazuhiro Oka

   Article

   Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

   Insert

   NMNAT2, GFP (NMNAT2 Human)
   

   Use

   AAV

   Promoter

   EF1-alpha

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS4.1w-tet-on-NORAD

   Plasmid

   #125010

   Purpose

   express in mammalian cell

   Depositor

   Ruey-Hwa Chen

   Article

   Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

   Insert

   Homo sapiens non-coding RNA activated by DNA damage (NORAD), long non-coding RNA (NORAD Human)
   

   Expression

   Mammalian

   Promoter

   TRE-Tight

   Available Since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Bov.hsc70.RSET.FL.wt(NarI)

   Plasmid

   #12532

   Depositor

   David McKay

   Article

   Ha et al J Biol Chem. 1997 Oct 31. 272(44):27796-

   Insert

   bovine 70 kDa heat shock cognate protein full length wild type
   

   Expression

   Bacterial

   Mutation

   wild type

   Available Since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNP1 tdTomato toehold switch

    Plasmid

    #107354

    Purpose

    Expresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 5 + tdTomato
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-EF1a-tetR-T2A-iRFP

    Plasmid

    #176028

    Purpose

    construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    tetR
    iRFP
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. G1397 DddAtox-C–dSpCas9

    Plasmid

    #157836

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
    

    Use

    CRISPR

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV5-ratERK2-Q103A

    Plasmid

    #40816

    Depositor

    Natalie Ahn

    Article

    Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

    Insert

    MAPK1 (Mapk1 Rat)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    Q103A

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pCMV Gab1(PRD)-mCherry-HOTag3

    Plasmid

    #190026

    Purpose

    Transiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.

    Depositor

    Lukasz Bugaj

    Article

    Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

    Insert

    Gab1(PRD) CluMPS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Pu2-3::tag::sl1(G16C)

    Plasmid

    #79002

    Purpose

    Expression of exogenous SL1 in multiple tissues of C. elegans

    Depositor

    Xiao Liu, Ge Zhan

    Article

    Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

    Insert

    sls-1.2 (sls-1.2 Nematode)
    

    Tags

    illumina-adapter

    Expression

    Worm

    Mutation

    G16C

    Promoter

    F08G2.17

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL1-PD-4xDQNAT

    Plasmid

    #128391

    Purpose

    In vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Protein D
    

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNP1 Aqua toehold switch

    Plasmid

    #107356

    Purpose

    Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 8 + Aquamarine
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPR-v2-FE-PuroR

    Plasmid

    #186746

    Purpose

    Modified lentiCRISPRv2 3rd generation lentiviral backbone (Addgene Cat# 52961) containing an FE hairpin

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX459-HypaCas9-TUBA1B_sgRNA

    Plasmid

    #183889

    Purpose

    pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459-HypaCas9-mR2-TUBA1B_sgRNA

    Plasmid

    #183888

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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