We narrowed to 7,531 results for: ski

1. pEN_TT 3xFLAG-MAVS-Gln93,Ala271

   Plasmid

   #158632

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Mutation

   E93Q/Q271A

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN_TT 3xFLAG-MAVS-Ala148,Ala271

   Plasmid

   #158633

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Mutation

   Q148A/Q271A

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro

   Plasmid

   #158639

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Mutation

   Q148A/Q271A

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLIK 3xFLAG-MAVS-Ala148 hygro

   Plasmid

   #158636

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Mutation

   Q148A

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLIK 3xFLAG-MAVS-Gln93 hygro

   Plasmid

   #158635

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Mutation

   E93Q

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA

   Plasmid

   #113696

   Purpose

   U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA

   Plasmid

   #113695

   Purpose

   U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pscALPSpuro-MmACE2 (Mouse)

   Plasmid

   #158087

   Purpose

   Expresses mouse ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Mouse ACE2 (Ace2 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

   Plasmid

   #113691

   Purpose

   U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   NLS

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGS_101

    Plasmid

    #160541

    Purpose

    Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

    Depositor

    Susan Lindquist, Li-Huei Tsai

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e4 (APOE Human)
    

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e4 allele

    Promoter

    GAL1

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro

    Plasmid

    #89992

    Purpose

    Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

    Insert

    OCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)
    

    Use

    CRISPR

    Promoter

    none

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pscALPSpuro-MjACE2 (Pangolin)

    Plasmid

    #158084

    Purpose

    Expresses pangolin ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Pangolin ACE2 (ACE2 )
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGS_128

    Plasmid

    #160651

    Purpose

    Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

    Depositor

    Susan Lindquist, Li-Huei Tsai

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e3 (APOE Human)
    

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e3 allele

    Promoter

    pZ estradiol inducible promoter

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

    Plasmid

    #51248

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    amino acids 849,865,877,907,980,1006 mutated to A…

    Promoter

    EF1a

    Available Since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pscALPSpuro-HsACE2 (human)

    Plasmid

    #158081

    Purpose

    Expresses human ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Human ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-mCherry-DMDEx23-eGFP

    Plasmid

    #211366

    Purpose

    Piggybac transposon plasmid for a DMD exon 23 skipping reporter (mCherry-DMDEx23)

    Depositor

    Ulrich Lächelt

    Article

    Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

    Insert

    mCherry-DMDEx23-eGFP
    

    Expression

    Mammalian

    Mutation

    mCherry interrupted by mdx dystrophin exon 23 bet…

    Promoter

    CAG

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGS_107

    Plasmid

    #160650

    Purpose

    Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

    Depositor

    Susan Lindquist, Li-Huei Tsai

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e4 (APOE Human)
    

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e4 allele

    Promoter

    GAL1

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-EGFP MASTL G44S (siRNA resistant)

    Plasmid

    #191012

    Purpose

    Expresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

    Depositor

    Johanna Ivaska

    Article

    Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Microtubule-associated serine/threonine kinase like (MASTL Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    G44S

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGS_129

    Plasmid

    #160652

    Purpose

    Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

    Depositor

    Susan Lindquist, Li-Huei Tsai

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e4 (APOE Human)
    

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e4 allele

    Promoter

    pZ estradiol inducible promoter

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDH-EMTB-TurboID-V5-puro

    Plasmid

    #190741

    Purpose

    A lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    EMTB-TurboID-V5 (RPS14 EMTB is human ensconsin; TurboID is engineered BirA from E.coli, Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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