We narrowed to 2,115 results for: PORA

1. pmScarlet3_C1

   Plasmid

   #189753

   Purpose

   C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAH-POLY

   Plasmid

   #64915

   Purpose

   Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

   Depositor

   Huiwang Ai

   Article

   Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

   Inserts

   Polyspecific aminoacyl-tRNA Synthetase
   Amber Suppression tRNA
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6/H1

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTrichoGate-2

   Plasmid

   #160702

   Purpose

   gpdh promoter from Trichoderma virens bordered by BsaI restriction sites

   Depositor

   Artemio Mendoza

   Article

   Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

   Insert

   gpdh
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Incorporation of BsaI restriction sites

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBCCP-GFP-NLS

   Plasmid

   #173923

   Purpose

   Fluorescent labeling of the nuclear envelope

   Depositor

   Shinji Sueda

   Article

   Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

   Insert

   Biotin carboxyl carrier protein, AcGFP1
   

   Use

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSerOTS-C1* (V70)

   Plasmid

   #188537

   Purpose

   Phosphoserine incorporation into recombinant proteins

   Depositor

   Jesse Rinehart

   Article

   Mohler et al Mol Syst Biol. 2023 Aug 8;19(8):e10591.

   Inserts

   pSerRS
   Ef-pSer
   tRNApSer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutations resulting from laboratory evolution

   Promoter

   glnS*

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRE4-Bpa

   Plasmid

   #155342

   Purpose

   Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

   Depositor

   Irene Coin

   Article

   Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

   Insert

   humanized EBpaRS/BstYam
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK/U6

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSF-G1(6/7FTrp)RS

   Plasmid

   #207621

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSF-G1(4/5FTrp)RS

   Plasmid

   #207620

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. MmXYRS-4xM15

   Plasmid

   #155343

   Purpose

   Plasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.

   Depositor

   Irene Coin

   Article

   Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

   Insert

   Humanized MmXYRS/tRNAM15
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/U6

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lys-eMagB-iRFP

    Plasmid

    #162249

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    p18-eMagB-iRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. optoAKT

    Plasmid

    #207164

    Purpose

    Light-inducible expression of optoAKT; for precise temporal control of the AKT signaling pathway

    Depositor

    Kai Zhang

    Article

    Wang et al Elife. 2020 Oct 6;9:e57395. doi: 10.7554

    Insert

    CRY2-mCherry-AKT(_PH)-2A-CIBN2-GFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ER-EGFP-eMagA

    Plasmid

    #162245

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    CytP450 ER targeting sequencel-EGFP-eMagA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. eMagB-iRFP-Mito

    Plasmid

    #162250

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagB-iRFP-OMP25
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ER-mCherry-eMagA

    Plasmid

    #162248

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    CytP450 ER targeting sequencel-mCherry-eMagA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. eMagA-mCherry-Mito

    Plasmid

    #162251

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagA-mCherry-OMP25
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKERep

    Plasmid

    #211826

    Purpose

    Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKESa

    Plasmid

    #211828

    Purpose

    Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TagRFP-T-VAPB(1- 218)- eMagB

    Plasmid

    #162255

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    TagRFP-T-VAPB(1- 218)- eMagB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL4.27_GRHL-SPE

    Plasmid

    #177774

    Purpose

    Luciferase reporter for GRHL

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMAH-EcaYRS

    Plasmid

    #153558

    Purpose

    Amber suppression for incorporating 3-aminotyrosine to proteins in mammalian cells

    Depositor

    Huiwang Ai

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

    Inserts

    EcaYRS
    amber suppression tRNA (multiple copies)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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